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公开(公告)号：EP4438723A1
公开(公告)日：2024-10-02
申请号：EP22919603.5
申请日：2022-02-14
发明人： HONG, Hao , JAMES, Gage , XIAO, Yi , ZHANG, Na , PAN, Long , MA, Liteng , CUI, Yuxia , GAO, Yanyan
IPC分类号： C12N9/02 , C12N9/06 , C12N11/091
摘要： Provided are an immobilized enzyme and an application thereof in continuous production. The immobilized enzyme is a Polyethyleneimine (PEI)-modified immobilized enzyme, and includes: an enzyme, which includes Amine Dehydrogenase (AmDH) and/or Formate Dehydrogenase (FDH); and a carrier, which is a cyanuric chloride-activated amino carrier. The problem in the prior art of poor performance of an immobilized enzyme is solved, the catalytic activity and reusability of the immobilized enzyme are improved, and the immobilized enzyme is suitable for the field of enzyme immobilization.
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公开(公告)号：EP4276178A1
公开(公告)日：2023-11-15
申请号：EP21922007.6
申请日：2021-03-02
发明人： HONG, Hao , JAMES, Gage , XIAO, Yi , ZHANG, Na , JIAO, Xuecheng , YANG, Yiming , WANG, Xiang , ZHAO, Junqi
摘要： Provided are an esterase mutant and use thereof. The amino acid sequence of the esterase mutant has a sequence as shown in SEQ ID NO: 1, and sites at which amino acid mutations occur include an N51G site. The esterase mutant achieves changes of the structure and function of a protein, improves both enzyme specificity and enzyme activity, reduces the usage amount of the enzyme, and reduces the industrial production cost.
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公开(公告)号：EP4368732A2
公开(公告)日：2024-05-15
申请号：EP24165738.6
申请日：2020-07-08
发明人： HONG, Hao , JAMES, Gage , XIAO, Yi , ZHANG, Na , LIU, Fang , WANG, Zujian , SHI, Wanming , YAN, Junjie , ZHANG, Mujiao
IPC分类号： C12R1/19
CPC分类号： C12N9/0004 , C12N15/70 , C12P7/02 , C12Y101/01 , C12R2001/1920210501
摘要： Provided is a ketoreductase mutant and applications thereof. Compared with an amino acid sequence shown in SEQ ID NO: 1, the amino acid sequence of the ketoreductase mutant includes at least one of the following mutation sites: L198P, S96W/I/L/V, M194L or L1526, herein "/" means "or." The ketoreductase mutant provides high selectivity and high activity with respect to a substrate ketone, and the application of the mutant in a catalytic reduction reaction of a ketone substrate may increase the production efficiency of a chiral alcohol compound corresponding thereto.
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4.发明公开
公开(公告)号：EP4083201A1
公开(公告)日：2022-11-02
申请号：EP20914725.5
申请日：2020-01-14
发明人： HONG, Hao , JAMES, Gage , XIAO, Yi , RAJASEKAR, Vyasarayani Williams , CUI, Yuxia , ZHANG, Na , ZHAO, Jiadong , GAO, Yanyan
IPC分类号： C12N11/08 , C12N11/089
摘要： Disclosed are a modified epoxy resin immobilized enzyme, a preparation method therefor and an application thereof. Herein, the preparation method includes the following steps: modifying an epoxy resin, adding a polyethyleneimine to a modified epoxy resin for further modification, and then adding an enzyme to be immobilized and a glutaraldehyde for immobilization, to obtain the modified epoxy resin immobilized enzyme. The epoxy resin is modified, the polyethyleneimine is added to the modified epoxy resin for the further modification, and an aldehyde group in the resin and an amino group in the polyethyleneimine are covalently bound to each enzyme, then it is activated by the bifunctional reagent glutaraldehyde. In this way, a steric resin arm is increased, to form a network structure, it may be more easily bound to the enzyme by covalent binding, and the enzyme load may also be improved because the steric inhibition is reduced.
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公开(公告)号：EP4261280A1
公开(公告)日：2023-10-18
申请号：EP21920431.0
申请日：2021-03-02
发明人： HONG, Hao , JAMES, Gage , XIAO, Yi , ZHANG, Na , JIAO, Xuecheng , LI, Rui , ZHANG, Yanqing , YANG, Yiming
摘要： Provided are an amino acid dehydrogenase mutant and use thereof. The amino acid sequence of the amino acid dehydrogenase mutant has a sequence as shown in SEQ ID NO: 1, and sites at which amino acid mutations occur include a K144G site. According to the amino acid dehydrogenase mutant, changes in protein structure and function are achieved, the enzyme activity is improved, and the usage amount of amino acid dehydrogenase is reduced. In addition, the enzyme specificity is also correspondingly improved, and the cost in industrial production of amino acids is reduced.
