公开(公告)号：EP0693925B1

公开(公告)日：2002-02-13

申请号：EP94914102.2

申请日：1994-04-08

申请人： BIO-TECHNOLOGY GENERAL CORPORATION ,  YISSUM RESEARCH DEVELOPMENT COMPANY OF THE HEBREW UNIVERSITY OF JERUSALEM

发明人： WERBER, Moshe M. ,  ZEELON, Elisha P. ,  LEVANON, Avigdor ,  GUY, Rachel ,  GOLDLUST, Arie ,  RIGBI, Meir ,  PANET, Amos ,  FISCHER, Meir

IPC分类号： A61K31/00 ,  A61K38/00 ,  C07K1/00 ,  C07K14/00 ,  C07H17/00 ,  C12N15/00

CPC分类号： C07K14/815 ,  A61K38/00 ,  C07K16/38

摘要： The present invention provides a recombinant polypeptide having the amino acid sequence X-tyr - gly where tyr - gly is identical to the sequence shown in Figure 10, and wherein X is methionine or absent and wherein asn may be substituted by pro. The invention further provides a method of producing the polypeptide which comprises transforming a host cell with an expression plasmid encoding the polypeptide, culturing the transformed host cell so that the cell produces the polypeptide encoded by the plasmid, and a method of recovering the polypeptide so produced.

公开(公告)号：EP0693925A1

公开(公告)日：1996-01-31

申请号：EP94914102.0

申请日：1994-04-08

申请人： BIO-TECHNOLOGY GENERAL CORP. ,  YISSUM RESEARCH DEVELOPMENT COMPANY OF THE HEBREW UNIVERSITY OF JERUSALEM

发明人： WERBER, Moshe M. ,  ZEELON, Elisha P. ,  LEVANON, Avigdor ,  GUY, Rachel ,  GOLDLUST, Arie ,  RIGBI, Meir ,  PANET, Amos ,  FISCHER, Meir

IPC分类号： C12N15 ,  A61K38 ,  A61P7 ,  A61P31 ,  A61P43 ,  C07K1 ,  C07K14 ,  C07K16 ,  C12N1 ,  C12N9 ,  C12P21 ,  C12R1

CPC分类号： C07K14/815 ,  A61K38/00 ,  C07K16/38

摘要： The present invention provides a recombinant polypeptide having the amino acid sequence X-tyr?26 - gly110¿ where tyr?26 - gly110¿ is identical to the sequence shown in Figure 10, and wherein X is methionine or absent and wherein asn72 may be substituted by pro. The invention further provides a method of producing the polypeptide which comprises transforming a host cell with an expression plasmid encoding the polypeptide, culturing the transformed host cell so that the cell produces the polypeptide encoded by the plasmid, and a method of recovering the polypeptide so produced.

公开(公告)号：EP0651799B1

公开(公告)日：1999-08-18

申请号：EP91911888.5

申请日：1991-05-21

申请人： BIO-TECHNOLOGY GENERAL CORPORATION

发明人： VOGEL, Tikva ,  LEVANON, Avigdor ,  WERBER, Moshe ,  GUY, Rachel ,  PANET, Amos ,  HARTMAN, Jacob ,  SHAKED, Hadassa

IPC分类号： C12N15/12 ,  C12N15/62 ,  C07K14/435 ,  C12N9/70 ,  C12N9/72 ,  A61K51/00 ,  G01N33/68 ,  C07K1/00 ,  A61K38/00

CPC分类号： A61K49/0008 ,  A61K38/00 ,  A61K47/6415 ,  A61K49/0002 ,  A61K49/0004 ,  A61K51/082 ,  A61K51/088 ,  A61K2123/00 ,  A61M25/0045 ,  C07K1/1133 ,  C07K7/06 ,  C07K14/3153 ,  C07K14/78 ,  C07K19/00 ,  C07K2319/00 ,  C07K2319/33

摘要： This invention provides an imaging agent which comprises a polypeptide labeled with an imageable marker, such polypeptide having an amino acid sequence substantially present in the fibrin binding domain of naturally-occuring human fibronectin and being capable of binding to fibrin. The invention further provides a method wherein the imaging agent is used for imaging a fibrin-containing substance, i.e., a thrombus or atherosclerotic plaque. Further provided are plasmids for expression of polypeptides having an amino acid sequence substantially present in the fibrin binding domain of naturally-occuring human fibronectin and being capable of binding to fibrin, hosts containing these plasmids, methods of producing the polypeptides, methods of treatment using the polypeptides, and methods of recovering, refolding and reoxidizing the polypeptides. The invention also provides for purified polypeptides substantially free of other substances of human origin which have an amino acid sequence substantially present in the fibrin binding domain of naturally-occuring human fibronectin and which are capable of binding to fibrin.

