公开(公告)号：EP0693925A1

公开(公告)日：1996-01-31

申请号：EP94914102.0

申请日：1994-04-08

申请人： BIO-TECHNOLOGY GENERAL CORP. ,  YISSUM RESEARCH DEVELOPMENT COMPANY OF THE HEBREW UNIVERSITY OF JERUSALEM

发明人： WERBER, Moshe M. ,  ZEELON, Elisha P. ,  LEVANON, Avigdor ,  GUY, Rachel ,  GOLDLUST, Arie ,  RIGBI, Meir ,  PANET, Amos ,  FISCHER, Meir

IPC分类号： C12N15 ,  A61K38 ,  A61P7 ,  A61P31 ,  A61P43 ,  C07K1 ,  C07K14 ,  C07K16 ,  C12N1 ,  C12N9 ,  C12P21 ,  C12R1

CPC分类号： C07K14/815 ,  A61K38/00 ,  C07K16/38

摘要： The present invention provides a recombinant polypeptide having the amino acid sequence X-tyr?26 - gly110¿ where tyr?26 - gly110¿ is identical to the sequence shown in Figure 10, and wherein X is methionine or absent and wherein asn72 may be substituted by pro. The invention further provides a method of producing the polypeptide which comprises transforming a host cell with an expression plasmid encoding the polypeptide, culturing the transformed host cell so that the cell produces the polypeptide encoded by the plasmid, and a method of recovering the polypeptide so produced.

公开(公告)号：EP0693925B1

公开(公告)日：2002-02-13

申请号：EP94914102.2

申请日：1994-04-08

申请人： BIO-TECHNOLOGY GENERAL CORPORATION ,  YISSUM RESEARCH DEVELOPMENT COMPANY OF THE HEBREW UNIVERSITY OF JERUSALEM

发明人： WERBER, Moshe M. ,  ZEELON, Elisha P. ,  LEVANON, Avigdor ,  GUY, Rachel ,  GOLDLUST, Arie ,  RIGBI, Meir ,  PANET, Amos ,  FISCHER, Meir

IPC分类号： A61K31/00 ,  A61K38/00 ,  C07K1/00 ,  C07K14/00 ,  C07H17/00 ,  C12N15/00

CPC分类号： C07K14/815 ,  A61K38/00 ,  C07K16/38

摘要： The present invention provides a recombinant polypeptide having the amino acid sequence X-tyr - gly where tyr - gly is identical to the sequence shown in Figure 10, and wherein X is methionine or absent and wherein asn may be substituted by pro. The invention further provides a method of producing the polypeptide which comprises transforming a host cell with an expression plasmid encoding the polypeptide, culturing the transformed host cell so that the cell produces the polypeptide encoded by the plasmid, and a method of recovering the polypeptide so produced.

公开(公告)号：EP0696198A1

公开(公告)日：1996-02-14

申请号：EP94914087.0

申请日：1994-04-08

申请人： BIO-TECHNOLOGY GENERAL CORP.

发明人： ZEELON, Elisha P. ,  WERBER, Moshe M. ,  LEVANON, Avigdor

IPC分类号： C12N1 ,  C07K14 ,  A61K38 ,  C12R1

CPC分类号： C07K14/81 ,  A61K38/00

摘要： A novel polypeptide derived from the leech Hirudo medicinalis was found to be able to inhibit Factor Xa thereby reducing the extent of blood coagulation. The polypeptide is useful in treating conditions of excessive blood coagulation. A recombinant organism containing cDNA encoding the polypeptide has been deposited in the ATCC under Accession No. 69134. Recombinant microorganisms able to produce the polypeptide have been deposited in the ATCC under Accession Nos. 69135, 69137 and 69269.