公开(公告)号：EP2726633A2

公开(公告)日：2014-05-07

申请号：EP12804520.0

申请日：2012-07-02

申请人： Bioquanta Sa ,  Bioquanta Corp. ,  Assistance Publique Hôpitaux De Paris

发明人： CONTI, Marc ,  LORIC, Sylvain ,  MANIVET, Philippe ,  DELACOTTE, Nicolas ,  KEUMEUGNI KWEMO, Carlosse ,  SALIOU BAH, Mamadou

IPC分类号： C12Q1/68 ,  G01N33/53

CPC分类号： C12Q1/6806 ,  C12Q1/68 ,  C12Q2537/125 ,  C12Q2545/113 ,  C12Q2563/173 ,  C12Q2522/101 ,  C12Q2537/161 ,  C12Q2545/114

摘要： In one aspect, the present invention concerns methods and kits for quantifying a target nucleic acid in a sample. In embodiments, a method comprises sequestrating the target nucleic acid by one or more interactors to form a sequestered conjugate that will be further labeled to form a labeled conjugate. The signal produced by the labeled conjugate is measured and correlated to the amount of the target nucleic acid. In another aspect, the present invention concerns a method for quantifying a target nucleic acid in a sample by a reaction of the nucleic acids in the sample with a nucleic acid interactor or set of interactors to form a conjugate. The conjugate is then sequestered from the rest of the sample with a molecule bound to a support, to form a sequestered conjugate. The sequestered conjugate is labeled to form a labeled conjugate. The signal produced by the labeled conjugate is measured and correlated to the amount of the target nucleic acid. In some embodiments, the target nucleic acid is cell free nucleic acids. In embodiments, the interactor binds to nucleic acids in the sample in a nonsequence specific manner.

摘要翻译： 一方面，本发明涉及用于定量样品中靶核酸的方法和试剂盒。 在实施方案中，一种方法包括通过一种或多种相互作用体螯合靶核酸以形成将被进一步标记以形成标记的缀合物的螯合缀合物。 测量由标记的缀合物产生的信号并与靶核酸的量相关。 在另一方面，本发明涉及通过样品中的核酸与核酸相互作用者或一组相互作用体的反应来定量样品中的靶核酸以形成缀合物的方法。 然后将缀合物与其余的样品以分子结合到载体上形成螯合偶联物。 将螯合的缀合物标记以形成标记的缀合物。 测量由标记的缀合物产生的信号并与靶核酸的量相关。 在一些实施方案中，靶核酸是无细胞核酸。 在实施方案中，相互作用者以非序列特异性方式结合样品中的核酸。

公开(公告)号：EP2683399A2

公开(公告)日：2014-01-15

申请号：EP12707757.6

申请日：2012-03-07

申请人： Bioquanta ,  Bioquanta Corp. ,  Assistance Publique Hôpitaux De Paris

发明人： CONTI, Marc ,  LORIC, Sylvain ,  MANIVET, Philippe ,  CARADEC, Josselin ,  KEUMEUGNI KWEMO, Carlosse ,  MATAR, Corine ,  REVAUD, Déborah

IPC分类号： A61K38/57 ,  A61P15/08 ,  G01N33/50

CPC分类号： C07K14/8139 ,  A61K38/57 ,  C12N5/0604 ,  C12N2501/734 ,  G01N33/50 ,  G01N33/689 ,  G01N33/6893 ,  G01N2333/8139 ,  G01N2800/367

摘要： The present invention concerns a polypeptide comprising the N-terminal fragment of a cystatin, and/or a cystatin expression inducer for use for the treatment of sterility and/or infertility, and a method of diagnosing and/or predicting sterility and/or infertility of a subject and/or of a couple of subjects, which method comprises measuring the level of at least one cystatin in a biological sample of a male subject and/or measuring the level of at least one cystatin in a biological sample of a female subject, and optionally measuring further the level of at least one cathepsin in a biological sample of said male of female subject.

