摘要:
The present invention provides an apparatus for producing patterns on a surface of a substrate. The apparatus includes a rigid support member such as a rigid tubular structure having first and second opposed ends and a fluid flow passageway extending therethrough. A printing stamp is attached at one of the opposed ends of the rigid support member. The printing stamp has a flexible diaphragm portion which has an outer surface which is coated with one or more materials in a pre-selected pattern. A pneumatic pressurizing mechanism communicating with an inner surface of the flexible diaphragm portion through the fluid flow passageway is used to bias the flexible diaphragm portion outwardly into intimate and substantially uniform contact with the surface of the substrate for transferring the pre-selected pattern onto said surface. The rigid tubular supports are attached to a robotic positioning mechanism for providing control of positioning of the stamp relative to the substrate surface.
摘要:
Fabrication of surfaces patterned with chemical crosslinkers for solution-phase immobilization of probe molecules and their use in diffraction-based sensing. In one embodiment of the invention, a chemical crosslinker, X1 - R1 -Y1, is deposited on areas of the substrate surface that defines a pattern and allowed to react with the surface for a sufficient period of time to attain the desired density of covalently linked crosslinkers on the surface. The reaction between the crosslinker X1 - R1 - Y1 and the surface can be accelerated using known techniques such as heating, microwave irradiation, sonication, etc, to achieve the desired density in less time. In another embodiment of the invention, two or more other types of cross-linkers may also be laid down in patterns on the surface to detect for two or more other types of molecules in solution.
摘要:
The present invention relates to a method and apparatus for detecting analytes in a medium, and more particularly the present invention relates to an assay based on light diffraction which appears or changes upon the binding of analytes to their specific receptors laid out in patterns on a substrate, which has high sensitivity due to the appropriate choice of such patterns. The present invention is based on the principle that the pattern of recognition elements, which gives rise to the diffraction of the incident light in a diffraction-based assay, can be chosen in such a way so as to facilitate detection, and to enhance the signal to be detected compared to known gratings such as parallel straight lines. In one aspect the substrate itself has a surface topography designed to enhance the diffraction pattern signals. In another aspect the substrate is a diffractive optic element having the analyte-specific receptors affixed to the optic element. In another aspect the diffractive optic element is used as a master stamp for producing patterns of analyte-specific receptors which give the signal enhancements.