公开(公告)号：EP0131418A1

公开(公告)日：1985-01-16

申请号：EP84304469.4

申请日：1984-06-29

申请人： CELLTECH LIMITED

发明人： Williamson, Robert ,  Carey, Norman Henry

IPC分类号： C12N15/00 ,  C12P21/02 ,  C12N9/20 ,  A61K37/54 ,  C12N1/16

CPC分类号： C12N9/20

摘要： @ A rat or human lingual lipase protein for use in the treatment of lipase deficiency. A process is described for producing lingual lipase using recombinant DNA technoloy to produce a host organism (for example E.coli) capable of producing a precursor of the lingual lipase which may be cleaved to yield the lingual lipase. The host organism is transformed with a vector including a gene coding for the precursor of lingual lipase. The precursor protein is for example prelingual lipase protein, or a fusion protein comprising lingual lipase and a heterologous protein. A pharmaceutical composition in unit dosage or liquid form is described.

摘要翻译： 用于治疗脂肪酶缺乏症的大鼠或人类舌酸脂肪酶蛋白质。 描述了使用重组DNA技术生产舌酸脂肪酶以制备能够产生舌状脂肪酶的前体的宿主生物体（例如大肠杆菌）的方法，其可被切割以产生舌头脂肪酶。 用包含编码舌脂肪酶前体的基因的载体转化宿主生物体。 前体蛋白质是例如舌前脂肪酶蛋白质，或包含舌头脂肪酶和异源蛋白质的融合蛋白质。 描述了单位剂量或液体形式的药物组合物。

公开(公告)号：EP0068691A3

公开(公告)日：1983-08-10

申请号：EP82303035

申请日：1982-06-11

申请人： CELLTECH LIMITED

发明人： Carey, Norman Henry ,  Doel, Michael Terence ,  Harris, Timothy John Roy ,  Lowe, Peter Anthony ,  Emtage, John Spencer

IPC分类号： C12N15/00 ,  C12N09/52 ,  C12N09/60 ,  C12N01/00 ,  C07H21/04 ,  C12Q01/38 ,  C12R01/19 ,  C12R01/865 ,  C12N09/64

CPC分类号： C12N9/6481 ,  C12N9/52 ,  C12N9/60 ,  C12N15/81 ,  C12Q1/37

摘要： A process is described for the production of the proteolytic enzyme chymosin. The process involves cleaving chymosin precursor polypeptides such as methionine-prochymosin, preprochymosin and methionine-chymosin expressed from a host organism which has been transformed with a vector system carrying the relevant gene. The gene sequence of preprochymosin is given. The vector systems used are plasmids adapted for the transformation of either bacterial or eukaryotic host organisms. E. Coli and saccharomyces cerevisiae are specific examples of suitable host organisms. There is also described an oligonucleotide capable of being used as a specific hybridisation probe or transcription primer for mRNA containing the nucleotide sequence coding for chymosin. A microassay for chymosin activity is also described.