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公开(公告)号：EP4183878A1
公开(公告)日：2023-05-24
申请号：EP21842025.5
申请日：2021-02-18
发明人： HONG, Hao , JAMES, , Gage , XIAO, Yi , ZHANG, Na , VYASA, Williams , CUI, Yuxia , ZHAO, Jiadong , GAO, Yanyan
IPC分类号： C12N11/084
摘要： Provided are a PVA membrane immobilized enzyme and a preparation method therefor. The PVA membrane immobilized enzyme comprises a PVA porous membrane and an enzyme entrapped on the PVA porous membrane. The PVA porous membrane is a three-dimensional structured PVA porous membrane. The enzyme is any one selected from transaminase, D-lactate dehydrogenase, cyclohexanone monooxygenase, ketoreductase, alkene reductase, nitrilase, ammonia lyase, amino acid dehydrogenase, imine reductase, alcohol dehydrogenase, ammonium formate dehydrogenase, glucose 1-dehydrogenase and mutants thereof. The three-dimensional structured PVA porous membrane is used as a carrier to immobilize an enzyme in an entrapment manner. The entrapment and immobilization process is simple, conditions are mild, and the specific surface area is large. After entrapping and immobilizing the enzyme in the PVA porous membrane, the enzyme is stable, and cannot be easily leached out in the process of use. The porous structure of the PVA porous membrane used can better transmit reactants and products, is suitable for use in continuous flow biochemical catalysis, and has wide applicability to enzymes.
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公开(公告)号：EP4144842A1
公开(公告)日：2023-03-08
申请号：EP20933801.1
申请日：2020-05-29
发明人： HONG, Hao , JAMES, Gage , XIAO, Yi , ZHANG, Na , RAJASEKAR, Vyasarayani Williams , CUI, Yuxia , ZHAO, Jiadong , GAO, Yanyan , FU, Han
IPC分类号： C12N11/089 , C12N11/098 , C12N11/18
摘要： Provided are an immobilized enzyme, and a preparation method and a use thereof. The immobilized enzyme includes activated PEI and an enzyme covalently bonded to the activated PEI, herein the enzyme is selected from any one of a transaminase, a ketoreductase, a monooxygenase, an ammonia lyase, an ene-reductase, an imine reductase, an amino acid dehydrogenase and a nitrilase.
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公开(公告)号：EP4368733A3
公开(公告)日：2024-10-23
申请号：EP24165741.0
申请日：2020-07-08
发明人： HONG, Hao , JAMES, Gage , XIAO, Yi , ZHANG, Na , LIU, Fang , WANG, Zujian , SHI, Wanming , YAN, Junjie , ZHANG, Mujiao
摘要： Provided is a ketoreductase mutant and applications thereof. Compared with an amino acid sequence shown in SEQ ID NO: 1, the amino acid sequence of the ketoreductase mutant includes at least one of the following mutation sites: L198P, S96W/I/L/V, M194L or L1526, herein "/" means "or." The ketoreductase mutant provides high selectivity and high activity with respect to a substrate ketone, and the application of the mutant in a catalytic reduction reaction of a ketone substrate may increase the production efficiency of a chiral alcohol compound corresponding thereto.
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公开(公告)号：EP4434965A1
公开(公告)日：2024-09-25
申请号：EP22932813.3
申请日：2022-04-13
发明人： HONG, Hao , JAMES, Gage , XIAO, Yi , JIAO, Jianye , YAN, Honglei , ZHANG, Tao , LIU, Bin , LIN, Han , XIONG, Zhengchang
IPC分类号： C07D207/20
CPC分类号： Y02P20/55 , C07D207/20
摘要： The present disclosure discloses a method for continuously synthesizing N-Boc-2,5-dihydropyrrole. The method includes the following steps: cis-1,4 dichloro-2 butene is dissolved in an organic solvent, to prepare solution A; urotropine is dissolved in an organic solvent, to prepare solution B; the solution A and solution B are fed into a first continuous reaction device; a product overflow system of the first continuous reaction device is transferred to a second continuous reaction device, and concentrated hydrochloric acid is fed into the same; a product overflow system of the second continuous reaction device is transferred to a third continuous reaction device, and potassium carbonate is fed into the same; a product overflow system of the third continuous reaction device is transferred to a fourth continuous reaction device, and Boc acid anhydride solution is fed into the same; and a product of the fourth continuous reaction device is subjected to continuous solid-liquid separation, concentration and distillation, to obtain N-Boc-2,5-dihydropyrrole. By using technical solution of the present disclosure, the route involving high-risk materials is avoided, and the production capacity and yield are improved in a continuous manner.
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公开(公告)号：EP4368732A3
公开(公告)日：2024-08-28
申请号：EP24165738.6
申请日：2020-07-08
发明人： HONG, Hao , JAMES, Gage , XIAO, Yi , ZHANG, Na , LIU, Fang , WANG, Zujian , SHI, Wanming , YAN, Junjie , ZHANG, Mujiao
CPC分类号： C12N9/0004 , C12N15/70 , C12P7/02 , C12Y101/01 , C12R2001/1920210501
摘要： Provided is a ketoreductase mutant and applications thereof. Compared with an amino acid sequence shown in SEQ ID NO: 1, the amino acid sequence of the ketoreductase mutant includes at least one of the following mutation sites: L198P, S96W/I/L/V, M194L or L1526, herein "/" means "or." The ketoreductase mutant provides high selectivity and high activity with respect to a substrate ketone, and the application of the mutant in a catalytic reduction reaction of a ketone substrate may increase the production efficiency of a chiral alcohol compound corresponding thereto.
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