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1.发明授权MEANS AND METHODS FOR MODIFYING GENE EXPRESSION USING UNPOLYADENYLATED RNA 有权手段与方法的变革基因表达的非聚腺苷酸化RNA
公开(公告)号：EP1208211B1
公开(公告)日：2007-11-14
申请号：EP00953352.2
申请日：2000-08-14
发明人： WANG, Ming-Bo , WATERHOUSE, Peter
CPC分类号： C12N15/8218 , C12N9/2405 , C12N15/8216 , C12N15/8247 , C12N15/8283 , C12Y302/01031
摘要： Methods and means are provided for reducing expression of a nucleic acid of interest, thereby altering the phenotype of an organism, particularly a plant, by providing aberrant, preferably unpolyadenylated, target specific RNA to the nucleus of the host cell. Preferably, the unpolyadenylated, target-specific RNA is provided by expresssion of a chimeric DNA construct comprising a promoter, a DNA region encoding the target specific RNA, a self-splicing ribozyme and a DNA region involved in 3' end formation and polyadenylation.
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公开(公告)号：EP1068311A1
公开(公告)日：2001-01-17
申请号：EP99910592.7
申请日：1999-04-07
CPC分类号： C12N15/8283 , C12N15/63 , C12N15/8218 , C12N15/8247 , C12N15/8282
摘要： Methods and means are provided for reducing the phenotypic expression of a nucleic acid of interest in eucaryotic cells, particularly in plant cells, by introducing chimeric genes encoding sense and antisense RNA molecules directed towards the target nucleic acid, which are capable of forming a double stranded RNA region by base-pairing between the regions with the sense and antisense nucleotide sequence or by introducing the RNA molecules themselves. Preferably, the RNA molecules comprises simultaneously both sense and antisense nucleotide sequence.
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公开(公告)号：EP3214177A3
公开(公告)日：2017-11-22
申请号：EP17162147.7
申请日：1999-04-07
CPC分类号： C12N15/8283 , C12N15/63 , C12N15/8218 , C12N15/8247 , C12N15/8282
摘要： Methods and means are provided for reducing the phenotypic expression of a nucleic acid of interest in eukaryotic cells, particularly plant cells, by introducing a chimeric RNA molecule comprising at least one RNA region with a nucleotide sequence comprising
i. a sense nucleotide sequence of at least 15 consecutive nucleotides having 100% sequence identity with at least part of the nucleotide sequence of the nucleic acid of interest; and
ii. an antisense nucleotide sequence including at least 15 consecutive nucleotides, having about 100% sequence identity with the complement of said at least 15 consecutive nucleotides of said sense nucleotide sequence;
wherein said RNA region is capable of forming an artificial hairpin RNA structure with a double stranded RNA stem by base-pairing between the regions with sense and antisense nucleotide sequence such that said at least 15 consecutive nucleotides of the sense sequence basepair with said at least 15 consecutive nucleotides of the antisense sequence, wherein said nucleic acid of interest is a gene integrated into the genome of said eukaryotic cell, wherein said RNA molecule is produced by transcription of a chimeric gene.摘要翻译： 提供了用于通过将包含至少一个RNA区的嵌合RNA分子与包含i的核苷酸序列导入来减少真核细胞，特别是植物细胞中感兴趣的核酸的表型表达的方法和手段。 与感兴趣的核酸的核苷酸序列的至少一部分具有100％序列同一性的至少15个连续核苷酸的正义核苷酸序列; 和ii。 反义核苷酸序列，其包含至少15个连续核苷酸，与所述有义核苷酸序列的所述至少15个连续核苷酸的互补序列具有约100％的序列同一性; 其中所述RNA区域能够通过具有有义和反义核苷酸序列的区域之间的碱基配对形成具有双链RNA干扰的人造发夹RNA结构，使得有义序列碱基对的所述至少15个连续核苷酸与所述至少15个 其中所述感兴趣的核酸是整合到所述真核细胞的基因组中的基因，其中所述RNA分子通过嵌合基因的转录产生。
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公开(公告)号：EP3214177A2
公开(公告)日：2017-09-06
申请号：EP17162147.7
申请日：1999-04-07
CPC分类号： C12N15/8283 , C12N15/63 , C12N15/8218 , C12N15/8247 , C12N15/8282
摘要： Methods and means are provided for reducing the phenotypic expression of a nucleic acid of interest in eukaryotic cells, particularly plant cells, by introducing a chimeric RNA molecule comprising at least one RNA region with a nucleotide sequence comprising
i. a sense nucleotide sequence of at least 15 consecutive nucleotides having 100% sequence identity with at least part of the nucleotide sequence of the nucleic acid of interest; and
ii. an antisense nucleotide sequence including at least 15 consecutive nucleotides, having about 100% sequence identity with the complement of said at least 15 consecutive nucleotides of said sense nucleotide sequence;
wherein said RNA region is capable of forming an artificial hairpin RNA structure with a double stranded RNA stem by base-pairing between the regions with sense and antisense nucleotide sequence such that said at least 15 consecutive nucleotides of the sense sequence basepair with said at least 15 consecutive nucleotides of the antisense sequence, wherein said nucleic acid of interest is a gene integrated into the genome of said eukaryotic cell, wherein said RNA molecule is produced by transcription of a chimeric gene.-
公开(公告)号：EP1068311B1
公开(公告)日：2011-04-27
申请号：EP99910592.7
申请日：1999-04-07
CPC分类号： C12N15/8283 , C12N15/63 , C12N15/8218 , C12N15/8247 , C12N15/8282
摘要： Methods and means are provided for reducing the phenotypic expression of a nucleic acid of interest in eucaryotic cells, particularly in plant cells, by introducing chimeric genes encoding sense and antisense RNA molecules directed towards the target nucleic acid, which are capable of forming a double stranded RNA region by base-pairing between the regions with the sense and antisense nucleotide sequence or by introducing the RNA molecules themselves. Preferably, the RNA molecules comprises simultaneously both sense and antisense nucleotide sequence.
