摘要:
The present invention relates to a method for detecting, for at least one microorganism included in a sample, at least one marker for resistance to a glycopeptide, comprising the detection, by mass spectrometry, of at least one peptide or one protein of said microorganism. Preferentially, the glycopeptide is vancomycin. Preferentially, said peptide is derived from a vanA, vanB, vanC, vanD, vanE, vanG, vanL, vanM or vanN protein.
摘要:
The invention relates to a method for detecting and/or identifying, in a biological sample, bacteria having a resistance to carbapenems by means of the production of carbapenemase. The method comprises the following steps consisting in: a) bringing the sample into contact with a reaction medium containing at least one antibiotic from the carbapenem class and cloxacillin; b) incubating the sample and reaction medium brought into contact in step (a), such as to allow the growth of bacteria; c) detecting the strains having an enzymatic resistance to carbapenems. Advantageously, the medium used in step (a) also contains phenylalanine-arginine-beta-naphthylamide (PAbetaN).
摘要:
L'invention porte sur un procédé de détection, par spectrométrie de masse, d'au moins un marqueur d'au moins un mécanisme de résistance à au moins un antimicrobien, résistance d'au moins un microorganisme compris dans un échantillon, caractérisé en ce que l'antimicrobien est une céphalosporine, et lesdits marqueurs de résistance sont des protéines ou des peptides. De façon préférentielle, lesdits protéines ou peptides sont des protéines dudit microorganisme.
摘要:
The invention relates to a method for detecting and/or identifying, in a biological sample, bacteria having a resistance to carbapenems. The method comprises the following steps consisting in: a) bringing the sample into contact with a reaction medium containing at least one chromogenic agent and faropenem and/or doripenem; b) incubating the sample and reaction medium brought into contact in step (a), such as to allow the growth of bacteria; and c) detecting the strains having a resistance to carbapenems. The medium used in step (a) also contains cloxacillin and/or a combination of cloxacillin and PAbetaN.