公开(公告)号：EP1774024A4

公开(公告)日：2012-04-04

申请号：EP05763933

申请日：2005-07-01

Applicant: BLUESHIFT BIOTECHNOLOGIES INC

Inventor： COMITA PAUL B ,  SHUMATE CHRISTOPHER B ,  MILLER STEVEN C ,  CROMWELL EVAN F

IPC: G01N21/64 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/543

CPC classification number: G01N33/582 ,  B82Y5/00 ,  B82Y10/00 ,  C12Q1/6816 ,  G01N21/6408 ,  G01N21/6428 ,  G01N21/6445 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6432 ,  G01N2021/6441 ,  G01N2021/6478 ,  G01N2021/6484 ,  G01N2201/067 ,  G01N2201/0697 ,  G01N2201/0833 ,  C12Q2563/107 ,  C12Q2561/119

公开(公告)号：EP1866443A4

公开(公告)日：2009-04-08

申请号：EP06758259

申请日：2006-04-03

Applicant: BLUESHIFT BIOTECHNOLOGIES INC

Inventor： MILLER STEVEN C ,  COMITA PAUL B ,  SHUMATE CHRISTOPHER B ,  CROMWELL EVAN F

IPC: C12Q1/68 ,  G01N33/53

CPC classification number: G01N33/542 ,  G01N2500/00

Abstract: Methods and apparatus are described for detecting specific binding between first and second chemical entities. The first chemical entity in association with a first fluorophore is immobilized. The second chemical entity is allowed to bind with the immobilized first chemical entity. The second chemical entity is or becomes coupled to a second fluorophore, which forms a FRET pair with the first fluorophore. The bound chemical entities are exposed to radiation at an excitation frequency for either the first or the second fluorophore, and polarization anisotropy of a FRET fluorescent signal from the bound chemical entities is measured to detect specific binding between the first and second chemical entities. Techniques are also disclosed for detecting whether a FRET interaction is occurring between a first chemical entity including a donor fluorophore and a second chemical entity including an acceptor fluorophore, using simultaneous anisotropy measurements at the wavelengths of the donor and acceptor fluorophores.