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6.发明公开MEANS AND METHODS FOR MODIFYING GENE EXPRESSION USING UNPOLYADENYLATED RNA 有权手段与方法的变革基因表达的非聚腺苷酸化RNA
公开(公告)号：EP1208211A1
公开(公告)日：2002-05-29
申请号：EP00953352.2
申请日：2000-08-14
发明人： WANG, Ming-Bo , WATERHOUSE, Peter
CPC分类号： C12N15/8218 , C12N9/2405 , C12N15/8216 , C12N15/8247 , C12N15/8283 , C12Y302/01031
摘要： Methods and means are provided for reducing expression of a nucleic acid of interest, thereby altering the phenotype of an organism, particularly a plant, by providing aberrant, preferably unpolyadenylated, target specific RNA to the nucleus of the host cell. Preferably, the unpolyadenylated, target-specific RNA is provided by expresssion of a chimeric DNA construct comprising a promoter, a DNA region encoding the target specific RNA, a self-splicing ribozyme and a DNA region involved in 3' end formation and polyadenylation.
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公开(公告)号：EP1068311B2
公开(公告)日：2020-12-09
申请号：EP99910592.7
申请日：1999-04-07
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公开(公告)号：EP2185706B1
公开(公告)日：2017-09-27
申请号：EP08782928.9
申请日：2008-08-14
发明人： WANG, Ming-Bo , WATERHOUSE, Peter
CPC分类号： C12N15/113 , A61K31/713 , C12N15/111 , C12N15/63 , C12N2310/14 , C12N2320/50 , C12N2330/30
摘要： Provided are methods and means to obtain improved gene silencing of target nucleic acids whereby at least two inhibitory RNA molecules are provided which are targeted to the same nucleic acid, but which are processed into short interfering RNA molecules through different processing pathways. Also provided are methods and means to obtain improved gene silencing of target nucleic acids whereby at least two inhibitory RNA molecules are provided which are targeted to different nucleic acids, but which are processed into short interfering RNA molecules through different processing pathways.
摘要翻译： 提供了获得靶核酸的改进的基因沉默的方法和手段，由此提供至少两种靶向相同核酸但是通过不同处理途径加工成短干扰RNA分子的抑制性RNA分子。 还提供了获得靶核酸的改进的基因沉默的方法和手段，由此提供至少两种抑制性RNA分子，其靶向不同的核酸，但通过不同的加工途径加工成短干扰RNA分子。
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公开(公告)号：EP2185706A1
公开(公告)日：2010-05-19
申请号：EP08782928.9
申请日：2008-08-14
发明人： WANG, Ming-Bo , WATERHOUSE, Peter
CPC分类号： C12N15/113 , A61K31/713 , C12N15/111 , C12N15/63 , C12N2310/14 , C12N2320/50 , C12N2330/30
摘要： Provided are methods and means to obtain improved gene silencing of target nucleic acids whereby at least two inhibitory RNA molecules are provided which are targeted to the same nucleic acid, but which are processed into short interfering RNA molecules through different processing pathways. Also provided are methods and means to obtain improved gene silencing of target nucleic acids whereby at least two inhibitory RNA molecules are provided which are targeted to different nucleic acids, but which are processed into short interfering RNA molecules through different processing pathways.
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公开(公告)号：EP2018420A1
公开(公告)日：2009-01-28
申请号：EP07718830.8
申请日：2007-05-03
发明人： WATERHOUSE, Peter , WANG, Ming-Bo
CPC分类号： C12N15/8218
摘要： Methods and means are provided to modulate gene silencing in eukaryotes through the alteration of the functional level of particular DICER or DICER like proteins. Also provided are methods and means to modulate post-transcriptional gene silencing in eukaryotes through the alteration of the functional level of proteins involved in transcriptional silencing of the silencing RNA encoding genes.
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