公开(公告)号：EP1231470A1

公开(公告)日：2002-08-14

申请号：EP00971755.4

申请日：2000-11-02

申请人： Center for Advanced Science and Technology Incubation, Ltd.

发明人： KARUBE, Isao ,  IKEBUKURO, Kazunori ,  YANO, Kazuyoshi ,  MIYACHI, Hirotaka

IPC分类号： G01N33/543 ,  G01N33/547

CPC分类号： G01N33/544 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/0063 ,  B01J2219/00637 ,  C12Q1/6837 ,  G01N33/54353 ,  G01N33/54393 ,  C12Q2565/501 ,  C12Q2565/531

摘要： A method for immobilizing substances by formation of plasma-polymerized films, after the arrangement of the substances to be immobilized on the surface of a support, and the immobilized substances according to the method are provided. The immobilized substances are retained with use of plasma-polymerized films, thereby damage to the immobilized substances is minimized, and the liberation of the substances after the immobilization will hardly occur. They can be further applied for indirect immobilization of biotinylated polynucleotides, because binding activities of the substances are kept on the surface. In addition, proteins having binding activities such as antibodies can be immobilized to be used for the detection of ligands.

摘要翻译： 提供通过形成等离子体聚合膜来固定物质的方法，在待固定在载体表面上的物质的排列和根据该方法的固定化物质之后。 使用等离子体聚合膜保留固定化物质，使固定化物质的损伤减到最小，固定化后的物质的释放几乎不发生。 它们可以进一步应用于生物素化多核苷酸的间接固定，因为物质的结合活性保留在表面上。 此外，具有结合活性的蛋白质如抗体可以被固定以用于检测配体。