公开(公告)号：EP3004858B1

公开(公告)日：2018-07-04

申请号：EP14730740.9

申请日：2014-05-30

申请人： Boehringer Ingelheim Vetmedica GmbH

发明人： SCHIEBER, Markus ,  SCHOEDER, Heinz

IPC分类号： G01N27/403 ,  G01N27/30 ,  B01L3/00

CPC分类号： H01L22/32 ,  B01J2219/00317 ,  B01J2219/00619 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00653 ,  B01L3/5088 ,  B01L2200/12 ,  B01L2300/0645 ,  B01L2300/0819 ,  B01L2300/0861 ,  B01L2300/0887 ,  G01N27/403 ,  H01L29/401

公开(公告)号：EP3234234A4

公开(公告)日：2018-05-23

申请号：EP15869470

申请日：2015-12-15

申请人： NOVELLUSDX LTD

发明人： SHEINMAN YEHOSHUA ,  SHARIVKIN REVITAL ,  BELENKOVICH MERAV

IPC分类号： C40B60/08 ,  C12M1/18 ,  C12M3/00

CPC分类号： C12M33/06 ,  B01J2219/00351 ,  B01J2219/00369 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00734 ,  B01J2219/00743 ,  C12M1/18 ,  C12M3/00 ,  C12M33/14 ,  C40B60/08

摘要： The present disclosure provides systems, devices and methods for seeding cells or sets of molecules on a substrate by utilizing a seeding mesh, to obtain an essentially homogenous patterned seeding of the cells or sets of molecules on the mesh.

公开(公告)号：EP3017065A1

公开(公告)日：2016-05-11

申请号：EP14741745.5

申请日：2014-06-26

申请人： Illumina, Inc.

发明人： BERTI, Lorenzo ,  BROWN, Andrew A. ,  GEORGE, Wayne N.

IPC分类号： C12Q1/68 ,  B01J19/00 ,  C07H21/00 ,  C09D133/26

CPC分类号： C08J7/12 ,  B01J19/0046 ,  B01J2219/00529 ,  B01J2219/00533 ,  B01J2219/00619 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00722 ,  C08F8/00 ,  C08F2220/603 ,  C08J2335/00 ,  C09D133/26 ,  C12Q1/6837 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2527/125 ,  C12Q2565/537 ,  C12Q2565/629

摘要： Some embodiments described herein relate to a substrate with a surface comprising a silane or a silane derivative covalently attached to optionally substituted cycloalkene or optionally substituted heterocycloalkene for direct conjugation with a functionalized molecule of interest, such as a polymer, a hydrogel, an amino acid, a nucleoside, a nucleotide, a peptide, a polynucleotide, or a protein. In some embodiments, the silane or silane derivative contains optionally substituted norbornene or norbornene derivatives. Method for preparing a functionalized surface and the use in DNA sequencing and other diagnostic applications are also disclosed.

公开(公告)号：EP2934752A1

公开(公告)日：2015-10-28

申请号：EP13811380.8

申请日：2013-12-07

申请人： Karlsruher Institut für Technologie

发明人： RAPP, Bastian

IPC分类号： B01L3/02 ,  B41J2/015

CPC分类号： B05D1/28 ,  B01J2219/00378 ,  B01J2219/00382 ,  B01J2219/00385 ,  B01J2219/00619 ,  B01J2219/00711 ,  B01L3/0268 ,  B01L2300/0819 ,  B01L2300/165 ,  B01L2400/0406 ,  B01L2400/0427 ,  B01L2400/0454 ,  B05C1/00 ,  H01L51/0004

摘要： The invention relates to a method for transferring a transfer fluid from a supply surface (301) into a plurality of discrete compartments (102, 102', 102''...) on a target surface (101). The invention also relates to a transfer surface (201), which has a plurality of discrete compartments (202, 202', 202''...), wherein each compartment can be switched over between a first wetting behaviour, with respect to the transfer fluid, and a second wetting behaviour that is different from the first. Initially, all of the compartments (203, 203', 203''...) on the transfer surface (201) are set to the first wetting behaviour, before selected compartments (205, 205', 205''...) are subsequently configured for the second wetting behaviour. Then, the transfer surface (201) is brought into contact with the supply surface (301), whereby the selected compartments (205, 205', 205''...) receive transfer fluid (302) from the supply surface (301). After the transfer surface (201) has been removed from the supply surface (301), the transfer surface (201) is positioned above the target surface (101). After clearing the configuration of the selected compartments (303, 303', 303''), the transfer fluid (302) leaves the selected compartments (303, 303', 303'') of the transfer surface (201) and is thus transferred to the compartments (102, 102', 102'') of the target surface (101).

公开(公告)号：EP1257805B1

公开(公告)日：2015-10-14

申请号：EP01914366.8

申请日：2001-02-12

申请人： Illumina, Inc.

