公开(公告)号：EP1969362B1

公开(公告)日：2020-07-22

申请号：EP06846337.1

申请日：2006-11-17

申请人： Beckman Coulter, Inc.

发明人： ZHANG, Shuliang ,  ZHENG, Min ,  LIN, Dongqing ,  HUO, Ziling

IPC分类号： G01N15/10 ,  G01N15/12 ,  G01N35/00 ,  G01N31/00

公开(公告)号：EP1917527B1

公开(公告)日：2018-10-03

申请号：EP06813527.6

申请日：2006-08-18

申请人： Beckman Coulter, Inc.

发明人： ZHENG, Min ,  LI, Jing ,  LI, Yi ,  SWITZER, Michael, J.

IPC分类号： G01N33/49

CPC分类号： G01N33/491 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/25 ,  Y10T436/25125

摘要： Methods of prevention and correction of white blood cell interferences to the measurements of mean cell volume (MCV), red blood cell concentration (RBC) and hematocrit (Hct) of a blood sample are disclosed. The interference to MCV can be prevented by identifying a valley between the red blood cell and white blood cell modes on the red blood cell distribution histogram, defining a red blood cell region using the valley and calculating MCV within the defined region. Alternatively, a curve fit of the white blood cell population on the red blood cell distribution histogram can be used to exclude the white blood cells. RBC can be corrected by subtracting the white blood cell concentration obtained from the analysis of a second aliquot sample, when the white blood cell concentration (WBC) exceeds a predetermined criterion. Hct of the blood sample can be calculated using the obtained MCV and RBC.

公开(公告)号：EP2356465B1

公开(公告)日：2014-10-01

申请号：EP09826666.1

申请日：2009-11-11

申请人： Beckman Coulter, Inc.

发明人： ZHANG, Shuliang ,  LU, Jiuliu ,  ZHENG, Min ,  GRACE, Eric, M. ,  LI, Jing ,  LAVERNIA, Maritza ,  BRITTON, Ted, W.

IPC分类号： G01N33/72 ,  G01N33/49

CPC分类号： G01N33/721 ,  G01N33/491 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/25 ,  Y10T436/25125

公开(公告)号：EP1412730B1

公开(公告)日：2013-11-13

申请号：EP02750306.9

申请日：2002-07-25

申请人： Beckman Coulter, Inc.

发明人： TAYLOR, Richard, Lee ,  ZHENG, Min

IPC分类号： G01N15/12

CPC分类号： G01N15/12 ,  G01N2015/025 ,  G01N2015/1062

公开(公告)号：EP1969362A2

公开(公告)日：2008-09-17

申请号：EP06846337.1

申请日：2006-11-17

申请人： Beckman Coulter, Inc.

发明人： ZHANG, Shuliang ,  ZHENG, Min ,  LIN, Dongqing ,  HUO, Ziling

IPC分类号： G01N31/00

摘要： Systems and methods consistent with embodiments of the present invention provide a method for the measurement and analysis of particle counts in flow cytometry and hematology instruments. In some methods for the measurement and analysis of particle counts, a corrected histogram of particle distributions is calculated and used to obtain an accurate count of particles and an accurate measurement of other particle parameters.

公开(公告)号：EP1417481A1

公开(公告)日：2004-05-12

申请号：EP02748164.7

申请日：2002-07-15

申请人： Beckman Coulter, Inc.

发明人： HUO, Ziling ,  PARK, Jaesang ,  YAO, Wei ,  ZHANG, Shuliang ,  ZHENG, Min

IPC分类号： G01N31/00

CPC分类号： G01N15/12 ,  G01N15/1456 ,  G01N21/47 ,  G01N33/491 ,  G01N2015/1037 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497

摘要： A method of analyzing nucleated red blood cells (NRBCs) in a blood sample and further enumerating NRBCs is disclosed. The method includes lysing a first aliquot and a second aliquot of a blood sample separately with a first lysing reagent system and a second lysing reagent system; measuring the first sample mixture in a flow cell by DC, RF, and light scatter measurements; measuring cell distributions and counting remaining blood cells in the second sample mixture by DC impedance measurements in a non-focused flow aperture; analyzing blood cell distribution patterns obtained from measuring the first sample mixture and from measuring the second sample mixture respectively; and further performing a combined analysis to differentiate NRBCs from other cell types and determine numbers of NRBCs in the blood sample.

