公开(公告)号：EP2519625B1

公开(公告)日：2018-09-26

申请号：EP10841651.2

申请日：2010-12-28

Applicant: DSM IP Assets B.V.

Inventor： LIPPMEIER, James Casey ,  APT, Kirk E. ,  HANSEN, Jon Milton

IPC: C12N1/10

CPC classification number: C12N15/79 ,  A23K20/10 ,  A23K50/80 ,  A23L2/52 ,  A23L33/10 ,  A23V2002/00 ,  C12N1/10 ,  C12N1/12 ,  C12N9/2405 ,  C12P7/6463 ,  C12P7/649 ,  C12Y302/01026 ,  C12Y302/01048 ,  Y02E50/13

Abstract: The present invention is directed to recombinant thraustochytrids that grow on sucrose and cell cultures comprising the recombinant thraustochytrids as well as methods of producing cell cultures, biomasses, microbial oils, compositions, and biofuels using the recombinant thraustochytrids.

公开(公告)号：EP2885407A4

公开(公告)日：2016-05-11

申请号：EP13829902

申请日：2013-08-15

Applicant: BANGLADESH JUTE RES INST

Inventor： ALAM MAQSUDUL ,  ISLAM MOHAMMED SHAHIDUL ,  HOSSEN MOHAMMED MOSADDEQUE ,  HAQUE MOHAMMED SAMIUL ,  ALAM MOHAMMED MONJURUL

IPC: C12N9/88

CPC classification number: C12N9/88 ,  C12N9/18 ,  C12N9/2405 ,  C12Y301/01011 ,  C12Y402/02002 ,  C12Y402/02023

Abstract: The present invention discloses isolated polynucleotide encoding enzymes, derived from the fungus Macrophomina phaseolina (“M. phaseolina”), responsible for degrading pectin, and it comprises and/or consists of nucleotide sequences set forth in SEQ ID Nos. 2, 5, 8, 11, 14, 17, 20, 23, 26, 29, 32, 35, 38, 41, 44, 47, 50, 53, 56, 59, 62, 1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58 and 61, or the complement of such sequences. The present invention also relates to isolated polypeptide encoded by the polynucleotide sequences set forth in SEQ ID Nos. 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 60 and 63; a recombinant gene construct comprising the polynucleotide; a transformant and a transgenic fungus comprising the recombinant gene construct, with or having enhanced production of pectin degrading enzyme. The polypeptide of the invention can be used for, amongst other things, manufactured fruit juice, textile products, pulp and paper, coffee, tea and oil extraction and pectic waste water treatment.

公开(公告)号：EP3004366A2

公开(公告)日：2016-04-13

申请号：EP14727821.2

申请日：2014-06-02

Applicant: DSM IP Assets B.V.

Inventor： BOER, Viktor, Marius ,  SUIR, Erwin

IPC: C12P15/00 ,  C12P19/56 ,  A23L2/60 ,  C12N9/90 ,  C12N9/88 ,  C12N9/02 ,  C12N9/16 ,  C12N9/24 ,  C12N9/10 ,  C07K14/39 ,  C07K14/395 ,  C12N15/52

CPC classification number: C12P19/56 ,  A23L2/60 ,  A23L27/36 ,  A23V2002/00 ,  C07H15/256 ,  C07K14/39 ,  C07K14/395 ,  C12N9/0036 ,  C12N9/0042 ,  C12N9/0073 ,  C12N9/0083 ,  C12N9/1051 ,  C12N9/16 ,  C12N9/2405 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12P15/00

Abstract: The present invention relates to a recombinant microorganism comprising one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity, whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol. The recombinant microorganism may also be capable of expressing one or more UDP-glucosyltransferases such that the microorganism is capable of producing one or more steviol glycosides.

Abstract translation: 本发明涉及包含一个或多个核苷酸序列的重组微生物，所述核苷酸序列编码：具有入 - copalyl焦磷酸合成酶活性的多肽; 具有ent-Kaurene合酶活性的多肽; 具有内皮氧化酶活性的多肽; 和具有海藻酸13-羟化酶活性的多肽，由此所述核苷酸序列的表达赋予所述微生物至少产生甜菊醇的能力。 重组微生物还可能能够表达一种或多种UDP-葡糖基转移酶，使得微生物能够产生一种或多种甜菊糖苷。

公开(公告)号：EP2699667B1

公开(公告)日：2016-04-13

申请号：EP12717560.2

申请日：2012-04-20

Applicant: Danisco US Inc.

