公开(公告)号：EP0625581B1

公开(公告)日：1998-12-30

申请号：EP94107040.1

申请日：1994-05-05

申请人： BIOTEST AG

发明人： Horn, Jürgen, Dr.

IPC分类号： C12Q1/04 ,  C12Q1/18

CPC分类号： C12Q1/04 ,  C12Q2304/22 ,  G01N2333/35 ,  Y10S435/863 ,  Y10S435/864 ,  Y10S435/865 ,  Y10S435/866

公开(公告)号：EP0756633A1

公开(公告)日：1997-02-05

申请号：EP95915003.0

申请日：1995-04-03

申请人： CORNING CLINICAL LABORATORIES, INC.

发明人： THORNTON, Charles, G.

IPC分类号： C12Q1 ,  G01N33

CPC分类号： C12Q1/689 ,  C12Q1/6806 ,  G01N33/5695 ,  Y10S435/863 ,  Y10S435/864 ,  Y10S435/865 ,  Y10S435/866 ,  Y10S435/975 ,  Y10S514/852 ,  Y10S514/863 ,  Y10S514/864

摘要： A method for the preparation of Mycobacteria from any liquid, semisolid or exotic source is described. The extracted Mycobacterial sample is suitable for detection by culture and amplification.

公开(公告)号：EP0994944A1

公开(公告)日：2000-04-26

申请号：EP98933285.3

申请日：1998-07-09

申请人： THE GOVERNMENT OF THE UNITED STATES OF AMERICA as represented by the SECRETARY OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES

发明人： BARRY, Clifton, E., III ,  YUAN, Ying ,  CRANE, Deborah

IPC分类号： C12N15/01 ,  C12N1/36 ,  A61K39/04 ,  C07K14/35

CPC分类号： C12R1/32 ,  A61K39/04 ,  A61K2039/521 ,  C07K14/35 ,  Y10S435/863 ,  Y10S435/864 ,  Y10S435/865 ,  Y10S435/866

摘要： This invention provides for novel attenuated strains of Mycobacterium tuberculosis and M. bovis. Attenuation is achieved by eliminating or by downregulating the expression of the α-crystallin heat shock protein gene ('acr gene'). The invention provides for vaccines and methods of vaccinating mammals for protection against Mycobacterium sp. that cause tuberculosis.

公开(公告)号：EP0625581A1

公开(公告)日：1994-11-23

申请号：EP94107040.1

申请日：1994-05-05

申请人： BIOTEST AG

发明人： Horn, Jürgen, Dr.

IPC分类号： C12Q1/04 ,  C12Q1/18

CPC分类号： C12Q1/04 ,  C12Q2304/22 ,  G01N2333/35 ,  Y10S435/863 ,  Y10S435/864 ,  Y10S435/865 ,  Y10S435/866

摘要： Die Erfindung betrifft ein Verfahren zum optischen Nachweis des Wachstums von Mikroorganismen wie Pilzen, Hefen und Bakterien, insbesondere Mykobakterien, sowie zur Identifizierung und Empfindlichkeitstestung gegen Antibiotika durch visuelle Ermittlung des Farbumschlags eines spezifischen Redoxindikatorsystems, umfassend Methylenblau und Resazurin bzw. FeIII- oder FeII-Salze in Kombination mit K₃Fe(CN)₆ bzw. K₄Fe(CN)₆ oder Natriumwolframat (Na₂WO₄).

摘要翻译： 本发明涉及一种方法，用于光学地检测微生物如真菌，酵母和细菌，特别是分枝杆菌，以及用于通过视觉确定特定Redoxindikatorsystems的颜色变化的鉴定和药敏试验抗生素的生长，其包括亚甲基蓝和刃天青或FeIII或FeII盐 与K 3的Fe（CN）6和亚铁氰化钾6或钨酸钠（钨酸钠）组合。

公开(公告)号：EP0040407A1

公开(公告)日：1981-11-25

申请号：EP81103717.5

申请日：1981-05-14

申请人： Eisai Co., Ltd. ,  Ishizaka, Seiichi President of AGENCY OF INDUSTRIAL SCIENCE and TECHNOLOGY

发明人： Sato, Akio ,  Nakajima, Kenji ,  Takahara, Yoshimasa ,  Kijima, Shizumasa ,  Inai, Yuichi ,  Kohara, Yoshiyuki ,  Kawakami, Yoshiyuki ,  Tsurugi, Tomio

