摘要:
The invention relates to a method for the biotransformation of carotenoids using enzymes having a cytochrome P450 monooxygenase activity, especially monooxygenases of thermophilic bacteria, especially the genus Thermus sp.
摘要:
The invention relates to novel cytochrome P450 monooxygenases consisting of thermophilic bacteria, especially the species Thermus sp., nucleotide sequences coding for the same, the recombinant production of said monooxygenases and the use thereof for the microbiological oxidation of organic compounds.
摘要:
The invention relates to novel cytochrome P450 monooxygenases comprising a modified substrate specificity, to nucleotide sequences which code therefor, to expression constructs and vectors containing these sequences, and to microorganisms transformed therewith. The invention also relates to methods for microbiologically oxidizing different organic substrates, such as methods for producing indigo and indirubin.
摘要:
The invention relates to modified cytochrome P450 monooxygenases comprising a modified substrate profile, to nucleic acid sequences that code therefor, to expression constructs and vectors, and to recombinant microorganisms which contain these vectors. The invention also relates to methods for microbiologically producing terminally or subterminally hydroxylated aliphatic carboxylic acids.
摘要:
The invention relates to a method for producing a hydroxylation catalyst by: i) incorporating a cytochrome P450 monooxygenase in a sol-gel matrix; ii) incorporating an enzymatic NADPH regeneration system in a sol-gel matrix, and; combining both constituents i) and ii) provided that they had not already been mixed together before incorporation.
摘要:
The invention relates to a method for producing a normalized gene library from nucleic acid extracts of soil samples and to the genetic structures and vectors used therein. The invention further relates to the use of the normalized gene library for screening genes that encode novel biocatalysts from soil samples.
摘要:
The invention relates to modified cytochrome P450 monooxygenases comprising a modified substrate profile, to nucleic acid sequences that code therefor, to expression constructs and vectors, and to recombinant microorganisms which contain these vectors. The invention also relates to methods for microbiologically producing terminally or subterminally hydroxylated aliphatic carboxylic acids.
摘要:
The invention relates to a method for producing a normalized gene library from nucleic acid extracts of soil samples and to the genetic structures and vectors used therein. The invention further relates to the use of the normalized gene library for screening genes that encode novel biocatalysts from soil samples.
摘要:
The invention relates to a method for producing a hydroxylation catalyst by: i) incorporating a cytochrome P450 monooxygenase in a sol-gel matrix; ii) incorporating an enzymatic NADPH regeneration system in a sol-gel matrix, and; combining both constituents i) and ii) provided that they had not already been mixed together before incorporation.