公开(公告)号：EP0060045A3

公开(公告)日：1983-08-24

申请号：EP82300865

申请日：1982-02-22

申请人： CETUS CORPORATION

发明人： GELFAND, DAVID HARROW

IPC分类号： C12N15/09 ,  C07H21/04 ,  C07K14/005 ,  C07K14/195 ,  C12N1/00 ,  C12N15/00 ,  C12N15/69 ,  C12N15/70 ,  C12P21/00 ,  C12P21/02 ,  C12P21/04

CPC分类号： C12N15/70 ,  C12N15/69

摘要： The problem of producing stable DNA plasmids of high copy number may be solved by selecting mutants in which an altered repressor gene leads to high copy number replication. The plasmids are modifed by placing a suitable transcriptional "stop" signal after an inserted heterogenous gene so that the readthrough expression of the heterologous DNA will not continue into the replication primer strand. Deletions resulting from interference with replication primer strand transcription are thereby avoided.
Such plasmids may be used to produce a desired protein by introducing them into a host microorganism and allowing the host to express phenotypically its genotype (including the plasmids).

摘要翻译： 通过选择其中改变的阻遏物基因导致高拷贝数复制的突变体，可以解决产生高拷贝数的稳定DNA质粒的问题。 通过在插入的异源基因之后放置合适的转录“停止”信号来修饰质粒，使得异源DNA的读取表达不会继续进入复制引物链。 从而避免了由于干扰复制引物链转录而导致的缺失。 这样的质粒可用于通过将其引入宿主微生物并使宿主表达其基因型（包括质粒）来产生所需的蛋白质。