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公开(公告)号：EP0988378B9
公开(公告)日：2010-08-04
申请号：EP98901380.0
申请日：1998-01-26
CPC分类号： C12N15/102 , C07K16/00 , C07K16/44 , C07K2317/622 , C07K2317/92 , C12N15/1027 , C12N15/1031 , C12N15/1058 , C12N15/1086 , Y10T436/143333
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公开(公告)号：EP0990044A1
公开(公告)日：2000-04-05
申请号：EP98930871.3
申请日：1998-06-16
发明人： BORREBAECK, Carl, Arne, Krister , SÖDERLIND, Ulf, Hans, Eskil , OTTOSSON, Rebecka, Ingrid, Camilla
IPC分类号： C12Q1/68
CPC分类号： C40B40/02 , C12N15/1027 , C12N15/1037 , C12Q1/6811 , C12Q1/6858 , C12Q2521/319
摘要： The present invention relates to a method for in vitro evolution of protein function. In particular, the method relates to the shuffling of nucleotide segments obtained from exonuclease digestion. The present inventors have shown that polynucleotide fragments derived from a parent polynucleotide sequence digested with an exonuclease can be combined to generate a polynucleotide sequence which encodes for a polypeptide having desired characteristics. This method may be usefully applied to the generation of new antibodies or parts thereof having modified characteristics as compared to the parent antibody.
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公开(公告)号：EP0988378B1
公开(公告)日：2003-11-26
申请号：EP98901380.0
申请日：1998-01-26
CPC分类号： C12N15/102 , C07K16/00 , C07K16/44 , C07K2317/622 , C07K2317/92 , C12N15/1027 , C12N15/1031 , C12N15/1058 , C12N15/1086 , Y10T436/143333
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公开(公告)号：EP0988378B2
公开(公告)日：2009-11-18
申请号：EP98901380.0
申请日：1998-01-26
CPC分类号： C12N15/102 , C07K16/00 , C07K16/44 , C07K2317/622 , C07K2317/92 , C12N15/1027 , C12N15/1031 , C12N15/1058 , C12N15/1086 , Y10T436/143333
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公开(公告)号：EP0988378A1
公开(公告)日：2000-03-29
申请号：EP98901380.0
申请日：1998-01-26
CPC分类号： C12N15/102 , C07K16/00 , C07K16/44 , C07K2317/622 , C07K2317/92 , C12N15/1027 , C12N15/1031 , C12N15/1058 , C12N15/1086 , Y10T436/143333
摘要： The present invention relates to a method for in vitro creation of molecular libraries evolution of protein function. Particularly, it relates to variability and modification of protein function by shuffling polynucleotide sequence segments. A protein of desired characteristics can be obtained by incorporating variant peptide regions (variant motifs) into defined peptide regions (scaffold sequence). The variant motifs can be obtained from parent DNA which has been subjected to mutagenesis to create a plurality of differently mutated derivatives thereof or they can be obtained from in vivo sequences. These variant motifs can then be incorporated into a scaffold sequence and the resulting coded protein screened for desired characteristics. This method is ideally used for obtaining antibodies with desired characteristics by isolating individual CDR DNA sequences and incorporating them into a scaffold which may, for example, be from a totally different antibody.
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