发明人： Dickinson, Todd

IPC分类号： G01N21/00

CPC分类号： G01N21/6452 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/005 ,  B01J2219/00511 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00628 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00644 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00662 ,  B01J2219/00677 ,  B01J2219/00702 ,  B01J2219/0072 ,  B01L3/5085 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0822 ,  B82Y30/00 ,  C40B60/14 ,  G01N21/6428

公开(公告)号：EP2856177A1

公开(公告)日：2015-04-08

申请号：EP13794324.7

申请日：2013-03-15

申请人： The University Of North Carolina At Chapel Hill

发明人： RAMSEY, John Michael ,  HENLEY, William ,  OBLATH, Emily

IPC分类号： G01N35/08 ,  G01N33/53 ,  G01N27/26 ,  C12Q1/68

CPC分类号： C12Q1/6823 ,  B01J2219/00317 ,  B01J2219/00454 ,  B01J2219/00495 ,  B01J2219/005 ,  B01J2219/00554 ,  B01J2219/00576 ,  B01J2219/00619 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/0829 ,  B01L2300/0864 ,  C12Q1/6853 ,  C12Q1/686 ,  G01N2035/00158 ,  C12Q2563/149 ,  C12Q2565/629 ,  C12Q2537/143 ,  C12Q2565/537

摘要： A microfluidic device includes a plurality of reaction wells; and a plurality of solid supports, and each of the solid supports has a reagent attached thereto. The reagent is attached to the solid support via a labile reagent/support bond such that the reagent is configured to be cleaved from the support via a cleaving operation.

公开(公告)号：EP2685266A4

公开(公告)日：2014-01-15

申请号：EP12754743

申请日：2012-03-07

申请人： JAPAN SCIENCE & TECH AGENCY

发明人： NOJI HIROYUKI ,  IINO RYOTA ,  ARAKI SUGURU

IPC分类号： G01N35/02 ,  B01J19/00 ,  G01N21/64 ,  G01N33/53 ,  G01N37/00

CPC分类号： G01N33/54386 ,  B01J2219/00317 ,  B01J2219/00466 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01L3/502715 ,  B01L3/502761 ,  B01L3/50853 ,  B01L2200/0642 ,  B01L2200/0668 ,  B01L2200/12 ,  B01L2300/0627 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0858 ,  B01L2300/0877 ,  B01L2300/161 ,  B01L2300/165 ,  G01N21/6452 ,  G01N33/54306 ,  G01N33/54313

摘要： This invention provides a technique enabling to detect target molecules of low concentration with high sensitivity. This invention includes (i) a step of introducing a hydrophilic solvent (42) containing beads (40),(41') into a space (30) between (a) a lower layer section (10) including a plurality of receptacles (13) each of which is capable of storing only one of the beads (41),(41') and which are separated from each other by a side wall (12) having a hydrophobic upper surface and (b) an upper layer section (20) facing a surface of the lower layer section (10) on which surface the plurality of receptacles (13) are provided; and (ii) a step of introducing a hydrophobic solvent (43) into the space (30), the step (ii) being carried out after the step (i).

公开(公告)号：EP2576058A1

公开(公告)日：2013-04-10

申请号：EP11724618.1

申请日：2011-06-07

申请人： Commissariat à l'Énergie Atomique et aux Énergies Alternatives

发明人： VIGNOUD, Séverine ,  NONGLATON, Guillaume

IPC分类号： B01L3/00

CPC分类号： B05D1/32 ,  B01J2219/00605 ,  B01J2219/00619 ,  B01J2219/00644 ,  B32B33/00 ,  G01N33/50 ,  G03F7/00 ,  G03F7/40 ,  Y10T428/24802

摘要： The present invention relates to a method for producing a deposit of a material, which is localized and has a defined shape, on the surface of a substrate, including the steps which involve (1) defining, by photolithography, on the surface of said substrate, at least one site which is localized, of defined shape, and wettable by a solution, and which contains said material, or from which said material is obtained, the areas defining and particularly surrounding said site being non-wettable by said solution, and (2) depositing said solution on said site and on said areas, so that said material is deposited on said site.

公开(公告)号：EP2465943A3

公开(公告)日：2012-10-03

申请号：EP10075555.2

申请日：2002-03-18

申请人： Mir, Kalim

发明人： Mir, Kalim

IPC分类号： C12Q1/68 ,  B01J19/00 ,  B82Y10/00 ,  B82Y30/00

CPC分类号： C12N15/1065 ,  B01J2219/00497 ,  B01J2219/005 ,  B01J2219/00513 ,  B01J2219/0052 ,  B01J2219/00524 ,  B01J2219/00554 ,  B01J2219/00572 ,  B01J2219/00576 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/0072 ,  B82Y10/00 ,  B82Y30/00 ,  C12Q1/6818 ,  C12Q1/6837 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2600/156 ,  C12Q2600/158 ,  G01N33/582 ,  C12Q2523/303 ,  C12Q2565/507 ,  C12Q2565/629

摘要： The invention comprises methods for analysing biomolecules. In particular the methods comprise approaches for deciphering sequence and/or the long-range order of sequence using methods that analyse multiple point signals along individual long linear nucleic acid molecules. The invention also comprises arrays of long linear nucleic acid molecules and kits and instrumentation for preparing the sample molecules and a system for analysing the molecules and reconstructing/re-assembling the sequence.

公开(公告)号：EP2478372A1

公开(公告)日：2012-07-25

申请号：EP10754327.4

申请日：2010-09-15

申请人： UNIVERSITE DE ROUEN

发明人： RIPOLL, Camille ,  NORRIS, Victor ,  LEGENT, Guillaume ,  DELAUNE, Anthony

IPC分类号： G01N33/68 ,  G01N23/225

CPC分类号： G01N33/6848 ,  B01J2219/00527 ,  B01J2219/00619 ,  B01J2219/00637 ,  B01J2219/00702 ,  B01J2219/00725 ,  C12Q1/02 ,  C12Q1/6837 ,  C40B20/02 ,  C40B20/08 ,  G01N33/50 ,  G01N2458/15

摘要： The present invention relates to an improved method for detecting and quantifying the presence or absence of a number of biomolecules in a sample using the SIMS technique and arrays for use in said method.