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公开(公告)号：EP2213724B1
公开(公告)日：2012-09-26
申请号：EP10004392.6
申请日：2005-10-12
CPC分类号： C12N5/0043 , C12N5/0682 , C12N2500/46 , C12N2500/74 , C12N2500/76 , C12N2500/92
摘要： The present invention relates to an animal protein-free cell culture medium, comprising at least one polyamine and at least one protein hydrolysate derived from the group consisting of plants and yeast, wherein the polyamine is present in the culture medium in a concentration ranging from 0.5 to 10 mg/L and the protein hydrolysate is present in a concentration ranging from 0.05 % (w/v) to about 1 % (w/v).
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公开(公告)号：EP2218776A1
公开(公告)日：2010-08-18
申请号：EP10004394.2
申请日：2005-10-12
CPC分类号： C12N5/0043 , C12N5/0682 , C12N2500/46 , C12N2500/74 , C12N2500/76 , C12N2500/92
摘要： The present invention relates to an animal protein-free cell culture medium, comprising at least one polyamine and at least one protein hydrolysate derived from the group consisting of plants and yeast, wherein the polyamine is present in the culture medium in a concentration ranging from 2 to 8 mg/L and the protein hydrolysate is present in a concentration ranging from 0.05 % (w/v) to about 0.5 % (w/v).
摘要翻译： 本发明涉及不含动物蛋白的细胞培养基，其包含至少一种多胺和至少一种来源于植物和酵母的蛋白质水解产物，其中所述多胺在培养基中的浓度范围为2 至8mg / L，并且蛋白质水解产物以0.05％（w / v）至约0.5％（w / v）的浓度存在。
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公开(公告)号：EP2213725B1
公开(公告)日：2012-08-22
申请号：EP10004393.4
申请日：2005-10-12
CPC分类号： C12N5/0043 , C12N5/0682 , C12N2500/46 , C12N2500/74 , C12N2500/76 , C12N2500/92
摘要： The present invention relates to an animal protein-free cell culture medium, comprising at least one polyamine and at least one protein hydrolysate derived from the group consisting of plants and yeast, wherein the polyamine is present in the culture medium in a concentration ranging from 0.5 to 10 mg/L and the protein hydrolysate is present in a concentration ranging from 0.05 % (w/v) to about 1 % (w/v).
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公开(公告)号：EP2213725A1
公开(公告)日：2010-08-04
申请号：EP10004393.4
申请日：2005-10-12
CPC分类号： C12N5/0043 , C12N5/0682 , C12N2500/46 , C12N2500/74 , C12N2500/76 , C12N2500/92
摘要： The present invention relates to an animal protein-free cell culture medium, comprising at least one polyamine and at least one protein hydrolysate derived from the group consisting of plants and yeast, wherein the polyamine is present in the culture medium in a concentration ranging from 2 to 5 mg/L and the protein hydrolysate is present in a concentration ranging from 0.05 % (w/v) to about 0.25 % (w/v).
摘要翻译： 本发明涉及一种无动物蛋白的细胞培养基，其包含至少一种多胺和至少一种衍生自植物和酵母的蛋白质水解产物，其中所述多胺以2的浓度存在于培养基中 至5mg / L，蛋白质水解物以0.05％（w / v）至约0.25％（w / v）的浓度存在。
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公开(公告)号：EP2213724A1
公开(公告)日：2010-08-04
申请号：EP10004392.6
申请日：2005-10-12
CPC分类号： C12N5/0043 , C12N5/0682 , C12N2500/46 , C12N2500/74 , C12N2500/76 , C12N2500/92
摘要： The present invention relates to an animal protein-free cell culture medium, comprising putrescine and soy hydrolysate, wherein the putrescine is present in the culture medium in a concentration ranging from 0.5 to 10 mg/L and the soy hydrolysate is present in a concentration ranging from 0.05 % (w/v) to about 5 % (w/v).
