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1.发明授权METHODS FOR PRODUCING FUNCTIONAL, SINGLE-CHAIN Fv ANTIBODY FRAGMENTS ON THE SURFACE OF BACTERIOPHAGE PARTICLES 失效METHOD FOR产生功能性，单链Fv抗体片段噬菌体ON SURFACES
公开(公告)号：EP0589877B1
公开(公告)日：1996-11-13
申请号：EP91913039.3
申请日：1991-07-10
发明人： McCAFFERTY, John , POPE, Anthony, Richard , JOHNSON, Kevin Stuart , HOOGENBOOM, Hendricus Renerus Jacobus Mattheus , GRIFFITHS, Andrew David , JACKSON, Ronald, Henry , HOLLIGER, Kaspar, Philipp , MARKS, James David , CLACKSON, Timothy, Piers , CHISWELL, David John , WINTER, Gregory, Paul , BONNERT, Timothy, Peter
IPC分类号： C12N7/01 , C07K1/04 , G01N33/531
CPC分类号： C07K16/005 , B01J2219/00718 , C07K16/00 , C07K16/18 , C07K16/26 , C07K16/34 , C07K16/40 , C07K16/44 , C07K16/462 , C07K16/468 , C07K19/00 , C07K2317/21 , C07K2317/24 , C07K2317/34 , C07K2317/55 , C07K2317/62 , C07K2317/622 , C07K2317/92 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62 , C12N15/73 , C40B40/02 , G01N33/53 , G01N33/6857 , Y10S530/867
摘要： A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.
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2.发明授权METHODS FOR PRODUCING FUNCTIONAL, SINGLE-CHAIN Fv ANTIBODY FRAGMENTS ON THE SURFACE OF BACTERIOPHAGE PARTICLES 失效METHOD FOR产生功能性，单链Fv抗体片段噬菌体ON SURFACES
公开(公告)号：EP0589877B2
公开(公告)日：2005-10-26
申请号：EP91913039.3
申请日：1991-07-10
发明人： McCAFFERTY, John , POPE, Anthony, Richard , JOHNSON, Kevin Stuart , HOOGENBOOM, Hendricus Renerus Jacobus Mattheus , GRIFFITHS, Andrew David , JACKSON, Ronald, Henry , HOLLIGER, Kaspar, Philipp , MARKS, James David , CLACKSON, Timothy, Piers , CHISWELL, David John , WINTER, Gregory, Paul , BONNERT, Timothy, Peter
IPC分类号： C12N7/01 , C07K1/04 , G01N33/531
CPC分类号： C07K16/005 , B01J2219/00718 , C07K16/00 , C07K16/18 , C07K16/26 , C07K16/34 , C07K16/40 , C07K16/44 , C07K16/462 , C07K16/468 , C07K19/00 , C07K2317/21 , C07K2317/24 , C07K2317/34 , C07K2317/55 , C07K2317/62 , C07K2317/622 , C07K2317/92 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62 , C12N15/73 , C40B40/02 , G01N33/53 , G01N33/6857 , Y10S530/867
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公开(公告)号：EP0589877A1
公开(公告)日：1994-04-06
申请号：EP91913039.0
申请日：1991-07-10
发明人： McCAFFERTY, John , POPE, Anthony, Richard , JOHNSON, Kevin Stuart , HOOGENBOOM, Hendricus Renerus Jacobus Mattheus , GRIFFITHS, Andrew David , JACKSON, Ronald, Henry , HOLLIGER, Kaspar, Philipp , MARKS, James David , CLACKSON, Timothy, Piers , CHISWELL, David John , WINTER, Gregory, Paul , BONNERT, Timothy, Peter
CPC分类号： C07K16/005 , B01J2219/00718 , C07K16/00 , C07K16/18 , C07K16/26 , C07K16/34 , C07K16/40 , C07K16/44 , C07K16/462 , C07K16/468 , C07K19/00 , C07K2317/21 , C07K2317/24 , C07K2317/34 , C07K2317/55 , C07K2317/62 , C07K2317/622 , C07K2317/92 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62 , C12N15/73 , C40B40/02 , G01N33/53 , G01N33/6857 , Y10S530/867
