-
公开(公告)号：EP0068691A3
公开(公告)日：1983-08-10
申请号：EP82303035
申请日：1982-06-11
申请人： CELLTECH LIMITED
发明人： Carey, Norman Henry , Doel, Michael Terence , Harris, Timothy John Roy , Lowe, Peter Anthony , Emtage, John Spencer
IPC分类号： C12N15/00 , C12N09/52 , C12N09/60 , C12N01/00 , C07H21/04 , C12Q01/38 , C12R01/19 , C12R01/865 , C12N09/64
CPC分类号： C12N9/6481 , C12N9/52 , C12N9/60 , C12N15/81 , C12Q1/37
摘要： A process is described for the production of the proteolytic enzyme chymosin. The process involves cleaving chymosin precursor polypeptides such as methionine-prochymosin, preprochymosin and methionine-chymosin expressed from a host organism which has been transformed with a vector system carrying the relevant gene. The gene sequence of preprochymosin is given. The vector systems used are plasmids adapted for the transformation of either bacterial or eukaryotic host organisms. E. Coli and saccharomyces cerevisiae are specific examples of suitable host organisms. There is also described an oligonucleotide capable of being used as a specific hybridisation probe or transcription primer for mRNA containing the nucleotide sequence coding for chymosin. A microassay for chymosin activity is also described.
-
公开(公告)号：EP0188312A2
公开(公告)日：1986-07-23
申请号：EP86300042.8
申请日：1986-01-06
申请人： CELLTECH LIMITED
发明人： Harris, Timothy John Roy , Docherty, Andrew James Penrose , Reynolds, John James , Murphy, Gillian
CPC分类号： C07K14/8146 , A61K38/00 , C07K2319/02
摘要： A process for producing a metalloproteinase inhibitor or a precursor thereof comprising culturing host cells transformed with a vector including a gene coding for the metalloproteinase inhibitor or the precursor and, if appropriate, cleaving the precursor to yield metalloproteinase inhibitor. The precursor may be a premetalloproteinase inhibitor or a fusion protein comprising a heterologous protein and a metalloproteinase inhibitor. The metalloproteinase inhibitor has application as the effective component in a pharmaceutical composition for the treatment of disease processes where accelerated breakdown of the extracellular organic matrix is observed.
摘要翻译： 一种生产金属蛋白酶抑制剂或其前体的方法，包括用包含编码金属蛋白酶抑制剂或前体的基因的载体转化宿主细胞，如果合适的话，切割前体以产生金属蛋白酶抑制剂。 前体可以是前金属蛋白酶抑制剂或包含异源蛋白质和金属蛋白酶抑制剂的融合蛋白。 金属蛋白酶抑制剂作为治疗疾病过程的药物组合物中有效成分的应用，其中观察到细胞外有机基质的加速分解。
-
公开(公告)号：EP0347078A1
公开(公告)日：1989-12-20
申请号：EP89305605.1
申请日：1989-06-02
申请人： CELLTECH LIMITED
IPC分类号： C12N9/64 , C12N15/00 , A61K37/547 , C12N5/00 , A61K39/395
CPC分类号： C12N9/6459 , A61K38/00 , C07K16/00 , C07K16/36 , C07K2319/31 , C07K2319/33 , C12Y304/21069
摘要： Recombinant DNA technology is employed to produce a single chain protein in which a Kringle domain and a serine protease domain of a plasminogen activator are linked to the heavy chain variable region of fibrin specific antibody. The single chain protein is associated with a complementary light chain variable domain of an antifibrin antibody so as to form a fibrin binding site. The construct has in vitro and in vivo clot lysis activity, the activity in vivo being similar to that of tPA, while fibrin binding is enhanced relative to tPA.
摘要翻译： 采用重组DNA技术产生单链蛋白，其中纤溶酶原激活物的Kringle结构域和丝氨酸蛋白酶结构域与纤维蛋白特异性抗体的重链可变区连接。 单链蛋白与抗纤维蛋白抗体的互补轻链可变结构域相关联，以形成纤维蛋白结合位点。 该构建体具有体外和体内凝块溶解活性，体内活性与tPA相似，而纤维蛋白结合相对于tPA增强。
-
公开(公告)号：EP0188312A3
公开(公告)日：1988-01-13
申请号：EP86300042
申请日：1986-01-06
申请人： CELLTECH LIMITED
发明人： Harris, Timothy John Roy , Docherty, Andrew James Penrose , Reynolds, John James , Murphy, Gillian
CPC分类号： C07K14/8146 , A61K38/00 , C07K2319/02
摘要： A process for producing a metalloproteinase inhibitor or a precursor thereof comprising culturing host cells transformed with a vector including a gene coding for the metalloproteinase inhibitor or the precursor and, if appropriate, cleaving the precursor to yield metalloproteinase inhibitor. The precursor may be a premetalloproteinase inhibitor or a fusion protein comprising a heterologous protein and a metalloproteinase inhibitor. The metalloproteinase inhibitor has application as the effective component in a pharmaceutical composition for the treatment of disease processes where accelerated breakdown of the extracellular organic matrix is observed.
-
-
-
搜索结果
4 条记录 (耗时 0.008 秒)
