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1.发明公开METHODS AND MEANS FOR EXACT REPLACEMENT OF TARGET DNA IN EUKARYOTIC ORGANISMS 有权的方法和手段靶DNA在真核生物EXACT交换
公开(公告)号：EP2160467A1
公开(公告)日：2010-03-10
申请号：EP08759067.5
申请日：2008-06-03
IPC分类号： C12N15/82
CPC分类号： C12N15/8213
摘要： Methods and means are provided for the exact exchange in eukaryotic cells, such as plant cells, of a target DNA sequence for a DNA sequence of interest through homologous recombination, whereby the selectable or screenable marker used during the homologous recombination phase for temporal selection of the gene replacement events can subsequently be removed without leaving a foot-print employing a method for the removal of a selected DNA flanked by two nucleotide sequences in direct repeats.
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公开(公告)号：EP1929017A1
公开(公告)日：2008-06-11
申请号：EP06793498.4
申请日：2006-09-14
申请人： BAYER CROPSCIENCE SA
IPC分类号： C12N15/82
CPC分类号： C12N15/8214 , C07K14/8117 , C12N15/8221 , C12N15/8257
摘要： The present invention relates to constructs and methods for the expression of recombinant proteins in the thylakoid lumen of transplastomic plant cells. Furthermore, the present invention relates to transplastomic plant cells and plants expressing a gene of interest in the thylakoid lumen.
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公开(公告)号：EP1836308A2
公开(公告)日：2007-09-26
申请号：EP06706453.5
申请日：2006-01-03
申请人： BAYER CROPSCIENCE SA
CPC分类号： C12N15/8274 , C12N15/8214 , C12N15/8275
摘要： The present invention relates to transplastomic plants free of the selectable marker gene, to a method of obtaining such plants and to the vectors used.
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公开(公告)号：EP2038419A1
公开(公告)日：2009-03-25
申请号：EP07786975.8
申请日：2007-07-02
发明人： PAGET, Eric , DUBALD, Manuel , BALLY, Julia
IPC分类号： C12N15/82
CPC分类号： C12N15/8214
摘要： The present invention relates to nucleic acid sequences and methods useful in targeting a recombinant protein encoded by a transgene integrated into the chloroplast genome to the thylakoid lumen of chloroplast, whereby said nucleic acid sequences encode bacterial signal peptides. The invention also relates to means and methods for expressing a disulfide-bond containing protein of interest in a transplastomic plant cell.
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