公开(公告)号：EP2202307A4

公开(公告)日：2012-01-25

申请号：EP08838661

申请日：2008-10-14

申请人： CHUGAI PHARMACEUTICAL CO LTD

发明人： YAMADA YOSHIKI ,  KIYASU TATSUYA ,  TABATA KAZUYUKI ,  KUZUMAKI AKIHIRO ,  YAMASHIRO MAI

IPC分类号： C12N15/09 ,  C12N5/10 ,  C12P21/02

CPC分类号： C07K16/00 ,  C07K2317/56 ,  C12N2510/02

摘要： The present invention provides a method of producing a recombinant antibody efficiently and at low cost. Disclosed is a method of producing an antibody or a fragment thereof, comprising allowing a cell to produce the antibody or the fragment, wherein the cell contains a larger number of copies of an exogenous DNA encoding the light chain or a fragment thereof of the antibody than the number of copies contained in the cell of an exogenous DNA encoding the heavy chain or a fragment thereof of the antibody. The present invention also provides a recombinant vector comprising one copy of a DNA encoding the heavy chain or a fragment thereof of an antibody and two or more copies of a DNA encoding the light chain or a fragment thereof of the antibody; and a transformant.