NOVEL GREEN AND ORANGE FLUORESCENT LABELS AND THEIR USES
    1.
    发明公开
    NOVEL GREEN AND ORANGE FLUORESCENT LABELS AND THEIR USES 审中-公开
    奥古斯丁绿色荧光灯

    公开(公告)号:EP1546673A4

    公开(公告)日:2006-08-09

    申请号:EP03754878

    申请日:2003-09-23

    申请人: CHROMAGEN INC

    摘要: The present invention provides novel fluorescent compounds and covalent attachment chemistries which facilitate the use of these compounds as labels for ultrasensitive and quantitative fluorescent detection of low levels of biomolecules. In a preferred embodiment, the fluorescent labels of this invention are novel derivatives of the hydroxy-pyrene trisulphonic and disulphonic acids which may be used in any assay in which radioisotopes, colored dyes or other fluorescent molecules are currently used. Thus, for example, any assay using labeled antibodies, proteins, oligonucleotides or lipids, including fluorescent cell sorting, fluorescence microscopy (including dark-field microscopy), fluorescence polarization assays, ligand, receptor binding assays, receptor activation assays and diagnostic assays can benefit from use of the compounds disclosed herein.

    摘要翻译: 本发明提供了新颖的荧光化合物和共价附着化学物质,其有利于这些化合物用作低水平生物分子的超敏感和定量荧光检测的标签。 在优选的实施方案中,本发明的荧光标记是羟基芘三磺酸和二磺酸的新衍生物,其可用于目前使用放射​​性同位素,着色染料或其它荧光分子的任何测定中。 因此,例如,使用标记的抗体,蛋白质,寡核苷酸或脂质(包括荧光细胞分选,荧光显微术(包括暗视野显微镜),荧光偏振测定,配体,受体结合测定,受体活化测定和诊断测定) 使用本文公开的化合物。