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21 条记录 (耗时 0.018 秒)

    Process for detecting target nucleic acid, process for quantifying the same, and pyrylium compound for chemiluminescence analysis
    5.
    发明公开
    Process for detecting target nucleic acid, process for quantifying the same, and pyrylium compound for chemiluminescence analysis 失效
    一种用于检测靶核酸，方法用于化学发光分析其量化和吡喃鎓化合物的方法

    公开(公告)号：EP0837141A2

    公开(公告)日：1998-04-22

    申请号：EP97307682.1

    申请日：1997-09-30

    申请人： CANON KABUSHIKI KAISHA

    发明人： Okamoto, Tadashi ,  Yamamoto, Nobuko

    IPC分类号： C12Q1/68 ,  C07D309/34 ,  C07D335/02

    CPC分类号： C07D335/02 ,  C07D309/34 ,  C12Q1/6813 ,  C12Q1/6816

    摘要： The present invention provides a process for detecting or quantifying a target nucleic acid in a sample, the process comprising the steps of associating a chemiluminescent compound, capable of being associated with a double-stranded nucleic acid, with a double-stranded nucleic acid including the target nucleic acid, and detecting or measuring chemiluminescence derived from the chemiluminescent compound associated with the double-stranded nucleic acid. According to the process, the target nucleic acid in the sample can be highly sensitively detected, or precisely quantified.

    摘要翻译： 本发明提供用于检测或定量样品中靶核酸的方法，该方法包括化学发光化合物缔合的步骤，能够与双链核酸相关，与双链核酸包括 靶核酸，并且检测或从与双链核酸相关联的化学发光化合物衍生的测量化学发光。 。根据该方法，所述目标核酸的样品中可以高度灵敏地检测，或精确地定量。

    Novel pyrylium compound, process for making the same, nucleic acid stain, and labeled nucleic acid
    7.
    发明公开
    Novel pyrylium compound, process for making the same, nucleic acid stain, and labeled nucleic acid 有权
    新吡喃鎓化合物，涉及它们的制备标记的核酸的过程，核酸染料和

    公开(公告)号：EP1070709A1

    公开(公告)日：2001-01-24

    申请号：EP00115763.5

    申请日：2000-07-21

    申请人： CANON KABUSHIKI KAISHA

    发明人： Suzuki, Tomohiro ,  Okamoto, Tadashi ,  Yamamoto, Nobuko

    IPC分类号： C07D309/34 ,  C12Q1/68 ,  G01N33/52 ,  C07D335/02

    CPC分类号： C07D335/02 ,  C07D309/34

    摘要： A novel pyrylium compound is represented by the general formula (I):
    wherein X is oxygen or sulfur, Y - is a monovalent anion, n is an integer of 2 or 3, and M is hydrogen or an alkali metal. This pyrylium compound functions as a labeling agent by intermolecular bonding to nucleic acids, and as a fluorescence label having a chemical bond with the nucleic acids and an excitation wavelength in a visible light region.

    摘要翻译： 一种新的吡喃鎓化合物由通式（I）表示： worin X是氧或硫，Y - 或氢是一价阴离子，n是2或3的整数，和M是碱金属<> ， 该吡喃鎓化合物用作由分子间的结合标记试剂的核酸，并且为具有化学键与所述核酸的荧光标签和在可见光区域的激发波长。