摘要:
The present invention relates to a method for separating the components of a mixture M by electrophoresis in a single capillary, wherein: (A) the compounds of the mixture M are introduced and migrate inside the capillary according to the capillary electrophoresis technique; (B) a fraction F of the compounds separated in step (A) is isolated in the capillary and said isolated fraction F is forced to migrate towards the vicinity of one end of the capillary while maintaining this fraction inside the capillary; (C) upon the application of a pressure difference between the ends of the capillary, a second separation medium MS2 different from the first separation medium MS1 of step (A) is introduced into the capillary through the end of the capillary in the vicinity of which the fraction F is located, whereby the fraction F migrates to the other end of the capillary while filling the capillary with the separation medium MS2; and (D) an capillary electrophoresis is carried out in the capillary thus filled with the separation medium MS2, using the medium MS2 as the separation medium.
摘要:
This method comprises steps consisting in: injecting (100) a sample into a capillary tube; conveying (110) the sample along the capillary tube, under experimental conditions able to generate a Taylor dispersion effect; generating (120) a signal characterising the Taylor dispersion; processing (130) said signal to obtain the experimental Taylor signal Ŝ ( t ); and analysing (200) said experimental Taylor signal Ŝ(t) . The analyzing step consists in obtaining an amplitude distribution P(G (c) ) allowing said experimental Taylor signal Ŝ (t) to be decomposed into a sum of Gaussian functions by implementing a constraint-solving algorithm consisting in minimising a cost function H a comprising at least one constraint term associated with a constraint that the amplitude distribution P(G (c) ) must respect, this minimisation being carried out over a range of values of interest of a parameter G (c) characterising the Gaussian amplitude function P(G (c) ) .
摘要:
The subject of the present invention is a method for determining the hydrodynamic range of the constituents of a mixture, in which: (A) by capillary electrophoresis, the constituents of the mixture M are separated, leaving them within the capillary; (B) at one of the ends of the capillary thus obtained, containing, in various zones, the constituents separated in step (A), a marker is injected that can be detected by a detection device placed on the side facing the other end of the capillary; (C) a pressure difference is induced between the ends of the capillary so as to make the various constituents separated during step (A) and, finally, the marker migrate towards the outlet of the capillary; and (D) the hydrodynamic range of each of said constituents is determined, by analysing the Taylor dispersion produced in step (C), from the time at which the marker is detected and from the elution profile of each of the constituents.
摘要:
The present invention relates to a method for separating the components of a mixture M by electrophoresis in a single capillary, wherein: (A) the compounds of the mixture M are introduced and migrate inside the capillary according to the capillary electrophoresis technique; (B) a fraction F of the compounds separated in step (A) is isolated in the capillary and said isolated fraction F is forced to migrate towards the vicinity of one end of the capillary while maintaining this fraction inside the capillary; (C) upon the application of a pressure difference between the ends of the capillary, a second separation medium MS2 different from the first separation medium MS1 of step (A) is introduced into the capillary through the end of the capillary in the vicinity of which the fraction F is located, whereby the fraction F migrates to the other end of the capillary while filling the capillary with the separation medium MS2; and (D) an capillary electrophoresis is carried out in the capillary thus filled with the separation medium MS2, using the medium MS2 as the separation medium.