IPC分类号： C07C59/42 ,  C07C59/74 ,  C07C59/13 ,  C07C69/732 ,  C07C69/73 ,  C07C69/602 ,  C12P7/42 ,  C12P7/44 ,  C12P7/62 ,  C12P7/64 ,  A61K31/20

CPC分类号： C12P7/6409 ,  C07C57/13 ,  C07C59/42 ,  C07C59/74 ,  C12P7/42 ,  C12P7/44 ,  C12P7/62 ,  C12P7/6436 ,  C12R1/15 ,  C12R1/34 ,  C12R1/365 ,  C12R1/73 ,  Y02P20/55 ,  Y10S435/843 ,  Y10S435/866 ,  Y10S435/872 ,  Y10S435/923

摘要： A polyprenyl carboxylic acid derivative having the general formula (I):
in which n is an integer of from 2 to 4, R 1 represents the hydrogen atom or a protecting group for the carboxylic acid group, and R 2 represents a hydroxymethyl, formyl or carboxyl group. A process for the preparation of the polyprenyl carboxylic acid derivative involving microbiological oxidation using a strain belonging to the genus Nocardia is disclosed. The polyprenyl carboxylic acid derivative also exhibits pharmacological activity and is useful in form of a pharmaceutical composition.

摘要翻译： 具有通式（I）的聚戊烯羧酸衍生物：... ...其中n为2〜4的整数，R1表示氢原子或羧酸基的保护基，R2表示羟甲基 ，甲酰基或羧基。 公开了使用属于诺卡氏菌属的菌株制备涉及微生物氧化的聚异戊二烯羧酸衍生物的方法。 聚异戊二烯羧酸衍生物也表现出药理活性，可用于药物组合物的形式。

公开(公告)号：EP0876504B1

公开(公告)日：2002-10-30

申请号：EP96942457.1

申请日：1996-12-16

申请人： Biotec Laboratories Limited

发明人： WILSON, Stuart, Mark

IPC分类号： C12Q1/04 ,  C12Q1/06 ,  C12Q1/10 ,  C12N15/03

CPC分类号： C12Q1/06 ,  C12Q1/04 ,  C12Q1/10 ,  G01N2333/35 ,  Y10S435/864 ,  Y10S435/866

摘要： The present invention relates to a method for enhancing the time of response of an assay for a first bacterium, characterised in that: a) the said first bacterium is exposed to infection by phage particles to which the said first bacterium is permissive; b) the infected bacterium is treated to in-activate exogenous phage particles; c) the treated bacterium is cultivated in the presence of a second bacterium which is permissive to said phage or its replicand and which has a doubling rate greater than the effective doubling rate of the first bacterium; and d) assessing the extent of plaque formation and/or of second bacterium growth in the cultivated second bacterium cells. The method of the invention can be used to assess the presence of first bacterium in a sample, notably where the first bacterium is a slow growing bacterium, especially Mycobacterium tuberculosis, where the method of the invention enables an operator to detect the presence of low amounts of the bacterium in a sample within days instead of the weeks required with conventional bacterial cultivation techniques. The invention can also be used to assess the effect of a drug or other treatment on a bacterium or of a virus. The invention also provides a diagnostic kit for use in the method of the invention.

公开(公告)号：EP1012158A1

公开(公告)日：2000-06-28

申请号：EP97954594.4

申请日：1997-12-16

申请人： Southern Research Institute

发明人： BARROW, William, W. ,  VAN GINKEL, Sabrina, Z. ,  DOOLEY, Thomas, P. ,  SULING, William, J.

IPC分类号： C07H21/04 ,  C07H21/00 ,  C07K1/00 ,  C12N1/19 ,  C12N9/00 ,  C12N9/02 ,  C12N15/00 ,  C12P21/00 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/68

CPC分类号： C12N9/003 ,  C07K14/35 ,  C12Q1/04 ,  C12Q1/689 ,  Y10S435/863 ,  Y10S435/864 ,  Y10S435/865 ,  Y10S435/866

摘要： The invention relates to the nucleic acid sequence and amino acid sequence of dihydrofolate reductase (DHFR) from mycobacteria and to expression of recombinant DHFR protein. Utilizing the recombinant protein, novel therapies and diagnostic strategies can be developed and selective antimycobacterial compositions can be designed and utilized to treat mycobacterial infections in patients. This invention includes all or portions of novel recombinant nucleic acids encoding DHFR for mycobacteria such as M. avium, to novel recombinant DHFR peptides produced by such sequences, and to vaccines, diagnostic kits, cells and therapies utilizing these peptides and nucleic acid sequences. The present invention relates to methods for using the sequences of the present invention to develop drugs specific to M. avium and other mycobacteria, to identify and sequence corresponding sequences in species other than M. avium, as well as diagnostic and treatment methods incorporating the disclosed sequences and peptides.