公开(公告)号：EP1417481B1

公开(公告)日：2017-12-20

申请号：EP02748164.7

申请日：2002-07-15

申请人： Beckman Coulter, Inc.

发明人： HUO, Ziling ,  PARK, Jaesang ,  YAO, Wei ,  ZHANG, Shuliang ,  ZHENG, Min

IPC分类号： G01N31/00 ,  G01N15/12 ,  G01N33/49 ,  G01N33/50

CPC分类号： G01N15/12 ,  G01N15/1456 ,  G01N21/47 ,  G01N33/491 ,  G01N2015/1037 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497

摘要： A method of analyzing nucleated red blood cells (NRBCs) in a blood sample and further enumerating NRBCs is disclosed. The method includes lysing a first aliquot and a second aliquot of a blood sample separately with a first lysing reagent system and a second lysing reagent system; measuring the first sample mixture in a flow cell by DC, RF, and light scatter measurements; measuring cell distributions and counting remaining blood cells in the second sample mixture by DC impedance measurements in a non-focused flow aperture; analyzing blood cell distribution patterns obtained from measuring the first sample mixture and from measuring the second sample mixture respectively; and further performing a combined analysis to differentiate NRBCs from other cell types and determine numbers of NRBCs in the blood sample.

公开(公告)号：EP2356465A1

公开(公告)日：2011-08-17

申请号：EP09826666.1

申请日：2009-11-11

申请人： Beckman Coulter, Inc.

发明人： ZHANG, Shuliang ,  LU, Jiuliu ,  ZHENG, Min ,  GRACE, Eric, M. ,  LI, Jing ,  LAVERNIA, Maritza ,  BRITTON, Ted, W.

IPC分类号： G01N33/72

CPC分类号： G01N33/721 ,  G01N33/491 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/25 ,  Y10T436/25125

摘要： A method of correction of particle interference to hemoglobin measurement of a blood sample on a hematology analyzer is provided. The method includes mixing an aliquot of a blood sample with a lytic reagent to lyse red blood cells and forming a sample mixture; measuring absorbance of the sample mixture at a predetermined wavelength of a hemoglobin chromogen formed in the sample mixture, and obtaining an apparent hemoglobin concentration of the blood sample using obtained absorbance; measuring concentration and size of cellular particles remaining in the sample mixture; removing contribution of the cellular particles to the apparent hemoglobin concentration using the concentration and the size of the cellular particles to obtain a corrected hemoglobin concentration of the blood sample; and reporting the corrected hemoglobin concentration of the blood sample.

公开(公告)号：EP1917527A2

公开(公告)日：2008-05-07

申请号：EP06813527.6

申请日：2006-08-18

申请人： Beckman Coulter, Inc.

发明人： ZHENG, Min ,  LI, Jing ,  LI, Yi ,  SWITZER, Michael, J.

IPC分类号： G01N33/48

CPC分类号： G01N33/491 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/25 ,  Y10T436/25125

摘要： Methods of prevention and correction of white blood cell interferences to the measurements of mean cell volume (MCV), red blood cell concentration (RBC) and hematocrit (Hct) of a blood sample are disclosed. The interference to MCV can be prevented by identifying a valley between the red blood cell and white blood cell modes on the red blood cell distribution histogram, defining a red blood cell region using the valley and calculating MCV within the defined region. Alternatively, a curve fit of the white blood cell population on the red blood cell distribution histogram can be used to exclude the white blood cells. RBC can be corrected by subtracting the white blood cell concentration obtained from the analysis of a second aliquot sample, when the white blood cell concentration (WBC) exceeds a predetermined criterion. Hct of the blood sample can be calculated using the obtained MCV and RBC.