Inventor： BODIE, Elizabeth A. ,  PRATT II, Robert James

IPC: C12N1/14 ,  C12N15/80 ,  C12R1/885 ,  C07K14/37

CPC classification number: C12N15/79 ,  C12N1/14 ,  C12N1/36 ,  C12N9/2405 ,  C12N15/80

Abstract: Described are compositions and methods relating to variant filamentous fungi having altered growth characteristics. Such variants are well-suited for growth in submerged cultures, e.g., for the large-scale production of enzymes and other proteins for commercial applications.

公开(公告)号：EP2817325A1

公开(公告)日：2014-12-31

申请号：EP13751987.2

申请日：2013-02-19

Applicant: Novozymes Biopolymer A/S

Inventor： SPODSBERG, Nikolaj ,  ANDERSON, Lars

IPC: C07K14/37 ,  C12N9/46 ,  C12N15/56 ,  C12N15/63 ,  C12N1/11

CPC classification number: C12N9/2405

Abstract: Provided are isolated polypeptides having endoglucanase activity, catalytic domains, cellulose binding domains and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides catalytic domains or cellulose binding domains.

公开(公告)号：EP2444490B1

公开(公告)日：2014-02-12

申请号：EP11007478.8

申请日：2006-12-08

Applicant: Verenium Corporation ,  BP Corporation North America Inc.

Inventor： Gray, Kevin A. ,  Zhao, Lishan ,  Cayouette, Michelle H.

IPC: C12N9/42

CPC classification number: C12N9/2437 ,  C12N9/2402 ,  C12N9/2405 ,  C12N9/248 ,  C12Y302/01004 ,  C12Y302/01006 ,  C12Y302/01008 ,  C12Y302/01021 ,  C12Y302/01023 ,  C12Y302/01039 ,  C12Y302/01055 ,  C12Y302/01091 ,  Y02E50/16 ,  Y02E50/17 ,  Y10T428/249921

公开(公告)号：EP1766601B8

公开(公告)日：2013-11-06

申请号：EP05761074.3

申请日：2005-06-20

Applicant: CP Kelco U.S., Inc.

Inventor： CLEARY, Joseph, M. ,  COLEMAN, Russell, J. ,  HARDING, Nancy, E. ,  PATEL, Yamini, N.

IPC: C12P19/04 ,  C12N9/16 ,  C12N9/24 ,  A23L1/054 ,  C08B37/00 ,  C12N1/21 ,  C12N15/55 ,  C12N15/56

CPC classification number: C08L5/00 ,  A23C9/137 ,  A23C9/1544 ,  A23L29/272 ,  C08B37/006 ,  C12N9/16 ,  C12N9/2402 ,  C12N9/2405 ,  C12P19/04 ,  C12Y302/01031

公开(公告)号：EP2655637A1

公开(公告)日：2013-10-30

申请号：EP11808093.6

申请日：2011-12-20

Applicant: The Board of Trustees of the University of Illinois

Inventor： HAN, Yejun ,  MOON, Young, Hwan ,  SU, Xaioyun ,  YOSHIDA, Shosuke ,  MIYAGI, Atsushi ,  DODD, Dylan ,  MACKIE, Roderick, I. ,  CANN, Isaac, K.O.

IPC: C12P1/04 ,  C07K14/195

CPC classification number: C12P19/14 ,  C12N9/18 ,  C12N9/2402 ,  C12N9/2405 ,  C12N9/2434 ,  C12N9/248 ,  C12N9/2482 ,  C12P7/04 ,  C12P7/14 ,  C12P7/16 ,  C12P19/02 ,  C12Y301/01072 ,  C12Y302/01008 ,  C12Y302/01055 ,  C12Y302/01072 ,  C12Y302/01139

Abstract: The disclosure provides thermostable enzymes isolated from Caldicellulosiruptor bescii and fragments thereof useful for the degradation of cellulose and/or hemicellulose, including thermostable cellulases and hemicellulases. The disclosure further provides nucleic acids encoding the thermostable enzymes of the disclosure. The disclosure also provides methods for the conversion of cellulose and hemicellulose into fermentable sugars using thermostable enzymes of the disclosure. The disclosure also provides enzyme cocktails containing multiple enzymes disclosed herein. The enzymes can be used to release sugars present in cellulose or hemicellulose for subsequent fermentation to produce value-added products.

