公开(公告)号：EP1403633B1

公开(公告)日：2012-07-04

申请号：EP03020445.7

申请日：2003-09-12

申请人： Becton Dickinson and Company

发明人： Norton, Pierce ,  Javadi, Shervin ,  Vrane, David R.

IPC分类号： G01N15/14

CPC分类号： G01N15/14 ,  G01N15/1404 ,  Y10T436/101666 ,  Y10T436/117497 ,  Y10T436/119163

公开(公告)号：EP1388366A3

公开(公告)日：2004-05-12

申请号：EP03016773.8

申请日：2003-07-23

申请人： Becton, Dickinson and Company

发明人： Vrane, David R.

IPC分类号： B01F9/00

CPC分类号： B01F9/0014 ,  B01F15/00435 ,  B01F2015/00629 ,  B01F2015/00636 ,  B01F2215/0037

摘要： A system and method for maintaining a suspension of particles within a fluid are disclosed. A container (18) holding a sample having particles and fluid is rotated about its axis at a first calculated rate for a first calculated time interval and then rotated about its axis at a second calculated rate for a second calculated time interval such that the sample flow effects that result maintain a suspension of particles within the fluid and agitate the sample. A cycle of rotation, including the first and second flow rates, may be repeated for a desired number of times for continuous maintenance of the particle suspension. A controller (14) is programmed to rotate a motor (16) connected to a container holder (30) that rotatably drives the container about its axis. Sample (20) may be aspirated from the container and transported to an analytical device (12) while the container is being rotated. Because the rates of rotation of the cycle are relatively slow and because solid body rotation is not achieved during the cycle, the level of agitation that occurs results in only a negligible impact on the analytical device.

公开(公告)号：EP1391611B1

公开(公告)日：2006-10-18

申请号：EP03016774.6

申请日：2003-07-23

申请人： Becton Dickinson and Company

发明人： Vrane, David R. ,  Pierce O. Norton

IPC分类号： F04B49/06 ,  F04B11/00 ,  G01N1/00 ,  G01N15/14

CPC分类号： G05D16/2066 ,  F04B11/0025 ,  G01N15/1404 ,  G01N35/1095 ,  G01N2015/1409 ,  Y10T137/3118 ,  Y10T137/3124

公开(公告)号：EP1388366A2

公开(公告)日：2004-02-11

申请号：EP03016773.8

申请日：2003-07-23

申请人： Becton, Dickinson and Company

发明人： Vrane, David R.

IPC分类号： B01F9/00

CPC分类号： B01F9/0014 ,  B01F15/00435 ,  B01F2015/00629 ,  B01F2015/00636 ,  B01F2215/0037

摘要： A system and method for maintaining a suspension of particles within a fluid are disclosed. A container (18) holding a sample having particles and fluid is rotated about its axis at a first calculated rate for a first calculated time interval and then rotated about its axis at a second calculated rate for a second calculated time interval such that the sample flow effects that result maintain a suspension of particles within the fluid and agitate the sample. A cycle of rotation, including the first and second flow rates, may be repeated for a desired number of times for continuous maintenance of the particle suspension. A controller (14) is programmed to rotate a motor (16) connected to a container holder (30) that rotatably drives the container about its axis. Sample (20) may be aspirated from the container and transported to an analytical device (12) while the container is being rotated. Because the rates of rotation of the cycle are relatively slow and because solid body rotation is not achieved during the cycle, the level of agitation that occurs results in only a negligible impact on the analytical device.

摘要翻译： 一种用于在流体中保持颗粒悬浮液的系统和方法，光盘游离缺失。 保持具有颗粒和流体的样品的容器（18）在一个第一计算出的比率在第二计算的速率绕其轴线转动用于在第一计算出的时间间隔，然后围绕其轴线旋转用于检查的第二计算的时间间隔没有样品流 效果并维持结果颗粒的悬浮液内的流体和搅动样品。 旋转的一个周期，包括第一和第二流速，可以重复用于连续维持粒子悬浮液的所希望的次数。 控制器（14）被编程为旋转地连接到一个容器保持器（30）的马达（16）也可旋转地驱动绕其轴线的容器。 样品（20）可以从所述容器吸出，并在分析装置（12），而容器被旋转运来。 由于循环的旋转速率相对缓慢，因为固体旋转没有达到循环期间，搅动水平做了发生在只分析装置上的可忽略的影响的结果。

公开(公告)号：EP1403633A3

公开(公告)日：2004-04-07

申请号：EP03020445.7

申请日：2003-09-12

申请人： Becton Dickinson and Company

发明人： Norton, Pierce ,  Javadi, Shervin ,  Vrane, David R.

