公开(公告)号：EP0226419A3

公开(公告)日：1988-04-20

申请号：EP86309516

申请日：1986-12-05

申请人： CETUS CORPORATION ,  THE GOVERNMENT OF THE UNITED STATES OF AMERICA as represented by the Secretary Department of Commerce

发明人： Bjorn, Michael Jon ,  Fitzgerald, David ,  Frankel, Arthur Edward ,  Laird, Walter Joseph ,  Pastan, Ira ,  Ring, David Barratt ,  Willingham, Mark ,  Winkelhake, Jeffrey

IPC分类号： A61K47/00 ,  A61K39/395 ,  A61K37/02

CPC分类号： C07K16/30 ,  A61K38/00 ,  A61K47/6869 ,  Y10S424/804 ,  Y10S424/807 ,  Y10S514/885 ,  Y10S530/808 ,  Y10S530/864

摘要： Immunotoxins comprising a cytotoxic moiety and monoclonal antibodies which bind to human ovarian cancer tissue having at least one of the following capabilities are claimed: cytotoxic ID 50 of 10 nM or less against human ovarian cancer cells, retardation of human ovarian cancer tumor growth in mammals or extension of survival of a mammal carrying a human ovarian cancer tumor. Antigens to which the monoclonal antibody of the immunotoxin bind are identified and characterize the immunotoxins. Methods of killing human ovarian cancer cells, retarding the growth of human ovarian cancer tumors in mammals or extending the survival of mammals carrying human ovarian cancer tumors are claimed.

公开(公告)号：EP0226418A3

公开(公告)日：1988-04-27

申请号：EP86309515

申请日：1986-12-05

申请人： CETUS CORPORATION

发明人： Bjorn, Michael Jon ,  Frankel, Arthur Edward ,  Laird, Walter Joseph ,  Ring, David Barratt ,  Winkelhake, Jeffrey Lee

IPC分类号： A61K47/00 ,  A61K37/02 ,  A61K35/74

CPC分类号： C07K16/30 ,  A61K38/00 ,  A61K47/6869

摘要： Immunotoxins comprising a cytotoxic moiety and an antigen binding portion selected from the group consisting of Fab, Fab' and F(ab') 2 fragments of a monoclonal antibody, which binds to human ovarian cancer tissue, having one of the following capabilities are claimed: cytotoxic ID 50 of about 10 nM or less against human ovarian cancer cells, retardation of human ovarian cancer tumor growth in mammals, or extension of survival of a mammal carrying a human ovarian cancer tumor. Antigens or epitopes to which the monoclonal antibodies bind are identified and characterize the immunotoxins. In a preferred embodiment an immunotoxin comprising at least an antigen binding portion of a monoclonal antibody, which binds to human transferrin receptor, but does not block binding of transferrin to the receptor, is described and claimed. Immunotoxin comprising the (F(ab') 2 region of the antitransferrin monoclonal antibody are also claimed. Methods of killing human ovarian cancer cells, retarding the growth of human ovarian tumors in mammals and extending the survival of mammals carrying human ovarian tumors are claimed.

摘要翻译： 要求保护包含细胞毒性部分和选自Fab，Fab'和F（ab'）2单克隆抗体片段的抗原结合部分的免疫毒素，其结合人卵巢癌组织，具有以下能力之一： 对人卵巢癌细胞的约10nM或更低的细胞毒性ID50，在哺乳动物中延迟人卵巢癌肿瘤生长或携带人卵巢癌肿瘤的哺乳动物的存活延长。 鉴定单克隆抗体结合的抗原或表位，并表征免疫毒素。 在一个优选实施方案中，描述并要求保护包含至少一个单克隆抗体的抗原结合部分的免疫毒素，其结合人转铁蛋白受体但不阻断转铁蛋白与受体的结合。 要求保护包含抗转铁蛋白单克隆抗体（F（ab'）2区域的免疫毒素。）要求保护杀死人卵巢癌细胞，延缓哺乳动物中人卵巢肿瘤生长和延长携带人卵巢肿瘤的哺乳动物存活的方法。