公开(公告)号：EP2189539B2

公开(公告)日：2018-06-13

申请号：EP09175511.6

申请日：2009-11-10

申请人： Chimera Biotec GmbH

发明人： Adler, Michael ,  Detmers, Jan

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6804 ,  C12Q1/682 ,  C12Q2565/531 ,  C12Q2563/179 ,  C12Q2563/131

摘要： The present invention relates to novel conjugate complexes for immunoassays as well as kits comprising these conjugate complexes, methods of producing these complexes, and methods of detecting an analyte by use of these complexes. The conjugate complexes of the invention comprise one or more non-nucleic acid receptors capable of specifically binding an analyte, one or more nucleic acid markers comprising a predetermined nucleotide sequence, one or more first linker molecules capable of specifically binding the non-nucleic acid receptor and the nucleic acid marker, and one or more second linker molecules capable of specifically binding the first linker molecules.

公开(公告)号：EP3295161A2

公开(公告)日：2018-03-21

申请号：EP16797122

申请日：2016-05-16

申请人： TWO PORE GUYS INC

发明人： MORIN TREVOR J ,  HELLER DANIEL A ,  DUNBAR WILLIAM B

IPC分类号： G01N27/447 ,  G01N33/483 ,  G01N33/487

CPC分类号： C12Q1/6816 ,  C12Q2563/155 ,  C12Q2565/531 ,  C12Q2565/631

摘要： Provided are methods and compositions for detecting a target analyte suspected to be present in a sample with background molecules using a nanopore device. A plurality of probes for polymer scaffold identification or for target analyte binding and detection are provided.

公开(公告)号：EP3154439A1

公开(公告)日：2017-04-19

申请号：EP16725089.3

申请日：2016-05-19

申请人： Yaya Diagnostics GmbH

发明人： DYCK, Joachim ,  LENK, Jens ,  SAUERMANN, Stefan ,  PÖHLMANN, Tobias

IPC分类号： A61B10/00 ,  C12Q1/68

CPC分类号： C12Q1/6806 ,  A61B10/0045 ,  C12Q2527/146 ,  C12Q2565/518 ,  C12Q2565/519 ,  C12Q2565/531

摘要： The present invention relates to means for and methods of collecting, enriching, and analyzing nucleic acids of interest of interest, e.g. those that are associated with the development of sepsis. These means may be used in vivo or in vitro using obtained nucleic acids of interest, e.g. for liquid biopsies. The invention can, inter alia, be used in the detection and enrichment as well as analysis of nucleic acids of interests such as those derived from pathogens or from individuals that may contain such pathogens.

摘要翻译： 本发明涉及收集，丰富和分析感兴趣的核酸的方法和方法，例如， 那些与败血症发展相关的那些。 这些方法可以在体内或体外使用获得的感兴趣的核酸，例如， 用于液体活检。 本发明特别可以用于检测和富集以及分析感兴趣的核酸，例如衍生自病原体的那些或可能含有这种病原体的个体。

公开(公告)号：EP2844766A1

公开(公告)日：2015-03-11

申请号：EP13724179.0

申请日：2013-04-29

申请人： Qiagen GmbH

发明人： WEDLER, Holger ,  WEDLER, Erika ,  LÖFFERT, Dirk ,  O'NEIL, Dominic

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q2525/191 ,  C12Q2563/131 ,  C12Q2563/143 ,  C12Q2565/531

摘要： The invention relates to a method for enriching one or more target sequences of a deoxyribonucleic acid (DNA) in a composition, comprising the steps of providing a composition comprising one or more deoxyribonucleic acid (DNA) molecules, hybridizing to said one or more DNA molecules, one or more target specific ribonucleic acid (RNA) hybridization probes, thereby forming one or more RNA/DNA hybrids, capturing the RNA/DNA hybrids with one or more antibodies being specific for such RNA/DNA hybrids, thereby forming one or more RNA/DNA/antibody hybrids, isolating the one or more RNA/DNA/antibody hybrids, amplifying the one or more DNA molecules of the one or more RNA/DNA/antibody hybrids if necessary, and, optionally, sequencing the one or more DNA molecules of the one or more RNA/DNA/antibody hybrids or the amplification product, wherein the sequencing is preferably done by means of next generation sequencing. The invention also relates to a kit comprising a first an antibody which is specific for a DNA/RNA hybrid molecule, wherein optionally the antibody is bound to a magnetic particle, and additionally comprising one or more target specific RNA hybridization probes.