IPC分类号： G01N15/14

CPC分类号： G01N15/14 ,  G01N15/1404 ,  Y10T436/101666 ,  Y10T436/117497 ,  Y10T436/119163

摘要： A flow cell and flow cytometer in which a nozzle at the end of a flow channel is disposed on a removable substrate held at a registered location on a flow cell. Other elements including illumination optics, light collection optics, and the flow cell may then be positioned at fixed locations and would not require subsequent periodic adjustment. The registered location for positioning the nozzle allows removal and replacement of the nozzle key with the nozzle subsequently positioned in the identical location.

摘要翻译： 一种流动池和流式细胞仪，其中在流动通道的末端的喷嘴设置在保持在流动池上的配准位置处的可移除基底上。 包括照明光学器件，光收集光学器件和流动池的其他元件然后可以定位在固定位置并且不需要随后的周期性调整。 用于定位喷嘴的登记位置允许随后喷嘴位于相同位置的喷嘴钥匙的移除和更换。

公开(公告)号：EP1391611A2

公开(公告)日：2004-02-25

申请号：EP03016774.6

申请日：2003-07-23

申请人： Becton, Dickinson and Company

发明人： Vrane, David R. ,  Pierce O. Norton

IPC分类号： F04B11/00 ,  F04B23/02

CPC分类号： G05D16/2066 ,  F04B11/0025 ,  G01N15/1404 ,  G01N35/1095 ,  G01N2015/1409 ,  Y10T137/3118 ,  Y10T137/3124

摘要： A device for conditioning of fluid to minimize pulses from a pump source. A pump drives fluid from a vented fluid reservoir through a system including a combination of an attenuation tank and one or more orifice. Fluid is pumped into a plenum chamber that supplies fluid in a pulse free flow stream. A pressurized head over the fluid in the plenum chamber allows fluid delivery at a selected pressure. A volume sensor monitors the fluid level within the plenum tank and activates the pump if fluid level falls below a specified level.

摘要翻译： 用于调节流体以最小化来自泵浦源的脉冲的装置。 泵通过包括衰减箱和一个或多个孔的组合的系统驱动来自排放的流体储存器的流体。 将流体泵送到以无脉冲流动流提供流体的增压室。 在增压室中的流体上的加压头允许在选定压力下的流体输送。 体积传感器监测增压室内的液位，如果液位低于指定水平，则启动泵。

公开(公告)号：EP1391611A3

公开(公告)日：2005-04-06

申请号：EP03016774.6

申请日：2003-07-23

申请人： Becton Dickinson and Company

发明人： Vrane, David R. ,  Pierce O. Norton

IPC分类号： F04B49/06 ,  F04B11/00 ,  G01N1/00 ,  G01N15/14

CPC分类号： G05D16/2066 ,  F04B11/0025 ,  G01N15/1404 ,  G01N35/1095 ,  G01N2015/1409 ,  Y10T137/3118 ,  Y10T137/3124

摘要： A device for conditioning of fluid to minimize pulses from a pump source. A pump drives fluid from a vented fluid reservoir through a system including a combination of an attenuation tank and one or more orifice. Fluid is pumped into a plenum chamber that supplies fluid in a pulse free flow stream. A pressurized head over the fluid in the plenum chamber allows fluid delivery at a selected pressure. A volume sensor monitors the fluid level within the plenum tank and activates the pump if fluid level falls below a specified level.

公开(公告)号：EP1403633A2

公开(公告)日：2004-03-31

申请号：EP03020445.7

申请日：2003-09-12

申请人： Becton Dickinson and Company

发明人： Norton, Pierce ,  Javadi, Shervin ,  Vrane, David R.

IPC分类号： G01N15/14

CPC分类号： G01N15/14 ,  G01N15/1404 ,  Y10T436/101666 ,  Y10T436/117497 ,  Y10T436/119163

摘要： A flow cell and flow cytometer in which a nozzle at the end of a flow channel is disposed on a removable substrate held at a registered location on a flow cell. Other elements including illumination optics, light collection optics, and the flow cell may then be positioned at fixed locations and would not require subsequent periodic adjustment. The registered location for positioning the nozzle allows removal and replacement of the nozzle key with the nozzle subsequently positioned in the identical location.

摘要翻译： 与流式细胞仪一起使用的流动池包括流动池体; 样品输送管; 至少一个鞘流口; 比色皿加入流通池体; 渠道; 并且在通道的终点处的可移除喷嘴保持在流动池上的注册位置，在限定的三维位置处的注册位置和以注册的旋转方向。 与流式细胞仪一起使用的流动池包括流动池体（230）; 样品输送管（202）延伸到流动池体内; 流动池主体上的至少一个护套流动口，并且允许护套流动液体流通过流动池本体; 连接到流动池主体的比色皿（210） 通道（212）延伸穿过比色杯，其中来自样品输送管和鞘流口的液体流入通道; 以及在通道的末端处的可移除喷嘴（216），其保持在流动池上的注册位置，在限定的三维位置处的登记位置和以注册的旋转方向。