公开(公告)号：EP1162461B1

公开(公告)日：2008-04-09

申请号：EP01112904.6

申请日：2001-06-05

申请人： BAYER CORPORATION

发明人： Malin, Michael J. ,  Shapiro, Phyllis

IPC分类号： G01N33/72 ,  G01N33/49

CPC分类号： G01N33/721 ,  G01N33/726

公开(公告)号：EP0743519B1

公开(公告)日：2002-08-28

申请号：EP96106957.2

申请日：1996-05-03

申请人： Bayer Corporation

发明人： Malin, Michael J. ,  Shapiro, Phyllis ,  Cremins, John F.

IPC分类号： G01N33/50 ,  C12Q1/28 ,  G01N1/30

CPC分类号： G01N33/5094 ,  Y10T436/106664 ,  Y10T436/107497

公开(公告)号：EP1162461A2

公开(公告)日：2001-12-12

申请号：EP01112904.6

申请日：2001-06-05

申请人： Bayer Corporation

发明人： Malin, Michael J. ,  Shapiro, Phyllis

IPC分类号： G01N33/72

CPC分类号： G01N33/721 ,  G01N33/726

摘要： The invention provides a new method and system for detecting and monitoring extracellular or exogenously added hemoglobin, i.e., a cell-free hemoglobin substitute or derivative, in a blood, plasma, or serum sample of an individual, particularly a whole blood sample. The invention further describes the use of automated hematlogy analyzers to determine and quantify at the same time the concentration of total, cellular and exogenous hemoglobin in a blood, plasma, or serum sample, and is particularly advantageous for medical use during or after patient trauma or surgery, as well as for monitoring hemoglobin levels during patient recovery.

摘要翻译： 本发明提供了用于检测和监测细胞外或外源添加的血红蛋白，即，血液，血浆，或血清的个体，特别是全血样品的样品中的无细胞的血红蛋白代用品或衍生物的新的方法和系统。 本发明还描述了使用自动hematlogy分析仪确定的矿山，并在同一时间定量总的，蜂窝式和外源性血红蛋白的血液，血浆或血清样品中的浓度，并且是用于医疗用途特别有利的过程中或患者的创伤或后 手术，以及为病人恢复过程中监测血红蛋白水平。

公开(公告)号：EP0743519A2

公开(公告)日：1996-11-20

申请号：EP96106957.2

申请日：1996-05-03

申请人： Bayer Corporation

发明人： Malin, Michael J. ,  Shapiro, Phyllis ,  Cremins, John F.

IPC分类号： G01N33/50 ,  C12Q1/28 ,  G01N1/30

CPC分类号： G01N33/5094 ,  Y10T436/106664 ,  Y10T436/107497

摘要： The present invention provides an improved reagent composition and method to perform white blood cell differential counting and subpopulation analysis using both fresh and aged blood samples with accuracy and precision. The invention is particularly applicable for the analysis of aged blood samples that have been stored at room temperature for over a day, thereby allowing accurate and useful information to be obtained from samples that are normally considered to be suboptimal. The improved reagent composition and method are particularly related to the peroxidase method of white blood cell differential determinations. One aspect of the invention includes an improved aqueous reagent composition for carrying out the peroxidase method of differential counting. Another aspect includes the use of a rinse cycle and rinse solution devoid of hemolytic surfactant to alleviate the adverse effects of rinse carryover and to streamline and economize the analytical process, particularly when the analyses are performed on automated hematology analyzers and flow cytometry systems. The composition and method of the invention provide clinically useful data for the differential analysis of whole blood samples.

