公开(公告)号：EP0753066A1

公开(公告)日：1997-01-15

申请号：EP94925680.0

申请日：1994-07-22

申请人： Beth Israel Hospital Association ,  WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH

发明人： O'DONNELL, Michael, A. ,  DUDA, Rosemary, B. ,  DeWOLF, William, C. ,  ALDOVINI, Anna ,  YOUNG, Richard, A. ,  MURRAY, Peter, J.

IPC分类号： A61K39 ,  C07K14 ,  C12N1 ,  C12N15 ,  A61K38

CPC分类号： A61K39/04 ,  A61K38/00 ,  A61K39/00 ,  C07K14/005 ,  C07K14/35 ,  C07K14/52 ,  C07K14/55 ,  C12N15/74 ,  C12N15/87 ,  C12N2740/16022 ,  C12N2740/16122 ,  C12N2740/16222 ,  C12R1/32 ,  Y10S435/863 ,  Y10S435/864 ,  Y10S435/865 ,  Y10S435/866

摘要： The present invention relates to genetically recombinant (genetically engineered) mycobacteria which express DNA of interest which has been incorporated into the mycobacteria and is expressed extrachromosomally (episomally or autonomously) in the recombinant mycobacteria under the control of a promoter. It particularly relates to recombinant M. bovis-BCG in which DNA of interest is expressed extrachromosomally under the control of a mycobacterial hsp promoter, such as hsp70 and hsp60. DNA of interest is defined herein as heterologous DNA (i.e., DNA from a source other than the mycobacterium into which it is introduced) and is all or a portion of a gene or genes encoding enzymes, cytokines, lymphokines and immunopotentiators.