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公开(公告)号：EP1405919B1
公开(公告)日：2008-03-12
申请号：EP03025616.8
申请日：1992-07-29
CPC分类号： C12M23/12 , C12M35/02 , C12M41/46 , G01N33/48735
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2.发明授权AN IMPROVED SYSTEM FOR ELECTRONICALLY MONITORING AND RECORDING CELL CULTURES 失效一种改进的系统，电子监控和录像的细胞培养物
公开(公告)号：EP0655086B1
公开(公告)日：2003-12-17
申请号：EP92916640.3
申请日：1992-07-29
摘要： This invention provides an improved system for monitoring and recording cell cultures. An improved lid (10) for the system includes pin sockets (28) interposed between traces (24) on a printed circuit board (22) and elongated, electrically conductive pins (38). A method for measuring a delay period for an alternating current voltage applied across a pair of pins (38) improves the stability of measurements made with the improved system. A method for applying a voltage across a pair of pins (38) which avoids exceeding a pre-established voltage avoids the possibility of damaging and/or destroying cells while monitoring their culture. Subtracting electrical conductivity measured for a reference well (16) that holds only cell growth media without cells from the electrical conductivity measured for other wells (16) during analysis of recorded data improves the presentation of test results obtained by monitoring and recording cell cultures.
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3.发明公开A method for electronically monitoring and recording cell cultures 失效维尔法赫恩zur elektronischenÜberwachungund Aufzeichnung von Zellkulturen
公开(公告)号：EP1405919A1
公开(公告)日：2004-04-07
申请号：EP03025616.8
申请日：1992-07-29
CPC分类号： C12M23/12 , C12M35/02 , C12M41/46 , G01N33/48735
摘要： This invention provides an improved system for monitoring and recording cell cultures. An improved lid (10) for the system includes pin sockets (28) interposed between traces (24) on a printed circuit board (22) and elongated, electrically conductive pins (38). A method for measuring a delay period for an alternating current voltage applied across a pair of pins (38) improves the stability of measurements made with the improved system. A method for applying a voltage across a pair of pins (38) which avoids exceeding a pre-established voltage avoids the possibility of damaging and/or destroying cells while monitoring their culture. Subtracting electrical conductivity measured for a reference well (16) that holds only cell growth media without cells from the electrical conductivity measured for other wells (16) during analysis of recorded data improves the presentation of test results obtained by monitoring and recording cell cultures.
摘要翻译： 电子监测在多细胞盘中培养的细胞。 appts。 包括一个盖子，其在使用中阻塞井口的开口并且在其顶表面上包括一个PCB。 一系列针插座，一个用于PCB的每个迹线，通过盖子，每个支撑一个导电销，定位成进入一个井。 一对引脚进入每个阱，使得可以跨越它们施加电压，并将所得到的信号传送到电路以使用计算机进行监视。
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公开(公告)号：EP0950184A1
公开(公告)日：1999-10-20
申请号：EP97931456.0
申请日：1997-06-26
CPC分类号： G01N33/5008 , B01J19/0046 , B01J2219/00317 , B01J2219/00605 , B01J2219/0061 , B01J2219/00617 , B01J2219/00619 , B01J2219/00621 , B01J2219/00637 , B01J2219/00653 , B01J2219/00659 , B01J2219/00702 , B01J2219/00713 , B01L3/5085 , C40B60/14 , G01N33/502
摘要： High-throughput screening method and apparatus are described. The method includes placing cells on a substrate defining a plurality of discrete microwells, at a well density of greater than about 100/cm2, with the number of cells in each well being less than about 1000, and where the cells in each well have been exposed to a selected agent. The change in conductance in each well is determined by applying a low-voltage, AC signal across a pair of electrodes placed in that well, and synchronously measuring the conductance across the electrodes, to monitor the level of growth or metabolic activity of cells contained in each well. Also disclosed is an apparatus for carrying out the screening method.
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公开(公告)号：EP1861489A1
公开(公告)日：2007-12-05
申请号：EP06739306.6
申请日：2006-03-22
发明人： MALIN, Patricia J.
IPC分类号： C12M1/34 , G01N33/487
摘要： The present invention provides a method of profiling cells. In the method, cells from a population of cells are distributed in a culture well provided with electrodes and with the requisite medium and cultured. Conductance is detected at various time intervals. The measured values of conductance are compared with reference data.
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6.发明公开
公开(公告)号：EP0655086A1
公开(公告)日：1995-05-31
申请号：EP92916640.0
申请日：1992-07-29
摘要： This invention provides an improved system for monitoring and recording cell cultures. An improved lid (10) for the system includes pin sockets (28) interposed between traces (24) on a printed circuit board (22) and elongated, electrically conductive pins (38). A method for measuring a delay period for an alternating current voltage applied across a pair of pins (38) improves the stability of measurements made with the improved system. A method for applying a voltage across a pair of pins (38) which avoids exceeding a pre-established voltage avoids the possibility of damaging and/or destroying cells while monitoring their culture. Subtracting electrical conductivity measured for a reference well (16) that holds only cell growth media without cells from the electrical conductivity measured for other wells (16) during analysis of recorded data improves the presentation of test results obtained by monitoring and recording cell cultures.
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