摘要翻译： 本发明提供了改进的试剂组合物和方法，使用新鲜的和老化的血液样品与准确度和精度执行白细胞差分计数和亚群分析。 本发明特别适用于年龄在一天thathave被存储在室温下的血液样品的分析，从而允许获得精确和有用的信息，从样品并通常被认为是次优的。 改进的试剂组合物和方法特别相关的白细胞差测定的过氧化物酶方法。 本发明的一个方面包括在用于执行差分计数的过氧化物酶方法改进wässrige试剂组合物。 另一个方面包括使用漂洗循环的漂洗和无液溶血的表面活性剂以减轻漂洗残留的不利影响，并简化和节约分析过程，特别是当分析被执行在自动血液分析仪和流式细胞仪系统。 本发明的组合物和方法提供了全血样品的差异分析临床上有用的数据。

公开(公告)号：EP1162461A3

公开(公告)日：2002-03-06

申请号：EP01112904.6

申请日：2001-06-05

申请人： Bayer Corporation

发明人： Malin, Michael J. ,  Shapiro, Phyllis

IPC分类号： G01N33/72 ,  G01N33/49

CPC分类号： G01N33/721 ,  G01N33/726

摘要： The invention provides a new method and system for detecting and monitoring extracellular or exogenously added hemoglobin, i.e., a cell-free hemoglobin substitute or derivative, in a blood, plasma, or serum sample of an individual, particularly a whole blood sample. The invention further describes the use of automated hematlogy analyzers to determine and quantify at the same time the concentration of total, cellular and exogenous hemoglobin in a blood, plasma, or serum sample, and is particularly advantageous for medical use during or after patient trauma or surgery, as well as for monitoring hemoglobin levels during patient recovery.

公开(公告)号：EP0743356B1

公开(公告)日：2002-10-09

申请号：EP96106953.1

申请日：1996-05-03

申请人： Bayer Corporation

发明人： Malin, Michael J. ,  Shapiro, Phyllis

IPC分类号： C11D1/722 ,  C11D3/37

CPC分类号： C11D3/3707 ,  C11D1/722 ,  Y10S435/962 ,  Y10T436/10 ,  Y10T436/114998

公开(公告)号：EP0743356A1

公开(公告)日：1996-11-20

申请号：EP96106953.1

申请日：1996-05-03

申请人： Bayer Corporation

发明人： Malin, Michael J. ,  Shapiro, Phyllis

IPC分类号： C11D1/722 ,  C11D3/37

CPC分类号： C11D3/3707 ,  C11D1/722 ,  Y10S435/962 ,  Y10T436/10 ,  Y10T436/114998

摘要： The present invention provides a heretofore unknown use for a aqueous reagent composition that serves as a universal rinse for performing and/or improving a variety of hematological analyses on automated analyzers. The universal rinse reagent comprises a phosphate buffer to maintain the rinse solution pH at from about 7.0 to about 7.6; a nonhemolytic nonionic surfactant, such as a Pluronic; an alkali metal salt, such as NaCl; antimicrobial and anti-oxidant compounds; and has an osmolality of about 285 to 305 mOsmol/kg. The universal rinse reagent composition is highly suitable for use in the rinse phases or cycles of all types of blood cell analysis methods and processes performed on semi- and fully-automated systems. The invention allows the replacement of mutiple and specific rinse solutions with the disclosed universal rinse to obtain accurate and acceptable results, independent of the types of blood cell analyses that are performed. The universal rinse is most particularly useful for automated systems having intricate hardware and a number of different input and output channels. The universal rinse composition serves to economize, streamline, and simplify the design and operation of such systems.

摘要翻译： 本发明提供了用于水性试剂组合物的迄今为止未知的用途，其用作在自动分析仪上进行和/或改进各种血液学分析的通用冲洗液。 通用冲洗试剂包含磷酸盐缓冲液，以将漂洗溶液的pH保持在约7.0至约7.6; 非溶解性非离子表面活性剂，例如Pluronic; 碱金属盐，如NaCl; 抗菌和抗氧化剂; 并且具有约285至305mOsmol / kg的重量克分子渗透压浓度。 通用冲洗试剂组合物非常适合用于在半自动和全自动化系统中进行的所有类型血细胞分析方法和过程的漂洗阶段或循环。 本发明允许用所公开的通用冲洗液替换多种和特定的漂洗溶液，以获得准确和可接受的结果，而与执行的血细胞分析的类型无关。 通用冲洗对于具有复杂硬件和多个不同输入和输出通道的自动化系统最为特别有用。 通用冲洗组合物用于节约，简化和简化这些系统的设计和操作。