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    PROCESS FOR PRODUCING FOREIGN PROTEIN IN ESCHERICHIA COLI
    1.
    发明公开
    PROCESS FOR PRODUCING FOREIGN PROTEIN IN ESCHERICHIA COLI 有权
    过程在大肠杆菌中产生杂质蛋白

    公开(公告)号：EP1602720A4

    公开(公告)日：2008-06-18

    申请号：EP04713206

    申请日：2004-02-20

    申请人： CHEMO SERO THERAPEUT RES INST

    发明人： KOYANAGI SATOSHI ,  SUGAWARA KEISHIN ,  MIYATSU YOSHINOBU ,  MURAKAMI TOSHIO ,  MAEDA TOSHIHIRO ,  MIZOKAMI HIROSHI

    IPC分类号： C12N15/70 ,  C12P21/02 ,  C12N15/09 ,  C07K14/435

    CPC分类号： C07K14/43531 ,  C12N15/70 ,  C12P21/02

    摘要： It is intended to provide a foreign protein free from any expression inducer and a process for producing the same. Namely, a process for producing a foreign protein involving the step of proliferating a recombinant Escherichia coli, which expresses the foreign protein under the control by a promoter capable of inducing the expression due to the temperature shift, by arbitrarily culturing at a low temperature and then culturing the strain at a high temperature in the absence of an expression inducer to thereby allow the expression of the foreign protein, or the step of culturing the above Escherichia coli strain at a high temperature and thus effecting the proliferation of the cells and the expression of the foreign protein at the same time; and a foreign protein free from any expression inducer which is obtained by this process. Particular examples of such foreign proteins include main tick allergens, Actinogacillus pleuropneumoniae-origin secretory macrophage toxin and a protective antigen against Erysipelothrix rhusiopathiae-infection.

    METHOD OF PURIFYING MODIFIED ACARIAN MAIN ALLERGEN
    2.
    发明公开
    METHOD OF PURIFYING MODIFIED ACARIAN MAIN ALLERGEN 有权
    清理方法的螨更改的主过敏原

    公开(公告)号：EP1621615A4

    公开(公告)日：2007-05-02

    申请号：EP04727416

    申请日：2004-04-14

    申请人： CHEMO SERO THERAPEUT RES INST

    发明人： KOYANAGI SATOSHI ,  KAWATSU KENJIRO ,  MURAKAMI TOSHIO ,  MIYATSU YOSHINOBU ,  MAEDA TOSHIHIRO ,  MIZOKAMI HIROSHI

    IPC分类号： C12N15/09 ,  A61K35/64 ,  A61K38/17 ,  A61P37/08 ,  C07K14/435

    CPC分类号： C07K14/43531 ,  A61K38/00 ,  A61K39/00

    摘要： A method of purifying a modified acarian main allergen obtained by a technology of genetic manipulation; and a modified acarian main allergen having been purified by the purification method. In particular, a method of purifying a modified acarian main allergen obtained by a technology of genetic manipulation, which method is characterized by comprising the steps of (1) with the use of an MF membrane, washing and recovering an inclusion body containing a modified acarian main allergen obtained by a technology of genetic manipulation, (2) dissolving the inclusion body and performing refolding, (3) with the use of an ultrafiltration membrane, concentrating the resultant modified acarian main allergen containing solution and removing low-molecular components, (4) with the use of an anion exchanger, recovering the modified acarian main allergen as a non-adsorbed fraction, (5) with the use of hydrophobic gel, recovering the modified acarian main allergen as an adsorbed fraction and (6) with the use of an anion exchanger, recovering the modified acarian main allergen as an adsorbed fraction; and a modified acarian main allergen of high purity obtained by the purification method.