公开(公告)号：EP2743678A1

公开(公告)日：2014-06-18

申请号：EP12821746.0

申请日：2012-08-07

申请人： Consejo Superior De Investigaciones Científicas (CSIC) ,  Instituto de Salud Carlos III ,  Fundación Cien ,  Fundación Para La Investigación Biomédica Del Hospital 12 de Octubre

发明人： CARMONA HERNÁNDEZ, Pedro ,  TOLEDANO GASCA, Adolfo ,  CALERO LARA, Miguel ,  MARTÍNEZ MARTÍN, Pablo ,  BERMEJO PAREJA, Félix

IPC分类号： G01N21/35

CPC分类号： G01N33/49 ,  G01N21/3577 ,  G01N2800/2821

摘要： The present invention relates to a method for transmission infrared spectroscopic analysis for determining the degree of global cognitive impairment associated with Alzheimer's disease based on analyzing the 3100-1300 cm -1 infrared spectrum region of a previously obtained and fractionated sample of blood. Preferably, the fractionated sample of blood to be analyzed is a fraction of leukocytes.
Likewise, another object of the present invention relates to the use of said method to obtain an indication of the status of the Alzheimer's disease and/or an indication of the degree of cognitive impairment associated with said disease.

摘要翻译： 本发明涉及一种用于为确定性采矿与阿尔茨海默氏病相关的全球认知损害的基础上分析该3100-1300 -1的血液中的以前取得并分级的样品的红外光谱区中的传输程度红外光谱分析的方法。 优选地，待分析的血液中的分级的样品是白细胞的一小部分。 同样地，本发明的另一个目的涉及使用所述方法来获得上的阿耳茨海默氏病和/或与所述疾病有关的认知损害的程度的指示状态指示。

公开(公告)号：EP2885625A1

公开(公告)日：2015-06-24

申请号：EP13756001.7

申请日：2013-08-20

申请人： Consejo Superior De Investigaciones Científicas (CSIC) ,  Fundación Investigación Biomédica Hospital Universitario 12 de Octubre ,  Instituto de Salud Carlos III ,  Fundación Cien ,  Universidad Complutense De Madrid

发明人： CARMONA HERNÁNDEZ, Pedro ,  TOLEDANO GASCA, Adolfo ,  CALERO LARA, Miguel ,  MARTÍNEZ MARTÍN, Pablo ,  BERMEJO PAREJA, Félix ,  MOLINA SANTOS, Marina

IPC分类号： G01N21/35 ,  G01N21/65

CPC分类号： G01N21/3577 ,  G01N21/65 ,  G01N33/49 ,  G01N2201/06113 ,  G01N2800/2821

摘要： The present invention relates to a Raman spectroscopy method for determining protein structure associated with global cognitive deterioration in Alzheimer's disease from the Raman spectroscopic analysis of a human blood sample, preferably of a human blood plasma sample, comprising obtaining at least one spectral value of at least one of the Raman spectrum regions comprised between 1600-1700 cm -1 , between 910-980 cm -1 , between 730-760 cm -1 and/or between 390-450 cm -1 , and where said spectral value allows obtaining an indication of the presence or absence of protein structure associated with a condition of Alzheimer's disease. The present invention also relates to a method for the infrared spectroscopic analysis of a blood sample, by means of additionally obtaining at least one spectral value of at least one of the infrared spectrum regions comprised between 1600 and 1700 cm -1 and/or between 1000 and 1150 cm -1 . Optionally, the Raman spectroscopy method further comprises the infrared spectroscopic analysis of the same blood sample. The invention also relates to a diagnostic method for diagnosing Alzheimer's disease comprising measuring a Raman spectrum and/or an infrared spectrum of a plasma sample.

摘要翻译： 本发明涉及用于从人血液样品的拉曼光谱分析中阿尔茨海默氏病与全球认知退化相关联的确定性采矿蛋白质结构的拉曼光谱的方法，优选的是人血浆样品，其包括获得至少一个频谱值至少 1600-1700 -1之间由拉曼光谱区域中的一个，910-980 -1之间，730-760 -1之间和/或390至450厘米-1，并且其中所述频谱值允许获得指示之间 的蛋白质结构与阿尔茨海默氏病的病症相关的存在或不存在。 因此，本发明涉及一种用于血液样品，的红外分光分析的方法通过获得另外1600和1700 -1之间和/或1000之间由红外光谱区的至少一个中的至少一个频谱值的平均值 和1150 -1。 任选地，该拉曼光谱方法还包括将同一血液样品的红外光谱分析。 因此，本发明涉及用于诊断阿尔茨海默病包括测量的拉曼光谱和/或血浆样品的红外光谱的诊断方法。