摘要翻译： 本发明涉及包含半胱氨酸蛋白酶抑制剂的N-末端片段和/或用于治疗无菌和/或不孕症的半胱氨酸蛋白酶抑制剂表达诱导剂的多肽，以及诊断和/或预测不育和/或不孕不育的方法 受试者和/或几个受试者，所述方法包括测量男性受试者的生物样品中至少一种半胱氨酸蛋白酶抑制剂的水平和/或测量女性受试者的生物样品中至少一种半胱氨酸蛋白酶抑制剂的水平。

公开(公告)号：EP2281064A1

公开(公告)日：2011-02-09

申请号：EP09742212.5

申请日：2009-04-10

申请人： Bioquanta ,  Bioquanta Corp.

发明人： JACOB, Alexandre ,  KEUMEUGNI KWEMO, Carlosse ,  LORIC, Sylvain ,  MOUTEREAUX, Stéphane ,  DELACOTTE, Nicolas

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6813 ,  C12Q1/6834 ,  Y10T436/143333 ,  C12Q2563/185 ,  C12Q2565/607 ,  C12Q2565/125 ,  C12Q2563/131

摘要： The present invention relates to a method for labelling a product, to a method for identifying the labelling and to a product labelled by means of the method of the invention. The labelling used in the present invention is based on single-stranded nucleic acids. The labelling method of the present invention comprises a step of addition of a plurality of single-stranded polynucleotides onto or into said product, said plurality of polynucleotides comprising: at least one target polynucleotide comprising a single-stranded polynucleotide of predetermined length and sequence, and decoy polynucleotides which have identical or different predetermined lengths and identical or different predetermined sequences, said decoy polynucleotides having a length or lengths identical to or different from and sequences different from the sequence of said, at least one, target polynucleotide, in which each of the target polynucleotide(s) and decoy polynucleotides does not hybridize with any of the other polynucleotides of said plurality of polynucleotides. The method of the invention makes it possible, for example, to label fragrances, cosmetics, hygiene products, food products, flavourings, extracts of a plant or plants, tobacco, beverages, textiles, leathers, medicaments, powders, varnishes, inks, hydrocarbons, papers, paints, and chemical products and compounds.

公开(公告)号：EP2726633B1

公开(公告)日：2018-01-24

申请号：EP12804520.0

申请日：2012-07-02

申请人： BIOQUANTA SA ,  BIOQUANTA, CORP. ,  ASSISTANCE PUBLIQUE - HOPITAUX DE PARIS

发明人： CONTI, Marc ,  LORIC, Sylvain ,  MANIVET, Philippe ,  DELACOTTE, Nicolas ,  KEUMEUGNI KWEMO, Carlosse ,  SALIOU BAH, Mamadou

IPC分类号： C12Q1/68 ,  G01N33/53

CPC分类号： C12Q1/6806 ,  C12Q1/68 ,  C12Q2537/125 ,  C12Q2545/113 ,  C12Q2563/173 ,  C12Q2522/101 ,  C12Q2537/161 ,  C12Q2545/114

摘要： In one aspect, the present invention concerns methods and kits for quantifying a target nucleic acid in a sample. In embodiments, a method comprises sequestrating the target nucleic acid by one or more interactors to form a sequestered conjugate that will be further labeled to form a labeled conjugate. The signal produced by the labeled conjugate is measured and correlated to the amount of the target nucleic acid. In another aspect, the present invention concerns a method for quantifying a target nucleic acid in a sample by a reaction of the nucleic acids in the sample with a nucleic acid interactor or set of interactors to form a conjugate. The conjugate is then sequestered from the rest of the sample with a molecule bound to a support, to form a sequestered conjugate. The sequestered conjugate is labeled to form a labeled conjugate. The signal produced by the labeled conjugate is measured and correlated to the amount of the target nucleic acid. In some embodiments, the target nucleic acid is cell free nucleic acids. In embodiments, the interactor binds to nucleic acids in the sample in a nonsequence specific manner.