公开(公告)号：EP1625209B1

公开(公告)日：2013-07-17

申请号：EP04775946.9

申请日：2004-05-06

申请人： BIOGEN IDEC HEMOPHILIA INC.

发明人： PETERS, Robert, T. ,  MEZO, Adam, R. ,  RIVERA, Daniel, S. ,  BITONTI, Alan, J. ,  STATTEL, James, M. ,  LOW, Susan, C.

IPC分类号： C07K14/475 ,  C07K14/505 ,  C07K14/555 ,  C07K14/56 ,  C07K14/565 ,  C07K14/745 ,  C07K16/00 ,  C12N9/64

CPC分类号： C07K14/755 ,  A61K38/00 ,  A61K47/60 ,  A61K47/642 ,  A61K47/68 ,  A61K47/6803 ,  A61K47/6811 ,  A61K47/6813 ,  A61K47/6835 ,  C07K14/475 ,  C07K14/505 ,  C07K14/555 ,  C07K14/56 ,  C07K14/565 ,  C07K14/59 ,  C07K14/61 ,  C07K14/70503 ,  C07K14/745 ,  C07K16/00 ,  C07K2317/52 ,  C07K2319/00 ,  C07K2319/30 ,  C12N9/6437 ,  C12N9/644 ,  C12N9/647 ,  C12N9/96 ,  C12Y304/21021 ,  C12Y304/21022

摘要： A chimeric protein is synthesized by a) recombinantly expressing a fusion protein comprising at least a portion of an immunoglobulin constant region and a splicing protein capable of forming a C terminus thioester on the portion of an immunoglobulin constant region; b) adding a thiol cofactor to the fusion protein of a); and c) adding at least one biologically active molecule having an N terminal cysteine.