Abstract translation: 本公开提供了从Caldicellulosiruptor bescii分离的热稳定酶及其片段，其可用于纤维素和/或半纤维素的降解，包括耐热纤维素酶和半纤维素酶。 本公开进一步提供了编码本公开的热稳定酶的核酸。 本公开还提供了使用本公开的热稳定酶将纤维素和半纤维素转化为可发酵糖的方法。 本公开还提供了含有本文公开的多种酶的酶混合物。 酶可用于释放存在于纤维素或半纤维素中的糖，用于随后的发酵以产生增值产物。

公开(公告)号：EP2612866A2

公开(公告)日：2013-07-10

申请号：EP11822043.3

申请日：2011-07-21

Applicant: Biotopia Co., Ltd.

Inventor： SEONG, Su-il ,  HWANG, Kyo Yeol ,  LEE, Jae Yeon ,  KANG, Kyung-don ,  PARK, Young Shik ,  CHO, Yong Seok ,  YUCK, Won Jeong

IPC: C07K14/32 ,  C12N15/31 ,  C12N15/63 ,  C12P21/04

CPC classification number: C07K14/32 ,  C12N9/0004 ,  C12N9/1096 ,  C12N9/16 ,  C12N9/2405 ,  C12N15/52 ,  C12P17/12 ,  C12Y206/01019 ,  C12Y302/01084

Abstract: The present invention relates to a polypeptide associated with the biosynthesis of 1-deoxynojirimycin which is a substance that inhibits α-glucosidase activity, to a polynucleotide coding therefor, to a vector comprising the polynucleotide, to a transformant comprising the vector, and to a method in which the transformant is used in order to produce either a polypeptide associated with the synthesis of 1-deoxynojirimycin or 1-deoxynojirimycin. The polynucleotide of the present invention can be used in the volume production of 1-deoxynojirimycin, or can be used in studies searching for novel 1-deoxynojirimycin derivatives using recombinant technology, and hence is useful in developing pathogenic virus inhibitors and hypoglycemic agents for diabetic patients.

Abstract translation: 本发明涉及与含有该多核苷酸的载体的编码该多核苷酸的1-脱氧野尻霉素（其为抑制β-葡萄糖苷酶活性的生物合成）与包含该载体的转化体相关的多肽，以及一种方法 其中使用转化体以产生与1-脱氧野尻霉素或1-脱氧野尻霉素合成相关的多肽。 本发明的多核苷酸可用于1-脱氧野尻霉素的批量生产，或可用于使用重组技术研究新型1-脱氧野尻霉素衍生物的研究，因此可用于开发糖尿病患者的病原性病毒抑制剂和降血糖药 。

公开(公告)号：EP2519625A1

公开(公告)日：2012-11-07

申请号：EP10841651.2

申请日：2010-12-28

Applicant: DSM IP Assets B.V.

Inventor： LIPPMEIER, James Casey ,  APT, Kirk E. ,  HANSEN, Jon Milton

IPC: C12N1/10

CPC classification number: C12N15/79 ,  A23K20/10 ,  A23K50/80 ,  A23L2/52 ,  A23L33/10 ,  A23V2002/00 ,  C12N1/10 ,  C12N1/12 ,  C12N9/2405 ,  C12P7/6463 ,  C12P7/649 ,  C12Y302/01026 ,  C12Y302/01048 ,  Y02E50/13

Abstract: The present invention is directed to recombinant thraustochytrids that grow on sucrose and cell cultures comprising the recombinant thraustochytrids as well as methods of producing cell cultures, biomasses, microbial oils, compositions, and biofuels using the recombinant thraustochytrids.