公开(公告)号：EP2768843B1

公开(公告)日：2017-08-30

申请号：EP12841318.4

申请日：2012-10-19

申请人： Bio-Rad Laboratories, Inc.

发明人： LIAO, Jiali ,  OLECH, Lee ,  CHEN, Hong ,  HE, Xuemei ,  FROST, Russell

IPC分类号： C07K1/16 ,  C07K1/22 ,  C07K1/36 ,  B01D15/10 ,  B01D15/20

CPC分类号： B01J39/26 ,  C07K1/165 ,  C07K1/22 ,  C07K16/065

公开(公告)号：EP2822687B1

公开(公告)日：2019-11-27

申请号：EP13758660.8

申请日：2013-03-05

申请人： Bio-rad Laboratories, Inc.

发明人： LIAO, Jiali ,  FROST, Russell

IPC分类号： B01D15/32

公开(公告)号：EP2768843A1

公开(公告)日：2014-08-27

申请号：EP12841318.4

申请日：2012-10-19

申请人： Bio-Rad Laboratories, Inc.

发明人： LIAO, Jiali ,  OLECH, Lee ,  CHEN, Hong ,  HE, Xuemei ,  FROST, Russell

IPC分类号： C07K1/16 ,  C07K1/22 ,  C07K1/36 ,  B01D15/10 ,  B01D15/20

CPC分类号： B01J39/26 ,  C07K1/165 ,  C07K1/22 ,  C07K16/065

摘要： Proteins are purified by a mixed-mode chromatography system formed by attaching a ligand with cation exchange and hydrophobic functionalities to a large-pore support matrix, the only linkage between the ligand and the support matrix being a chain having a backbone of no more than three atoms between the hydrophobic group and the support matrix.

公开(公告)号：EP2986625B1

公开(公告)日：2020-03-11

申请号：EP15761242.5

申请日：2015-03-13

申请人： Bio-rad Laboratories, Inc. ,  Rensselaer Polytechnic Institute

发明人： CRAMER, Steven ,  WOO, James ,  CHEN, Hong ,  LIAO, Jiali ,  FROST, Russell

IPC分类号： B01J20/286 ,  B01J20/28 ,  B01J20/32 ,  B01J39/26 ,  B01J39/20 ,  C07K1/16

公开(公告)号：EP2986625A2

公开(公告)日：2016-02-24

申请号：EP15761242.5

申请日：2015-03-13

申请人： Bio-rad Laboratories, Inc. ,  Rensselaer Polytechnic Institute

发明人： CRAMER, Steven ,  WOO, James ,  CHEN, Hong ,  LIAO, Jiali ,  FROST, Russell

IPC分类号： C07K1/22

CPC分类号： B01J39/20 ,  B01J20/28004 ,  B01J20/28085 ,  B01J20/286 ,  B01J20/321 ,  B01J20/3253 ,  B01J20/3285 ,  B01J39/26 ,  B01J2220/82 ,  C07K1/165

摘要： Mixed mode ligands, as well their use for purifying proteins, are provided.

公开(公告)号：EP2822687A2

公开(公告)日：2015-01-14

申请号：EP13758660.8

申请日：2013-03-05

申请人： Bio-rad Laboratories, Inc.

发明人： LIAO, Jiali ,  FROST, Russell

IPC分类号： B01J41/20 ,  C07K1/16

摘要： Solid supports and ligands are provided for purification of biomolecules by mixed-mode anion exchange-hydrophobic chromatography. Compositions can have the formula Support-(X)—N(R1, R2)—R3-L-Ar, or a salt thereof, wherein: Support is a chromatographic solid support; X is a spacer or absent; R1 and R2 are each selected from hydrogen and an alkyl comprising 1-6 carbons; R3 is an alkyl comprising 1-6 carbons or a cyclo alkyl comprising 1-6 carbons; L is NR4, O, or S; wherein R4 is hydrogen or an alkyl comprising 1-6 carbons; and Ar is an aryl. Methods are also provided for using solid supports and ligands to purify biomolecules such as monomeric antibodies.

摘要翻译： 提供了阴离子交换 - 疏水混合模式配体及其使用方法。