公开(公告)号：EP1947184B1

公开(公告)日：2011-03-30

申请号：EP08005377.0

申请日：2004-02-20

申请人： Athenix Corporation

发明人： Carozzi, Nadine ,  Hargiss, Tracy ,  Koziel, Michael G. ,  Duck, Nicholas B. ,  Carr, Brian

IPC分类号： C12N15/32 ,  C12N15/82 ,  C12N5/14 ,  C12N15/63 ,  A01H1/00 ,  C07K14/325 ,  C07K16/12 ,  A01N63/00

CPC分类号： C12N15/8286 ,  C07K14/325 ,  Y02A40/162

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin and delta-endotoxin-associated polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin and delta-endotoxin-associated nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequences shown in SEQ ID NOS: 3, 5, 7, 9, 11, 14, 16, 18, 20, 22, 24, 27, and 29, and the nucleotide sequences set forth in SEQ ID NOS: 1, 2, 4, 6, 8, 10, 12, 13, 15, 17, 19, 21, 23, 25, 26, and 28, as well as variants and fragments thereof.

公开(公告)号：EP1947184A3

公开(公告)日：2008-10-22

申请号：EP08005377.0

申请日：2004-02-20

申请人： Athenix Corporation

发明人： Carozzi, Nadine ,  Hargiss, Tracy ,  Koziel, Michael G. ,  Duck, Nicholas, B. ,  Carr, Brian

IPC分类号： C12N15/32 ,  C12N15/82 ,  C12N5/14 ,  C12N15/63 ,  A01H1/00 ,  C07K14/325 ,  C07K16/12 ,  A01N63/00

CPC分类号： C12N15/8286 ,  C07K14/325 ,  Y02A40/162

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin and delta-endotoxin-associated polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin and delta-endotoxin-associated nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequences shown in SEQ ID NOS: 3, 5, 7, 9, 11, 14, 16, 18, 20, 22, 24, 27, and 29, and the nucleotide sequences set forth in SEQ ID NOS: 1, 2, 4, 6, 8, 10, 12, 13, 15, 17, 19, 21, 23, 25, 26, and 28, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子杀虫活性的组合物和方法。 提供了包含δ-内毒素和δ-内毒素相关多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中用于在植物和细菌中转化和表达。 组合物还包含转化的细菌，植物，植物细胞，组织和种子。 具体而言，提供了分离的δ-内毒素和δ-内毒素相关的核酸分子。 另外，包含对应于多核苷酸的氨基酸序列。 具体而言，本发明提供了分离的核酸分子，其包含编码SEQ ID NOS： 和29所示的核苷酸序列，以及SEQ ID NO：1,2,4,6,8,10,12,13,15,17,19,21,23,25,26和28所示的核苷酸序列以及SEQ ID NO： 变体和片段。

公开(公告)号：EP1947184A2

公开(公告)日：2008-07-23

申请号：EP08005377.0

申请日：2004-02-20

申请人： Athenix Corporation

发明人： Carozzi, Nadine ,  Hargiss, Tracy ,  Koziel, Michael G. ,  Duck, Nicholas, B. ,  Carr, Brian

IPC分类号： C12N15/32 ,  C12N15/82 ,  C12N5/14 ,  C12N15/63 ,  A01H1/00 ,  C07K14/325 ,  C07K16/12 ,  A01N63/00

CPC分类号： C12N15/8286 ,  C07K14/325 ,  Y02A40/162

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin and delta-endotoxin-associated polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin and delta-endotoxin-associated nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequences shown in SEQ ID NOS: 3, 5, 7, 9, 11, 14, 16, 18, 20, 22, 24, 27, and 29, and the nucleotide sequences set forth in SEQ ID NOS: 1, 2, 4, 6, 8, 10, 12, 13, 15, 17, 19, 21, 23, 25, 26, and 28, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素和δ-内毒素相关多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素和δ-内毒素相关的核酸分子。 另外，包含对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：3,5,7,9,11,14,16,18,20,22,24,27， 和29，以及SEQ ID NOS：1,2,4,6,8,10,12,13,15,17,19,21,23,25,26和28中所示的核苷酸序列，以及 变体和片段。