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    PROCEDE DE DETECTION D'UNE ESPECE FLUORESCENTE REVERSIBLEMENT PHOTOCONVERTIBLE
    4.
    发明公开
    PROCEDE DE DETECTION D'UNE ESPECE FLUORESCENTE REVERSIBLEMENT PHOTOCONVERTIBLE 有权
    用于检测的照片可切换荧光材料相使用方法偏移成像光学调制

    公开(公告)号：EP3071948A1

    公开(公告)日：2016-09-28

    申请号：EP14802429.2

    申请日：2014-11-21

    申请人： Centre National de la Recherche Scientifique ,  Institut Curie ,  Ecole Normale Supérieure de Paris ,  Université Pierre et Marie Curie

    发明人： JULLIEN, Ludovic ,  LE SAUX, Thomas ,  GAUTIER, Arnaud ,  CROQUETTE, Vincent ,  SARANG, Nath ,  WANG, Pencheng ,  QUERARD, Jérôme ,  ALBRIGHT, Samantha

    IPC分类号： G01N21/64

    CPC分类号： G01N21/6408 ,  A61K49/0017 ,  G01N21/6428 ,  G01N21/6458 ,  G01N33/5306 ,  G01N2201/12

    摘要： Method for detection of at least one reversibly photo-convertible fluorescent species (P), comprising the following steps: a) illumination of a sample comprising said or at least one of said reversibly photo-convertible fluorescent species by a periodically modulated illuminating light (FEX); and b) detection of fluorescent radiation (FLU) emitted by the sample thus illuminated; characterised in that the method further comprises the following step: c) extraction of the amplitude (lF0Ut) of the intensity component of said fluorescent radiation exhibiting the same periodicity as said periodically modulated illuminating light and a phase quadrature with respect to the same; and in that the mean intensity of said illuminating light and the modulation frequency of the same are chosen in such a way as to maximise said amplitude of the intensity component of said fluorescent radiation.

    摘要翻译： 用于检测至少一种可逆的光转换的荧光物质（P），包括以下步骤的方法：1）包含所述或所述的至少一个通过一个周期性调制照明光可逆地光电转换的荧光物质的样品的照度（FEX ）; 和b）检测通过这样照射的样品发射的荧光发射（FLU）的; 其特征在于DASS模法还包括在所述荧光发射的强度成分的振幅（IFOUT）表现出相同的周期性，所述周期性调制的照明光，并相对于相同的一个相位正交的下面步骤c）的提取; 并且在没有所述照明光的平均强度和相同的调制频率在寻求一种方式，以最大化所述荧光发射的强度分量的所述幅度被选择。

    DETERMINATION DES RAYONS HYDRODYNAMIQUES DES CONSTITUANTS D'UN MELANGE PAR ANALYSE D'UNE DISPERSION DE TAYLOR EFFECTUEE SUITE A UNE SEPARATION PAR ELECTROPHORESE CAPILLAIRE
    8.
    发明公开
    DETERMINATION DES RAYONS HYDRODYNAMIQUES DES CONSTITUANTS D'UN MELANGE PAR ANALYSE D'UNE DISPERSION DE TAYLOR EFFECTUEE SUITE A UNE SEPARATION PAR ELECTROPHORESE CAPILLAIRE 有权
    分析混合物的成分流体力学领域毛细管分离判定造成TAYLOR色散

    公开(公告)号：EP2067017A2

    公开(公告)日：2009-06-10

    申请号：EP07848238.7

    申请日：2007-09-14

    申请人： CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS)

    发明人： COTTET, Hervé ,  LE SAUX, Thomas

    IPC分类号： G01N15/02 ,  B01F13/00

    CPC分类号： G01N27/447 ,  G01N27/44726

    摘要： The subject of the present invention is a method for determining the hydrodynamic range of the constituents of a mixture, in which: (A) by capillary electrophoresis, the constituents of the mixture M are separated, leaving them within the capillary; (B) at one of the ends of the capillary thus obtained, containing, in various zones, the constituents separated in step (A), a marker is injected that can be detected by a detection device placed on the side facing the other end of the capillary; (C) a pressure difference is induced between the ends of the capillary so as to make the various constituents separated during step (A) and, finally, the marker migrate towards the outlet of the capillary; and (D) the hydrodynamic range of each of said constituents is determined, by analysing the Taylor dispersion produced in step (C), from the time at which the marker is detected and from the elution profile of each of the constituents.