摘要： On identifie un membre d'une paire à liaison spécifique (sbp) en exprimant l'ADN codant une colonie à diversité génétique de ces membres de sbp dans des cellules hôtes recombinées dans lesquelles les membres de sbp se présentent sous forme fonctionnelle à la surface d'un capside de présentation génétique de recombinaison secrété (rgdp) renfermant l'ADN codant le membre sbp ou un constituant polypeptidique de celui-ci, grâce à l'expression de ce membre de sbp ou de son constituant polypeptidique sous forme d'une fusion avec un constituant d'encapsidation du rgdp. On peut sélectionner les sbp présentées selon l'affinité avec un membre de sbp complémentaire, et récupérer l'ADN des rgdp sélectionnés pour l'expression des membres de sbp sélectionnés. On peut ainsi obtenir des membres anticorporaux de sbp dont les diverses chaînes sont exprimées, l'une étant fusionnée au constituant d'encapsidation et l'autre étant sous forme libre permettant une association au polypeptide partenaire de fusion. Un phagemide peut servir de vecteur d'expression et la fusion d'encapsidation contribue à l'encapsidation de l'ADN du phagemide. A l'aide de ce procédé, on peut associer des banques d'ADN codant des chaînes respectives de ces membres multimères de sbp, afin d'obtenir des membres de sbp ayant une plus grande diversité génétique que celle que l'on obtiendrait facilement à l'aide des procédés traditionnels.
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4.发明授权MULTIVALENT AND MULTISPECIFIC BINDING PROTEINS, THEIR MANUFACTURE AND USE 失效多VALENTE和多特异性结合蛋白，其生产和使用
公开(公告)号：EP0672142B1
公开(公告)日：2001-02-28
申请号：EP94902030.9
申请日：1993-12-03
发明人： HOLLIGER, Kaspar-Philipp , GRIFFITHS, Andrew David , HOOGENBOOM, Hendricus Renerus Jacobus Matheus , MALMQVIST, Magnus , MARKS, James David , McGUINNESS, Brian Timothy , POPE, Anthony Richard , PROSPERO, Terence Derek , WINTER, Gregory Paul
CPC分类号： C07K16/005 , A61K38/00 , C07K16/00 , C07K16/40 , C07K16/44 , C07K16/468 , C07K2317/34 , C07K2317/622 , C07K2317/626 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/113 , C12N2310/111 , C12N2310/1241 , C12N2310/127 , C40B40/02
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5.发明公开MULTIVALENT AND MULTISPECIFIC BINDING PROTEINS, THEIR MANUFACTURE AND USE 失效多VALENTE和多特异性结合蛋白，它们的生产和使用。
公开(公告)号：EP0672142A1
公开(公告)日：1995-09-20
申请号：EP94902030.0
申请日：1993-12-03
发明人： HOLLIGER, Kaspar-Philipp , GRIFFITHS, Andrew David , HOOGENBOOM, Hendricus Renerus Jacobus Matheus , MALMQVIST, Magnus , MARKS, James David , McGUINNESS, Brian Timothy , POPE, Anthony Richard , PROSPERO, Terence Derek , WINTER, Gregory Paul
IPC分类号： G01N33 , A61K39 , C07K1 , C07K2 , C07K16 , C07K19 , C12N1 , C12N15 , C12P21 , C40B40 , A61K38 , C12R1
CPC分类号： C07K16/005 , A61K38/00 , C07K16/00 , C07K16/40 , C07K16/44 , C07K16/468 , C07K2317/34 , C07K2317/622 , C07K2317/626 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/113 , C12N2310/111 , C12N2310/1241 , C12N2310/127 , C40B40/02
摘要： Polypeptides comprising a first domain, which comprises a binding region of an immunoglobulin heavy chain variable region, and a second domain, which comprises a binding region of an immunoglobulin light chain variable region, the domains being linked but incapable of associating with each other to form an antigen binding site, associate to form antigen binding multimers, such as dimers, which may be multivalent or have multispecificity. The domains may be linked by a short peptide linker or may be joined directly together. Bispecific dimers may have longer linkers. Methods of preparation of the polypeptides and multimers and diverse repertoires thereof, and their display on the surface of bacteriophage for easy selection of binders of interest, are disclosed, along with many utilities.
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