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1.发明公开
公开(公告)号：EP1692298A2
公开(公告)日：2006-08-23
申请号：EP04817449.4
申请日：2004-10-26
IPC分类号： C12Q1/00
CPC分类号： C12Q1/6832 , B01J2219/00459 , B01J2219/00576 , B01J2219/00608 , B01J2219/00648 , B01J2219/00722 , C12Q1/6809 , C12Q1/6834 , C12Q1/6837 , C12Q2537/143 , C12Q2565/501 , C12Q2565/507 , C12Q2533/101 , C12Q2565/514 , C12Q2565/519
摘要： Disclosed are methods of multiplexed analysis of oligonucleotides in a sample, including: methods of probe and target “engineering”, as well as methods of assay signal analysis relating to the modulation of the probe-target affinity constant, K by a variety of factors including the elastic properties of target strands and layers of immobilized (“grafted”) probes; and assay methodologies relating to: the tuning of assay signal intensities including dynamic range compression and on-chip signal amplification; the combination of hybridization-mediated and elongation-mediated detection for the quantitative determination of abundance of messages displaying a high degree of sequence similarity, including, for example, the simultaneous determination of the relative expression levels, and identification of the specific class of, untranslated AU-rich subsequences located near the 3′ terminus of mRNA; and a new method of subtractive differential gene expression analysis which requires only a single color label.
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公开(公告)号：EP1488236A2
公开(公告)日：2004-12-22
申请号：EP02798611.6
申请日：2002-12-27
发明人： SEUL, Michael , CHAU, Chiu, Wo , HUANG, Hui , BANERJEE, Sukanta , HONG, Ye , YANG, Jiacheng
IPC分类号： G01N33/543 , G01N33/533 , C07H1/00 , C07H21/00 , C12Q1/68
CPC分类号： C12Q1/6837 , B01J19/0046 , B01J2219/00274 , B01J2219/00317 , B01J2219/00466 , B01J2219/00468 , B01J2219/005 , B01J2219/00545 , B01J2219/00648 , B01J2219/00662 , G01N33/54306 , C12Q2563/149
摘要： The present invention provides methods for quality control of biochip manufacture, preferably where the substrate is a semiconductor and the biochip carries bio-functionalized microbeads.
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公开(公告)号：EP1488236B1
公开(公告)日：2012-10-31
申请号：EP02798611.6
申请日：2002-12-27
发明人： SEUL, Michael , CHAU, Chiu, Wo , HUANG, Hui , BANERJEE, Sukanta , HONG, Ye , YANG, Jiacheng
IPC分类号： G01N33/543 , G01N33/533 , C07H1/00 , C07H21/00 , C12Q1/68
CPC分类号： C12Q1/6837 , B01J19/0046 , B01J2219/00274 , B01J2219/00317 , B01J2219/00466 , B01J2219/00468 , B01J2219/005 , B01J2219/00545 , B01J2219/00648 , B01J2219/00662 , G01N33/54306 , C12Q2563/149
摘要： The present invention provides methods for quality control of biochip manufacture, preferably where the substrate is a semiconductor and the biochip carries bio-functionalized microbeads.
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公开(公告)号：EP1694859A2
公开(公告)日：2006-08-30
申请号：EP04796412.7
申请日：2004-10-26
发明人： YANG, Jiacheng
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q2565/519 , C12Q2523/107
摘要： A method of fragmentation of double stranded DNA is disclosed for use in nucleic acid analysis, notably in the multiplexed analysis of polymorphisms and mutations. The method produces a multiplicity of labeled sense and anti-sense fragments which are not complementary, and thus do not significantly re-anneal under conditions suitable for hybridization analysis (or capture-mediated elongation analysis) of the polymorphisms and/or mutations. The fragments display a desired or predicted length distribution. Cleavage sites can be selected such that the fragments are short, yet long enough to allow discrimination among fragments in an assay, and as a matter of statistical probability, such that the majority of fragments contain at least one labeled nucleotide to facilitate detection.
摘要翻译： 公开了用于核酸分析的双链DNA片段化方法，特别是在多态性和突变的多重分析中。 该方法产生多个标记的有义和反义片段，它们不互补，因此在适合于多态性和/或突变的杂交分析（或捕获介导的延伸分析）的条件下不显着再退火。 该片段显示期望的或预测的长度分布。 可以选择切割位点，使得片段短而足够长以允许在测定中片段之间的区分，并且作为统计概率的问题，使得大多数片段含有至少一个标记的核苷酸以便于检测。
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公开(公告)号：EP1694859B1
公开(公告)日：2015-01-07
申请号：EP04796412.7
申请日：2004-10-26
发明人： YANG, Jiacheng
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q2565/519 , C12Q2523/107
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6.发明公开GENOTYPING OF MULTIPLE LOCI WITH PCR FOR DIFFERENT LOCI AMPLIFICATION AT DIFFERENT TEMPERATURES 审中-公开基因分型多个位点的手段，进行PCR扩增不同基因座在不同的温度
公开(公告)号：EP1756312A1
公开(公告)日：2007-02-28
申请号：EP05750553.9
申请日：2005-05-17
发明人： YANG, Jiacheng
CPC分类号： C12Q1/6881 , C07H21/04 , C12Q1/686 , C12Q2600/16 , C12Q2527/15 , C12Q2537/143 , C12Q2527/101
摘要： Disclosed is a method of performing simulltaneous PCR amplification of several designated different loci in a sample each including a different target subsequence, using a set of pairs of forward and reverse primers, wherein the pairs are complementary to target subsequences, where different primer pairs are in different reaction chambers and the sample is also present in the reaction chambers, and wherein different primer pairs have different sequences. Different reaction chambers are provided different annealing temperatures, preferably at the same time, such that the annealing temperatures selected enhance annealing conditions for the primer pairs and the target subsequences within the reaction chambers. The method allows PCR to proceed more quickly, which is important to increase throughput in a multiplexed assay, and can be particularly important for HLA-typing in a transplantation setting.
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