专利检索 ap:("BIONEBRASKA, INC.") AND inv:"STRYDOM, Daniel, J." 第 1 页

1.

发明公开
ENZYMATIC AMIDATION OF PEPTIDES 审中-公开
标题翻译：肽酶法酰胺化

公开(公告)号：EP1127155A1

公开(公告)日：2001-08-29

申请号：EP99956920.5

申请日：1999-11-05

申请人： BIONEBRASKA, INC.

发明人： DORMADY, Dan , STOUT, Jay, S. , STRYDOM, Daniel, J. , HOLMQUIST, Barton , WAGNER, Fred, W.

IPC分类号： C12P21/06

CPC分类号： C07K14/605 , C12P21/06

摘要： The invention provides a method of producing a polypeptide having a C-terminal α-carboxamide group. It particularly concerns an enzymatic modification of selected substrate polypeptides which result in cleavage of the substrate polypeptide to form a product peptide with a C-terminal arginine residue having an α-carboxamide group (C-terminal 'Arg-NH2'). The method includes contacting an aqueous-based solution including (i) ammonia reagent and (ii) the substrate polypeptide with (iii) clostripain.