摘要翻译： 本发明涉及不含动物蛋白的细胞培养基，其包含腐胺和大豆水解产物，其中腐胺以0.5至10mg / L的浓度存在于培养基中，大豆水解产物以一定浓度存在 从0.05％（w / v）至约5％（w / v）。
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公开(公告)号：EP2213726B1
公开(公告)日：2012-12-05
申请号：EP10004395.9
申请日：2005-10-12
CPC分类号： C12N5/0043 , C12N5/0682 , C12N2500/46 , C12N2500/74 , C12N2500/76 , C12N2500/92
摘要： The present invention relates to an animal protein-free cell culture medium, comprising at least one polyamine and at least one protein hydrolysate derived from the group consisting of plants and yeast, wherein the polyamine is present in the culture medium in a concentration ranging from 0.5 to 10 mg/L and the protein hydrolysate is present in a concentration ranging from 0.05 % (w/v) to about 1 % (w/v).
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公开(公告)号：EP2218776B1
公开(公告)日：2012-08-29
申请号：EP10004394.2
申请日：2005-10-12
CPC分类号： C12N5/0043 , C12N5/0682 , C12N2500/46 , C12N2500/74 , C12N2500/76 , C12N2500/92
摘要： The present invention relates to an animal protein-free cell culture medium, comprising at least one polyamine and at least one protein hydrolysate derived from the group consisting of plants and yeast, wherein the polyamine is present in the culture medium in a concentration ranging from 0.5 to 10 mg/L and the protein hydrolysate is present in a concentration ranging from 0.05 % (w/v) to about 1 % (w/v).
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公开(公告)号：EP2213726A1
公开(公告)日：2010-08-04
申请号：EP10004395.9
申请日：2005-10-12
CPC分类号： C12N5/0043 , C12N5/0682 , C12N2500/46 , C12N2500/74 , C12N2500/76 , C12N2500/92
摘要： The present invention relates to an animal protein-free cell culture medium, comprising at least one polyamine and at least one protein hydrolysate derived from the group consisting of plants and yeast, wherein the polyamine is present in the culture medium in a concentration ranging from 0.5 to 10 mg/L and the protein hydrolysate is present in a concentration ranging from 0.05 % (w/v) to about 1 % (w/v).
摘要翻译： 本发明涉及不含动物蛋白的细胞培养基，其包含至少一种多胺和至少一种来源于植物和酵母的蛋白质水解产物，其中所述多胺在培养基中的浓度范围为0.5 至10mg / L，并且蛋白质水解产物以0.05％（w / v）至约1％（w / v）的浓度存在。
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9.发明公开Method for producing Influenza virus in protein-free cell culture medium 失效蛋白质自由基Zellkulturmedium中的Verfahren zur Herstellung von Influenzaviren
公开(公告)号：EP2290053A1
公开(公告)日：2011-03-02
申请号：EP10179339.6
申请日：1995-11-10
IPC分类号： C12N7/00 , C12N5/00 , A61K39/145 , C12P21/00 , A61K39/12 , C07K14/11 , A61K39/15 , A61K39/245
CPC分类号： C07K14/005 , A61K39/00 , A61K39/12 , A61K39/145 , A61K39/245 , A61K2039/5252 , A61K2039/55505 , C12N7/00 , C12N2500/95 , C12N2710/16634 , C12N2710/16651 , C12N2710/24151 , C12N2720/12351 , C12N2760/16122 , C12N2760/16134 , C12N2760/16151 , C12N2760/16234 , C12N2770/24151 , Y02A50/388 , Y02A50/396 , Y02A50/464
摘要： The present invention includes an approach for producing viruses, such as Influenza, and vaccines derived therefrom as well as recombinant proteins derived from viral vectors, by utilizing vertebrate cells cultured under protein-free conditions. These cells, which include a cellular biomass, show improved capabilities for propagating viruses, and eliminate the need for costly and time-consuming viral passaging and purification. The invention also includes further approaches for enhancing the propagation of virus by employing activating substances, modifying the activation site of viruses and using augmentation loops. Improved approaches for producing viral reassortants also are provided.
摘要翻译： 本发明通过利用在无蛋白质条件下培养的脊椎动物细胞，包括用于产生病毒如流感的方法，以及由其衍生的疫苗以及衍生自病毒载体的重组蛋白质。 这些细胞，其包括细胞生物质，显示出增强的病毒传播能力，并消除对昂贵且耗时的病毒传代和纯化的需要。 本发明还包括通过使用活化物质来增强病毒繁殖的进一步方法，修改病毒的活化位点和使用增强环。 还提供了用于生产病毒重配体的改进方法。
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