公开(公告)号：EP2189539B1

公开(公告)日：2011-09-07

申请号：EP09175511.6

申请日：2009-11-10

申请人： Chimera Biotec GmbH

发明人： Adler, Michael ,  Detmers, Jan

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6804 ,  C12Q1/682 ,  C12Q2565/531 ,  C12Q2563/179 ,  C12Q2563/131

摘要： The present invention relates to novel conjugate complexes for immunoassays as well as kits comprising these conjugate complexes, methods of producing these complexes, and methods of detecting an analyte by use of these complexes. The conjugate complexes of the invention comprise one or more non-nucleic acid receptors capable of specifically binding an analyte, one or more nucleic acid markers comprising a predetermined nucleotide sequence, one or more first linker molecules capable of specifically binding the non-nucleic acid receptor and the nucleic acid marker, and one or more second linker molecules capable of specifically binding the first linker molecules.

公开(公告)号：EP2208795A4

公开(公告)日：2011-02-02

申请号：EP08831240

申请日：2008-08-27

申请人： KANEKA CORP

发明人： TOMONO JUN

IPC分类号： C12Q1/68 ,  C12M1/00 ,  C12N15/09 ,  G01N33/53 ,  G01N33/566 ,  G01N33/58

CPC分类号： G01N33/5308 ,  C12Q1/6844 ,  G01N33/566 ,  C12Q2565/113 ,  C12Q2563/107 ,  C12Q2565/625 ,  C12Q2565/137 ,  C12Q2565/531

摘要： In the present invention, an amplified DNA fragment having a first substance binding site to which a first substance is specifically bindable is prepared, which amplified DNA fragment amplified by a nucleic acid amplification method. The amplified DNA fragment is concentrated by binding the amplified DNA fragment to the first substance. The concentration makes it possible to detect the DNA highly sensitively. Therefore, with the arrangement, it is possible to detect the amplified DNA fragment amplified by the nucleic acid amplification method, easily and highly accurately without requiring any special device.

公开(公告)号：EP1412752A4

公开(公告)日：2004-09-29

申请号：EP02747993

申请日：2002-06-28

申请人： VERI Q INC ,  PROTEOME SCIENCES PLC

发明人： PEARCE CHRISTOPHER D J ,  MITCHELL LLOYD G

IPC分类号： C07K16/44 ,  C12M1/00 ,  C12M1/34 ,  C12N15/09 ,  C12P19/34 ,  C12Q1/68 ,  C40B40/06 ,  C40B50/14 ,  G01N33/53 ,  G01N33/543 ,  G01N33/566 ,  G01N33/58 ,  G01N37/00 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04

CPC分类号： B82Y30/00 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00675 ,  B01J2219/00693 ,  B01J2219/00722 ,  C07K16/44 ,  C12P19/34 ,  C12Q1/6804 ,  C12Q1/6837 ,  C40B40/06 ,  C40B50/14 ,  G01N33/5308 ,  G01N33/543 ,  G01N2500/00 ,  Y02P20/55 ,  C12Q2565/531 ,  C12Q2563/131

摘要： An antibody microarray is described comprising a plurality of antibodies immobilized on a substrate, wherein each antibody specifically binds to a synthetic oligomer (e.g., an oligonucleotide or oligopeptide) having an organic protecting group covalently bound thereto, which antibody does not bind to that synthetic oligomer when the organic protecting group is not covalently bound thereto. Methods of making and using such antibodies are disclosed, along with cells for making such antibodies. Methods of making and using such antibody microarrays are also disclosed.

