公开(公告)号：EP2356465A1

公开(公告)日：2011-08-17

申请号：EP09826666.1

申请日：2009-11-11

申请人： Beckman Coulter, Inc.

发明人： ZHANG, Shuliang ,  LU, Jiuliu ,  ZHENG, Min ,  GRACE, Eric, M. ,  LI, Jing ,  LAVERNIA, Maritza ,  BRITTON, Ted, W.

IPC分类号： G01N33/72

CPC分类号： G01N33/721 ,  G01N33/491 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/25 ,  Y10T436/25125

摘要： A method of correction of particle interference to hemoglobin measurement of a blood sample on a hematology analyzer is provided. The method includes mixing an aliquot of a blood sample with a lytic reagent to lyse red blood cells and forming a sample mixture; measuring absorbance of the sample mixture at a predetermined wavelength of a hemoglobin chromogen formed in the sample mixture, and obtaining an apparent hemoglobin concentration of the blood sample using obtained absorbance; measuring concentration and size of cellular particles remaining in the sample mixture; removing contribution of the cellular particles to the apparent hemoglobin concentration using the concentration and the size of the cellular particles to obtain a corrected hemoglobin concentration of the blood sample; and reporting the corrected hemoglobin concentration of the blood sample.

公开(公告)号：EP2350611A1

公开(公告)日：2011-08-03

申请号：EP09819687.6

申请日：2009-10-02

申请人： Beckman Coulter, Inc.

发明人： BRITTON, Ted, W. ,  LU, Jiuliu ,  ROSE, Jeffrey, L.

IPC分类号： G01N21/00

CPC分类号： G01N15/1429 ,  G01N15/1459 ,  G01N2015/1037 ,  G01N2015/1461

摘要： Methods and systems substantially eliminate data representative of coincident events from particle analyzer data. A fluid sample containing particles for analysis is prepared. Using an electrical or optical measurement device, signals are sensed. Each signal corresponds to events detected in a sub-sample of the fluid sample flowing through a measurement region in the particle analyzer. The existence of coincidence in the events is determined based on measuring a peak and first and second points of each of the signals. The first and second points have a signal value corresponding to a predetermined portion of the peak. Results data based upon the coincident events and non-coincident events is generated. The results data is then analyzed. In various examples, the method is applicable to a variety of particle types, and may be implemented on different types of particle analyzers including hematology analyzer and flow cytometers.

公开(公告)号：EP2350611B1

公开(公告)日：2019-02-13

申请号：EP09819687.6

申请日：2009-10-02

申请人： Beckman Coulter, Inc.

发明人： BRITTON, Ted, W. ,  LU, Jiuliu ,  ROSE, Jeffrey, L.

IPC分类号： G01N15/14

公开(公告)号：EP1412740B1

公开(公告)日：2015-07-08

申请号：EP02752578.1

申请日：2002-07-25

申请人： Beckman Coulter, Inc.

发明人： LI, YI ,  LI, Jing ,  BRITTON, Ted, W.

IPC分类号： G01N33/80 ,  G01N15/12 ,  G01N15/14 ,  G01N15/00 ,  G01N15/10

CPC分类号： G01N15/12 ,  G01N15/14 ,  G01N33/80 ,  G01N2015/0069 ,  G01N2015/0073 ,  G01N2015/008 ,  G01N2015/1062 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497

公开(公告)号：EP2356465B1

公开(公告)日：2014-10-01

申请号：EP09826666.1

申请日：2009-11-11

申请人： Beckman Coulter, Inc.

发明人： ZHANG, Shuliang ,  LU, Jiuliu ,  ZHENG, Min ,  GRACE, Eric, M. ,  LI, Jing ,  LAVERNIA, Maritza ,  BRITTON, Ted, W.

IPC分类号： G01N33/72 ,  G01N33/49

CPC分类号： G01N33/721 ,  G01N33/491 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/25 ,  Y10T436/25125

公开(公告)号：EP1412740A1

公开(公告)日：2004-04-28

申请号：EP02752578.1

申请日：2002-07-25

申请人： Beckman Coulter, Inc.

发明人： LI, YI ,  LI, Jing ,  BRITTON, Ted, W.

IPC分类号： G01N31/00

CPC分类号： G01N15/12 ,  G01N15/14 ,  G01N33/80 ,  G01N2015/0069 ,  G01N2015/0073 ,  G01N2015/008 ,  G01N2015/1062 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497

摘要： A method for differentiating and enumerating nucleated red blood cells in a blood sample is described. The method includes the steps of lysing red blood cells of a blood sample with a lytic reagent, measuring nucleated blood cells by DC impedance measurement in a non-focused flow aperture, differentiating nucleated red blood cells from other cell types, and reporting nucleated red blood cells in the blood sample. The method further includes subtracting nucleated red blood cell and other interference materials from the count of remaining blood cells, and reporting a corrected white blood cell count of the blood sample. Additionally, the method further includes measuring spectrophotometric absorbance of the sample mixture at a predetermined wavelength of a hemoglobin chromogen formed upon lysing the blood sample, and reporting hemoglobin concentration of the blood sample.