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1.发明授权COUPLED POLYMERASE CHAIN REACTION-RESTRICTION ENDONUCLEASE DIGESTION-LIGASE DETECTION REACTION PROCESS 有权PROCEDURE ON聚合酶链反应的偶联反应（PCR）-VERDAUUNG用限制性酶和连接酶基于
公开(公告)号：EP1165838B1
公开(公告)日：2006-10-11
申请号：EP00918081.1
申请日：2000-03-17
IPC分类号： C12Q1/68
CPC分类号： C12Q1/683 , C12Q1/6816 , C12Q1/6853 , C12Q2565/125 , C12Q2563/179 , C12Q2531/137 , C12Q2565/501 , C12Q2537/149 , C12Q2535/125 , C12Q2525/155
摘要： The present invention provides a method for identifying one or more low abundance sequences differing by one or more single-base changes, insertions, or deletions, from a high abundance sequence in a plurality of target nucleotide sequences. The high abundance wild-type sequence is selectively removed using high fidelity polymerase chain reaction analog conversion, facilitated by optimal buffer conditions, to create a restriction endonuclease site in the high abundance wild-type gene, but not in the low abundance mutant gene. This allows for digestion of the high abundance DNA. Subsequently the low abundant mutant DNA is amplified and detected by the ligase detection reaction assay. The present invention also relates to a kit for carrying out this procedure.
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2.发明公开HIGH FIDELITY DETECTION OF NUCLEIC ACID DIFFERENCES BY LIGASE DETECTION REACTION 失效NUCLEIC差异，通过连接酶检测反应的高灵敏度检测
公开(公告)号：EP0956359A1
公开(公告)日：1999-11-17
申请号：EP97936059.0
申请日：1997-07-15
CPC分类号： C12Q1/6827 , C07K14/82 , C12N9/93 , C12Q1/6837 , C12Q1/6862 , C12Q2531/113 , C12Q2561/125 , C12Q2565/501
摘要： Ligase detection reaction is utilized to distinguish minority template in the presence of an excess of normal template with a thermostable ligase. This process can be carried out with a mutant ligase, thermostable ligase, or a modified oligonucleotide probe. This procedure is particularly useful for the detection of cancer-associated mutations. It has the advantage of providing a quantitative measure of the amount or ratio of minority template.
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3.发明授权HIGH FIDELITY DETECTION OF NUCLEIC ACID DIFFERENCES BY LIGASE DETECTION REACTION 失效NUCLEIC差异，通过连接酶检测反应的高灵敏度检测
公开(公告)号：EP0956359B1
公开(公告)日：2011-10-26
申请号：EP97936059.1
申请日：1997-07-15
CPC分类号： C12Q1/6827 , C07K14/82 , C12N9/93 , C12Q1/6837 , C12Q1/6862 , C12Q2531/113 , C12Q2561/125 , C12Q2565/501
摘要： Ligase detection reaction is utilized to distinguish minority template in the presence of an excess of normal template with a thermostable ligase. This process can be carried out with a mutant ligase, thermostable ligase, or a modified oligonucleotide probe. This procedure is particularly useful for the detection of cancer-associated mutations. It has the advantage of providing a quantitative measure of the amount or ratio of minority template.
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4.发明公开COUPLED POLYMERASE CHAIN REACTION-RESTRICTION ENDONUCLEASE DIGESTION-LIGASE DETECTION REACTION PROCESS 有权PROCEDURE ON聚合酶链反应的偶联反应（PCR）-VERDAUUNG用限制性酶和连接酶基于
公开(公告)号：EP1165838A2
公开(公告)日：2002-01-02
申请号：EP00918081.1
申请日：2000-03-17
IPC分类号： C12Q1/68
CPC分类号： C12Q1/683 , C12Q1/6816 , C12Q1/6853 , C12Q2565/125 , C12Q2563/179 , C12Q2531/137 , C12Q2565/501 , C12Q2537/149 , C12Q2535/125 , C12Q2525/155
摘要： The present invention provides a method for identifying one or more low abundance sequences differing by one or more single-base changes, insertions, or deletions, from a high abundance sequence in a plurality of target nucleotide sequences. The high abundance wild-type sequence is selectively removed using high fidelity polymerase chain reaction analog conversion, facilitated by optimal buffer conditions, to create a restriction endonuclease site in the high abundance wild-type gene, but not in the low abundance mutant gene. This allows for digestion of the high abundance DNA. Subsequently the low abundant mutant DNA is amplified and detected by the ligase detection reaction assay. The present invention also relates to a kit for carrying out this procedure.
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