摘要翻译： 描述了包含固定在底物上的多种抗体的抗体微阵列，其中每种抗体特异性结合具有与其共价结合的有机保护基团的合成寡聚体（例如，寡核苷酸或寡肽），该抗体不结合该合成寡聚体 当有机保护基团不与其共价结合时。 公开了制备和使用这些抗体的方法，以及用于制备这种抗体的细胞。 还公开了制造和使用这种抗体微阵列的方法。

公开(公告)号：EP1203093A4

公开(公告)日：2003-03-19

申请号：EP00939737

申请日：2000-06-09

申请人： GILEAD SCIENCES INC

发明人： DROLET DANIEL ,  GOLD LARRY

IPC分类号： G01N33/53 ,  C12N15/09 ,  C12N15/113 ,  C12Q1/68 ,  G01N33/566 ,  C07H19/00 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  C12P19/34

CPC分类号： C12N15/1137 ,  C12N2310/18 ,  C12N2310/322 ,  C12Q1/6811 ,  C12Q2565/531 ,  C12Q2523/313

摘要： Methods are described for the identification and preparation of nucleic acid ligands to calf intestinal phosphatase. Included in the invention are specific RNA ligands to calf intestinal phosphatase identified by the SELEX method.

公开(公告)号：EP1231470A1

公开(公告)日：2002-08-14

申请号：EP00971755.4

申请日：2000-11-02

申请人： Center for Advanced Science and Technology Incubation, Ltd.

发明人： KARUBE, Isao ,  IKEBUKURO, Kazunori ,  YANO, Kazuyoshi ,  MIYACHI, Hirotaka

IPC分类号： G01N33/543 ,  G01N33/547

CPC分类号： G01N33/544 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/0063 ,  B01J2219/00637 ,  C12Q1/6837 ,  G01N33/54353 ,  G01N33/54393 ,  C12Q2565/501 ,  C12Q2565/531

摘要： A method for immobilizing substances by formation of plasma-polymerized films, after the arrangement of the substances to be immobilized on the surface of a support, and the immobilized substances according to the method are provided. The immobilized substances are retained with use of plasma-polymerized films, thereby damage to the immobilized substances is minimized, and the liberation of the substances after the immobilization will hardly occur. They can be further applied for indirect immobilization of biotinylated polynucleotides, because binding activities of the substances are kept on the surface. In addition, proteins having binding activities such as antibodies can be immobilized to be used for the detection of ligands.

摘要翻译： 提供通过形成等离子体聚合膜来固定物质的方法，在待固定在载体表面上的物质的排列和根据该方法的固定化物质之后。 使用等离子体聚合膜保留固定化物质，使固定化物质的损伤减到最小，固定化后的物质的释放几乎不发生。 它们可以进一步应用于生物素化多核苷酸的间接固定，因为物质的结合活性保留在表面上。 此外，具有结合活性的蛋白质如抗体可以被固定以用于检测配体。

公开(公告)号：EP3307773A1

公开(公告)日：2018-04-18

申请号：EP16812371.9

申请日：2016-06-15

申请人： Ubiome, Inc.

发明人： APTE, Zachary ,  ALMONACID, Daniel ,  RICHMAN, Jessica

IPC分类号： C07K16/00 ,  G01N33/532 ,  C12Q1/68 ,  C12P19/34 ,  C12N9/96 ,  A61K39/00

CPC分类号： C12Q1/6869 ,  C12Q1/6804 ,  G01N33/58 ,  C12Q2525/179 ,  C12Q2565/531

摘要： A method and system for characterization of antibody-bound targets by sequencing of synthetic oligonucleotides bound to the antibodies, the method including: receiving a sample having a set of antibodies; conjugating each of the set of antibodies with an oligonucleotide, thereby generating a set of oligonucleotide-conjugated antibodies; binding a first subset of the set of oligonucleotide-conjugated antibodies to a set of targets at a capture substrate; determining a sequence for at least one of: 1) each oligonucleotide of the first subset of oligonucleotide-conjugated antibodies that bind to the set of targets and 2) each oligonucleotide of a second subset of the set of oligonucleotide-conjugated antibodies that fail to bind to the set of targets; and generating an analysis of the sample from the sequences determined from at least one of the first and the second subsets of oligonucleotide-conjugated antibodies.