公开(公告)号：EP1353937A2

公开(公告)日：2003-10-22

申请号：EP01994329.9

申请日：2001-12-31

申请人： BIO-TECHNOLOGY GENERAL CORPORATION

发明人： HAGAI, Yocheved ,  LAZAROVITS, Janette ,  GUY, Rachel ,  LIPSCHITZ, Orly ,  SZANTON, Esther ,  LEVANON, Avigdor ,  PLAKSIN, Daniel ,  PERETZ, Tuvia

IPC分类号： C07H21/04 ,  C07K5/00 ,  A61K39/395 ,  C12N15/00 ,  G01N33/53

CPC分类号： C07K14/472 ,  A61K38/00

摘要： The present invention is directed to a peptide or polypeptide comprising an Fv molecule, a construct thereof, a fragment of either, or a construct of a fragment having enhanced binding characteristics so as to bind selectively and/or specifically to a target cell in favor of other cells, wherein the binding selectivity or specificity is primarily determined by a first hypervariable region, and wherein the Fv is a scFv or a dsFv, and optionally having one or more tags. The enhanced binding is directed to a substantially exposed and/or over-expressed binding site on or in a target comprising a cell in favor of other cells on or in which the binding site is not substantially available and/or expressed. The invention is further directed to a method for isolating such peptides and polypeptides from a phage display library and to the nucleic acid molecules encoding them. The invention provides for a pharmaceutical composition comprising the peptide or polypeptide and kits for diagnosis and treatment of disease, specifically cancer, most specifically acute myeloid leukemia.

公开(公告)号：EP0651799A1

公开(公告)日：1995-05-10

申请号：EP91911888.0

申请日：1991-05-21

申请人： BIO-TECHNOLOGY GENERAL CORPORATION

发明人： VOGEL, Tikva ,  LEVANON, Avigdor ,  WERBER, Moshe ,  GUY, Rachel ,  PANET, Amos ,  HARTMAN, Jacob ,  SHAKED, Hadassa

IPC分类号： A61K38 ,  A61K47 ,  A61K49 ,  A61K51 ,  A61M25 ,  C07K1 ,  C07K7 ,  C07K14 ,  C07K19 ,  C12N1 ,  C12N9 ,  C12N15 ,  C12P21 ,  C12Q1 ,  C12R1

CPC分类号： A61K49/0008 ,  A61K38/00 ,  A61K47/6415 ,  A61K49/0002 ,  A61K49/0004 ,  A61K51/082 ,  A61K51/088 ,  A61K2123/00 ,  A61M25/0045 ,  C07K1/1133 ,  C07K7/06 ,  C07K14/3153 ,  C07K14/78 ,  C07K19/00 ,  C07K2319/00 ,  C07K2319/33

摘要： This invention provides an imaging agent which comprises a polypeptide labeled with an imageable marker, such polypeptide having an amino acid sequence substantially present in the fibrin binding domain of naturally-occuring human fibronectin and being capable of binding to fibrin. The invention further provides a method wherein the imaging agent is used for imaging a fibrin-containing substance, i.e., a thrombus or atherosclerotic plaque. Further provided are plasmids for expression of polypeptides having an amino acid sequence substantially present in the fibrin binding domain of naturally-occuring human fibronectin and being capable of binding to fibrin, hosts containing these plasmids, methods of producing the polypeptides, methods of treatment using the polypeptides, and methods of recovering, refolding and reoxidizing the polypeptides. The invention also provides for purified polypeptides substantially free of other substances of human origin which have an amino acid sequence substantially present in the fibrin binding domain of naturally-occuring human fibronectin and which are capable of binding to fibrin.