公开(公告)号：EP1299705A2

公开(公告)日：2003-04-09

申请号：EP01950638.5

申请日：2001-06-28

申请人： Coulter International Corp.

发明人： BURSHTEYN, Alexander ,  JOUBRAN, John, W. ,  KUYLEN, Nazle ,  LUCAS, Frank, J.

IPC分类号： G01N1/34

CPC分类号： G01N1/4077 ,  B01D61/147 ,  B01D61/18 ,  B01D61/20 ,  B01D63/02 ,  Y10T436/25375 ,  Y10T436/255

摘要： A method for utilizing a filtration device (24) for removing interferants from a sample containing cells (20) in an automated apparatus (10) is disclosed. The filtration device includes a microporous hollow fiber membrane (60) having a plurality of pores (65) sized to retain cells while allowing smaller diameter interferants (72) to pass through the membrane. The apparatus also includes a means of moving the sample from a sample container (16) to and from the filtration device. The disclosed method utilizes a vacuum source (30) to aspirate the sample into a lumen (66) of the hollow fiber membrane so that the sample is retained in the lumen space until expelled into an analysis container or transported to an analyzer.

公开(公告)号：EP0948639B1

公开(公告)日：2005-08-10

申请号：EP97913899.7

申请日：1997-10-29

申请人： Coulter International Corp.

发明人： KUYLEN, Nazle ,  CREWS, Harold, R. ,  JAFFE, Gerald, E. ,  BARCELON, Maria ,  LUCAS, Francis, J. ,  GARCIA, Nancy, M.

IPC分类号： C12Q1/00 ,  C12Q1/04 ,  C12Q1/26 ,  C12Q1/37 ,  C12Q1/44 ,  C12Q1/42 ,  C12Q1/34 ,  G01N15/14 ,  G01N33/50 ,  G01N21/64

CPC分类号： C12Q1/34 ,  C12Q1/26 ,  C12Q1/37 ,  G01N33/574 ,  G01N33/582 ,  G01N2333/95 ,  G01N2333/96413 ,  G01N2333/96466 ,  G01N2333/96469 ,  G01N2800/52

公开(公告)号：EP0948639A1

公开(公告)日：1999-10-13

申请号：EP97913899.0

申请日：1997-10-29

申请人： Coulter International Corp.

发明人： KUYLEN, Nazle ,  CREWS, Harold, R. ,  JAFFE, Gerald, E. ,  BARCELON, Maria ,  LUCAS, Francis, J. ,  GARCIA, Nancy, M.

IPC分类号： G01N33 ,  C12Q1

CPC分类号： C12Q1/34 ,  C12Q1/26 ,  C12Q1/37 ,  G01N33/574 ,  G01N33/582 ,  G01N2333/95 ,  G01N2333/96413 ,  G01N2333/96466 ,  G01N2333/96469 ,  G01N2800/52

摘要： The necessity to detect blast cells in a patient sample to determine a treatment regime for leukemia has long been desired. The present invention relates to a method and assay reagents for identification of blast cells in a fluid sample. The method further enables the determination of the lineage of the blast cells. The new method provides a determination of blast cells in less time than prior art methods.