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    CULTURE MEDIUM FOR EUKARYOTIC CELLS
    5.
    发明公开
    CULTURE MEDIUM FOR EUKARYOTIC CELLS 审中-公开
    培养基中的真核细胞

    公开(公告)号：EP2823033A1

    公开(公告)日：2015-01-14

    申请号：EP13710911.2

    申请日：2013-03-08

    申请人： Friesland Brands B.V.

    发明人： GUPTA, Abhishek ,  GADELLAA, Mireille Maria ,  MAES, Dominick Yves Willy

    IPC分类号： C12N5/00

    CPC分类号： C12N5/0018 ,  C12N5/0043 ,  C12N2500/30 ,  C12N2500/32 ,  C12N2500/33 ,  C12N2500/34 ,  C12N2500/40 ,  C12N2500/76 ,  C12N2500/92 ,  C12N2501/40 ,  C12N2501/999

    摘要： The invention pertains to the use of (i) one or more C
    2 -C
    6 alpha-hydroxy acids, salts of these acids, esters of these acids and combinations thereof; and (ii) one or more compounds selected from -the group of amino acid derivatives consisting of γ-glutamyl amino acids, pyroglutamyl amino acids, glutamate-containing or proline-containing dipeptides, oxo-aminoacids, homo-amino acids, N-acetyl amino-acids and glycyl-glycine; -phenolic acid derivatives; -linear C
    2 -C
    6 or cyclic C
    6 sugar alcohols; -pyridinic acid derivatives; and -nucleobases and/or nucleotides chosen from uracil, adenine,adenosine 3'- monophosphate (3'-AMP) and adenosine 5'-monophosphate (AMP), as a growth-and production promoting ingredient, in culture media for culturing eukaryotic cells. The invention further pertains to culture media containing these componentsat levels of at least 0.001 mg/l.

    ISOLATED HUMAN CELL, METHOD FOR OBTAINING THE SAME AND THEIR USE
    8.
    发明公开
    ISOLATED HUMAN CELL, METHOD FOR OBTAINING THE SAME AND THEIR USE 有权
    ISOLIERTE MENSCHLICHE ZELLE，VERFAHREN ZUR GEWINNUNG DAVON UND DEREN VERWENDUNG

    公开(公告)号：EP1950285A1

    公开(公告)日：2008-07-30

    申请号：EP06811189.7

    申请日：2006-10-04

    申请人： GenomIdea Inc.

    发明人： YANO, Takahiro ,  NAGASAWA, Tetsuji ,  TEMMA, Akiko ,  SUZUKI, Naho ,  MIYAJI, Kazue

    IPC分类号： C12N5/06 ,  C12N5/02 ,  C12N7/02 ,  C12R1/91

    CPC分类号： C12N5/0693 ,  C12N5/0686 ,  C12N7/00 ,  C12N2500/46 ,  C12N2500/92 ,  C12N2710/10051 ,  C12N2760/18851

    摘要： A novel cell suited for mass production of Hemagglutinating Virus of Japan (HVJ), a method for obtaining the cell and use of the cell are disclosed. The human cell is originated from a transformed human kidney cell line, the doubling time thereof in logarithmic growth phase in suspension culture in a serum-free medium is not more than 40 hours, the cell has a freeze-recovery property, the maximum density of viable cells in suspension culture is not less than 10 6 cells/mL, and HVJ can grow in the cell. The method for obtaining the human cell comprises the steps of suspension-culturing a human transformed kidney cell line in a serum-free medium, and cloning the grown cells; and selecting, from the cloned cells, a cell whose doubling time in logarithmic growth phase in suspension culture in a serum-free medium is not more than 40 hours, which has a freeze-recovery property, whose maximum density of viable cells in suspension culture is not less than 10 6 cells/mL, in which HVJ can grow.

    摘要翻译： 公开了一种适用于批量生产日本血凝病毒（HVJ）的新型细胞，获得细胞的方法和该细胞的用途。 人细胞源自转化的人肾细胞系，其在无血清培养基中的悬浮培养中的对数生长期的倍增时间不超过40小时，细胞具有冷冻恢复性，最大密度 悬浮培养中的活细胞不低于10 6个细胞/ mL，HVJ可以在细胞中生长。 获得人细胞的方法包括以下步骤：在无血清培养基中悬浮培养人转化的肾细胞系，并克隆生长的细胞; 从克隆的细胞中选出在无血清培养基中悬浮培养的对数生长期的倍增时间不超过40小时的细胞，其具有冷冻恢复特性，其悬浮培养物中活细胞的最大密度 不小于10 6个细胞/ mL，其中HVJ可以生长。