公开(公告)号：EP0934421A1

公开(公告)日：1999-08-11

申请号：EP97933924.0

申请日：1997-08-06

申请人： Laboratorio Medinfar-Produtos Farmaceuticos LDA.

发明人： FRAZAO MONIZ PEREIRA, José, Antonio ,  FREITAS VIEIRA, Alcino ,  RIBEIRO DOS SANTOS ANES, Elsa, Maria ,  DA COSTA GARCIA, Miguel, Angelo ,  DA SILVA ALVES, Paulo, Jorge

IPC分类号： C12N1 ,  C12N9 ,  C12N15 ,  A61K39

CPC分类号： C12N9/22 ,  A61K39/00 ,  C12N15/74 ,  C12N15/90 ,  C12N2310/111 ,  C12N2799/021 ,  Y10S435/866

摘要： The present invention discloses a DNA fragment that can direct the insertion of heterologous DNA into a specific site (tRNAAla gene) of Mycobacterium spp. genome. This DNA fragment includes the attachment site region (attP) and the integrase gene of the mycobacteriophage Ms6. Heterologous DNA linked to this DNA fragment can be carried into the mycobacterial genome through a site-specific integration mechanism. A new DNA integration process is disclosed that consists in providing the integrase gene on a suicide vector, that transiently produces the integrase required for the insertion of the gene or genes linked to the Ms6 attP region contained in a separated plasmid vector. This process allows the construction of highly stable recombinant mycobacteria which may be used as vaccines and/or therapeutic vehicles.

公开(公告)号：EP0184511A2

公开(公告)日：1986-06-11

申请号：EP85402376.9

申请日：1985-12-02

申请人： ANDA BIOLOGICALS

发明人： Maes, Roland F.

IPC分类号： G01N33/531 ,  G01N33/569 ,  C12P21/00

CPC分类号： C12R1/32 ,  G01N33/531 ,  G01N33/5695 ,  Y10S435/803 ,  Y10S435/82 ,  Y10S435/863 ,  Y10S435/864 ,  Y10S435/866 ,  Y10S436/811 ,  Y10S436/815 ,  Y10S530/806 ,  Y10S530/82 ,  Y10S530/825

摘要： L'invention concerne un procédé d'isolement de l'antigène A 60, un procédé de production de fragments immunologiquement actifs à partir de celui-ci; leur utilisation pour détecter dans un sérum d'un mammifère la présence d'anticorps contre l'antigène A 60 ainsi qu'un procédé de détection de tels anticorps.
Le procédé d'isolement est caractérisé en ce qu'il comprend une étape de destruction des parois cellulaires de la mycobactérie: on sépare les débris cellulaires, notamment par centrifugation, on récolte le surnageant contenant le cytoplasme; on réalise une chromatographie d'exclusion en faisant passer le surnageant contenant le cytoplasme sur une colonne de gel d'exclusion et on récolte le pic d'exclusion contenant l'antigène A 60 substantiellement pur.
A partir de l'antigène A 60 ainsi isolé, on peut produire des fragments immunologiquement actifs; utiliser cet antigène et/ou ses fragments immunologiquement actifs à la détection d'anticorps et réaliser ainsi un procédé de détection desdits anticorps.

公开(公告)号：EP0996450A2

公开(公告)日：2000-05-03

申请号：EP98932735.8

申请日：1998-06-15

申请人： Keystone Biomedical, Inc.

发明人： HORWITZ, Lawrence, D. ,  HORWITZ, Kathryn, B.

IPC分类号： A61K31/55

CPC分类号： A61K31/55 ,  Y10S435/863 ,  Y10S435/864 ,  Y10S435/865 ,  Y10S435/866

摘要： The invention is directed toward the use of desferri-Exochelins to destroy cancer cells or retard or eliminate the growth of those cancer cells.