公开(公告)号：EP4269576A1

公开(公告)日：2023-11-01

申请号：EP21918767.1

申请日：2021-03-02

申请人： Asymchem Life Science (Tianjin) Co., Ltd.

发明人： HONG, Hao ,  JAMES, Gage ,  XIAO, Yi ,  ZHANG, Na ,  JIAO, Xuecheng ,  JIA, Ru ,  LIU, Wenjing ,  LIU, Ye

IPC分类号： C12N9/20 ,  C12N15/55 ,  C12P7/40

摘要： Provided are a lipase mutant and use thereof. The amino acid sequence of the lipase mutant has a sequence shown in SEQ ID NO: 1, that is, sites at which amino acid mutations occur include a V154L site. The protein structure and function of the lipase mutant are changed, the solubility expressed in Escherichia coli is improved, and the catalytic activity of the enzyme is improved. In practical applications, the usage amount of the enzyme is reduced, the reaction volume is reduced, the difficulty of post-treatment is reduced, and it is suitable for industrial production.

公开(公告)号：EP4394035A1

公开(公告)日：2024-07-03

申请号：EP21956530.6

申请日：2021-10-13

申请人： Asymchem Life Science (Tianjin) Co., Ltd

发明人： HONG, Hao ,  JAMES, Gage ,  ZHANG, Na ,  JIAO, Xuecheng ,  LIU, Fang ,  JIA, Ru ,  LIU, Wenjing

IPC分类号： C12N9/20 ,  C12N15/55 ,  C12N15/70 ,  C12N1/21 ,  C12P7/02 ,  C12P7/40 ,  C12R1/19

摘要： Provided are a lipase mutant and an application thereof. Specifically, one or more mutations selected from A262H, A338V, V364I, A158P/V, and I159N are generated on the basis of an amino acid sequence as shown in SEQ ID NO: 1; and compared with a parental lipase, a change in the structure and function of a protein occurs in the lipase mutant, and the stereoselectivity is improved, such that a usage amount of an enzyme is decreased to a certain extent, the post-treatment difficulty is reduced, and the lipase mutant is suitable for industrial production.