公开(公告)号：EP0840800A4

公开(公告)日：1999-10-06

申请号：EP96920165

申请日：1996-05-09

申请人： CURAGEN CORP

发明人： SIMPSON JOHN W ,  ROTHBERG JONATHAN M ,  WENT GREGORY T

IPC分类号： B01J19/00 ,  G01N27/447 ,  C12Q1/68 ,  B01D61/42 ,  B01D61/44 ,  C07H21/02 ,  C07H21/04 ,  C12P19/34 ,  C12Q1/70 ,  C25B1/00 ,  C25B7/00 ,  C25D13/00 ,  G01N27/26

CPC分类号： B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00873 ,  B01J2219/00995 ,  G01N27/44708 ,  G01N27/44721 ,  G01N27/44782 ,  G01N27/44791 ,  Y10S436/80 ,  Y10T436/143333

摘要： This invention is an integrated instrument for the high capacity electrophoretic analysis of biopolymer samples. It comprises a specialized high-voltage, electrophoretic module in which migration lanes are formed between a bottom plate (446) and a plurality of etched grooves in a top plate (438), the module permitting concurrent separation of 80 or more separate samples. In thermal contact with the bottom plate (446) is a thermal control module incorporating a plurality of Peltier heat transfer devices for the control of temperature and gradients in the electrophoretic medium. Fragments are detected by a transmission imaging spectrograph which simultaneously spatially focuses and spectrally resolves the detection region of all the migration lanes. The spectrograph comprises a transmission dispersion element and a CCD array to detect signals. Signal analysis comprises the steps of noise filtering, comparison in a configuration space with signal prototypes, and selection of the best prototype. Optionally post-processing is done by a Monte-Carlo simulated annealing algorithm to improve results. Optionally, an array of micro-reactors can be integrated into the instrument for the generation of sequencing reaction fragments directly from crude DNA samples.

公开(公告)号：EP1147409A4

公开(公告)日：2002-09-11

申请号：EP99966079

申请日：1999-12-09

申请人： CURAGEN CORP

发明人： BADER JOEL S ,  ROTHBERG JONATHAN M ,  DEEM MICHAEL W ,  MULHERN GREGORY T ,  WENT GREGORY T ,  SIMPSON JOHN ,  HENCK STEVEN

IPC分类号： B01D57/02 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N27/26 ,  G01N27/447 ,  G01N33/483 ,  G01N33/50 ,  G01N37/00

CPC分类号： B82Y30/00 ,  G01N27/44773

摘要： This invention relates to a method and device for separating particles according to their diffusivities in a separation medium by means of a spatially and temporally varying electric potential. The method takes advantage of the transport of charged particles subject to an electric potential that is cycled between an off-state (in which the potential is flat) and one or more on-states, in which the potential is preferably spatially periodic with a plurality of eccentrically shaped stationary wells. The potential wells are at a constant spatial positions in the on-state. Differences in liquid-phase diffusivities lead to charged particle separation. A separation medium fills physically defined separation lanes (15) in the device. Electrodes deposited substantially transverse to the lanes create the required potential. Advantageously, injection ports (16) allow sample loading, and special gating electrodes focus the sample prior to separation.

公开(公告)号：EP0990141A4

公开(公告)日：2001-07-25

申请号：EP97925527

申请日：1997-05-12

申请人： CURAGEN CORP

发明人： JARVIE THOMAS P ,  BADER JOEL S ,  WENT GREGORY T

IPC分类号： G01R33/32 ,  G01N24/08 ,  G01N33/483 ,  G01R33/46 ,  G01N24/12 ,  G01N33/53 ,  G06F19/00 ,  G06F159/00

CPC分类号： G01R33/4625 ,  G01R33/4641 ,  Y10T436/24

摘要： This invention includes methods for analyzing data generated by various solid-state NMR experiments, including rotational echo double resonance (REDOR), transferred echo double resonance (TEDOR), dipolar recoupling at the magic angle (DRAMA), dipolar recoupling with a windowless sequence (DRAWS), and melding of spin-locking and DRAMA (MELODRAMA). The methods are based alternately on a new analytical transform or the maximum entropy method and their multidimensional extensions. They permit simultaneous, multiple distance measurements of high accuracy and precision, even from nuclei with identical chemical shifts. By providing high quality easily obtained distance measurement from disordered solid state materials, this invention also improves drug discovery and design through fast determination of structures of pharmaceutical lead compounds, drug molecules, or their targets.

公开(公告)号：EP0972187A4

公开(公告)日：2000-01-19

申请号：EP97917713

申请日：1997-03-26

申请人： CURAGEN CORP

发明人： BADER JOEL S ,  ROTHBERG JONATHAN M ,  DEEM MICHAEL W ,  MULHERN GREGORY T ,  WENT GREGORY T

IPC分类号： B01D57/02 ,  G01N27/26 ,  G01N27/447

CPC分类号： B82Y30/00 ,  G01N27/44773

摘要： A device for separating charged particles comprising: an upper substate (11); a lower substrate (12); separation lanes (15) defined on the upper substrate, a first plurality of electrodes (21, 22...); a first pad (18), said first plurality of electrodes connected to said first pad (13); a second plurality of electrodes (21, 23...); a second pad (14), said second plurality of electrodes connected to said second pad; said pads and electrodes deposited on the lower substrate; and said first plurality of electrodes and said second plurality of electrodes are interdigitated. During the operation of the device, charged particles are subjected to an electric potential that is cycled between an off-state and one or more on states, in which the potential is preferably spatially periodic with a plurality of eccentrically shaped stationary potential wells.

摘要翻译： 一种用于分离带电粒子的装置，包括：上子状件（11）; 下基板（12）; 限定在上基板上的分离道（15），第一多个电极（21,22 ...）; 第一焊盘（18），所述第一多个电极连接到所述第一焊盘（13）; 第二多个电极（21,23 ...）; 第二焊盘（14），所述第二多个电极连接到所述第二焊盘; 所述焊盘和电极沉积在下基板上; 并且所述第一多个电极和所述第二多个电极是交叉指状的。 在装置操作期间，带电粒子经受在断态和一个或多个导通状态之间循环的电势，其中电势优选地在空间上具有多个偏心形状的静态势阱的周期性周期。