公开(公告)号：EP0950184A1

公开(公告)日：1999-10-20

申请号：EP97931456.0

申请日：1997-06-26

申请人： CELLSTAT TECHNOLOGIES, INC.

发明人： MALIN, Patricia, J. ,  WADA, Kenneth, R. ,  DEHLINGER, Peter, J.

IPC分类号： B01J19 ,  B01L3 ,  G01N33 ,  C40B60

CPC分类号： G01N33/5008 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00617 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00637 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00702 ,  B01J2219/00713 ,  B01L3/5085 ,  C40B60/14 ,  G01N33/502

摘要： High-throughput screening method and apparatus are described. The method includes placing cells on a substrate defining a plurality of discrete microwells, at a well density of greater than about 100/cm2, with the number of cells in each well being less than about 1000, and where the cells in each well have been exposed to a selected agent. The change in conductance in each well is determined by applying a low-voltage, AC signal across a pair of electrodes placed in that well, and synchronously measuring the conductance across the electrodes, to monitor the level of growth or metabolic activity of cells contained in each well. Also disclosed is an apparatus for carrying out the screening method.