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    PROCESS AND APPARATUS FOR QUANTIFYING NUCLEIC ACID IN A SAMPLE
    2.
    发明公开
    PROCESS AND APPARATUS FOR QUANTIFYING NUCLEIC ACID IN A SAMPLE 有权
    VERFAHREN UND VORRICHTUNG ZUR QUANTIFIZIERUNG VONNUKLEINSÄURENIN EINER PROBE

    公开(公告)号：EP2726633A2

    公开(公告)日：2014-05-07

    申请号：EP12804520.0

    申请日：2012-07-02

    申请人： Bioquanta Sa ,  Bioquanta Corp. ,  Assistance Publique Hôpitaux De Paris

    发明人： CONTI, Marc ,  LORIC, Sylvain ,  MANIVET, Philippe ,  DELACOTTE, Nicolas ,  KEUMEUGNI KWEMO, Carlosse ,  SALIOU BAH, Mamadou

    IPC分类号： C12Q1/68 ,  G01N33/53

    CPC分类号： C12Q1/6806 ,  C12Q1/68 ,  C12Q2537/125 ,  C12Q2545/113 ,  C12Q2563/173 ,  C12Q2522/101 ,  C12Q2537/161 ,  C12Q2545/114

    摘要： In one aspect, the present invention concerns methods and kits for quantifying a target nucleic acid in a sample. In embodiments, a method comprises sequestrating the target nucleic acid by one or more interactors to form a sequestered conjugate that will be further labeled to form a labeled conjugate. The signal produced by the labeled conjugate is measured and correlated to the amount of the target nucleic acid. In another aspect, the present invention concerns a method for quantifying a target nucleic acid in a sample by a reaction of the nucleic acids in the sample with a nucleic acid interactor or set of interactors to form a conjugate. The conjugate is then sequestered from the rest of the sample with a molecule bound to a support, to form a sequestered conjugate. The sequestered conjugate is labeled to form a labeled conjugate. The signal produced by the labeled conjugate is measured and correlated to the amount of the target nucleic acid. In some embodiments, the target nucleic acid is cell free nucleic acids. In embodiments, the interactor binds to nucleic acids in the sample in a nonsequence specific manner.

    摘要翻译： 一方面，本发明涉及用于定量样品中靶核酸的方法和试剂盒。 在实施方案中，一种方法包括通过一种或多种相互作用体螯合靶核酸以形成将被进一步标记以形成标记的缀合物的螯合缀合物。 测量由标记的缀合物产生的信号并与靶核酸的量相关。 在另一方面，本发明涉及通过样品中的核酸与核酸相互作用者或一组相互作用体的反应来定量样品中的靶核酸以形成缀合物的方法。 然后将缀合物与其余的样品以分子结合到载体上形成螯合偶联物。 将螯合的缀合物标记以形成标记的缀合物。 测量由标记的缀合物产生的信号并与靶核酸的量相关。 在一些实施方案中，靶核酸是无细胞核酸。 在实施方案中，相互作用者以非序列特异性方式结合样品中的核酸。