公开(公告)号：EP1494752B1

公开(公告)日：2008-07-30

申请号：EP03719469.3

申请日：2003-04-11

申请人： Cyto Pulse Sciences, Inc.

发明人： WALTERS, Richard, E. ,  KING, Alan, D. ,  DEBRUIN, Katherine, A.

IPC分类号： A61N1/05

CPC分类号： A61N1/0412 ,  A61N1/0476 ,  A61N1/306 ,  A61N1/325 ,  A61N1/327

摘要： A method and apparatus are provided for treating biological cellular material with a treating agent using pulsed electrical fields provided by a waveform generator (12). The treatment method includes obtaining an electrode assembly which includes three or more parallel rows of individual electrodes (19). The electrode assembly is applied to a treatment area. Electrically conductive pathways are established between the electrodes (19) and the waveform generator (12) through an array switch (14). Successive electric fields are applied to the treatment area in the form of successive electric field waveforms from the waveform generator (12), through the array switch (14), to adjacent rows of electrodes (19), wherein each successive electric field has the same direction, and wherein polarities of rows of electrodes are reversed successively during the applying of the successive electric fields between adjacent successive rows of electrodes to the treatment area. As a result, the biological cellular material in the treatment area is treated with the treating agent unidirectionally with uniform electric fields with a minimization of the formation of deleterious electrochemistry products at the electrodes.

公开(公告)号：EP2160201A1

公开(公告)日：2010-03-10

申请号：EP08767820.7

申请日：2008-05-20

申请人： Cyto Pulse Sciences, Inc.

发明人： WALTERS, Richard, E. ,  WALTERS, Derin, C. ,  KING, Alan, D. ,  ROOS, Anna-Karin

IPC分类号： A61K48/00 ,  A61N1/30

CPC分类号： A61N1/327 ,  A61K39/12 ,  A61K2039/53 ,  A61K2039/54 ,  A61N1/306 ,  C07K14/005 ,  C12N2770/24122 ,  C12N2770/24134

摘要： An object of the invention is to provide a method and apparatus for the delivery of polynucleotide vaccines into mammalian skin cells to increase T cell response and reduce pain and discomfort due to long electric waveform application and due to muscle contractions. The method includes administering a polynucleotide vaccine into the skin at an administration site, (b ) applying a needle electrode to the skin in the vicinity to the administration site, and (c ) applying a sequence of at least three single, operator-controlled, independently programmed, narrow interval electrical waveforms, which have pulse intervals that are less than 100 milliseconds, to deliver the 15 polynucleotide vaccine into the skin cells by electroporation.

公开(公告)号：EP1472358B1

公开(公告)日：2008-05-07

申请号：EP02723493.9

申请日：2002-04-05

申请人： Cyto Pulse Sciences, Inc.

发明人： WALTERS, Richard, E. ,  WALTERS, Derin, C. ,  KING, Alan, D.

IPC分类号： C12N15/87

CPC分类号： C12M35/02 ,  A61K2039/5152 ,  A61K2039/5154 ,  C12N5/16 ,  C12N13/00 ,  H01L2224/83856 ,  H01L2924/01019

摘要： An object of the invention is to provide a method of treating biological cells prior to subjecting the biological cells to cell fusion pulses which includes the step of treating the biological cells with pre-fusion electric field waveforms which change amplitude in a non-linear way with respect to time, such that the biological cells are first aligned with a relatively low amplitude, long duration pre-fusion electric field waveform and then compressed with a relatively high amplitude, short duration pre-fusion electric field waveform resulting in increased cell membrane contact prior to being subjected to cell fusion. The non-linear pre-fusion electric field waveforms can change in a stepped way, in a continuous way, in a sigmoidal way, with step-wise increasing waveforms in adjacent steps, with step-wise increasing waveforms in non-adjacent steps, and in accordance with non-linear algorithms.

公开(公告)号：EP2411088A1

公开(公告)日：2012-02-01

申请号：EP10756499.9

申请日：2010-03-26

申请人： Cyto Pulse Sciences, Inc.

发明人： WALTERS, Richard, E. ,  WALTERS, Derin, C. ,  KING, Alan, D. ,  MALTAIS, Anna-karin ,  WAHREN, Britta ,  HALLER, Kristian ,  BRAVE, Andreas

IPC分类号： A61N1/30

CPC分类号： A61N1/306 ,  A61K39/00 ,  A61K39/0011 ,  A61K2039/53 ,  A61K2039/54 ,  A61N1/327 ,  C12N2770/24134

摘要： The invention provides a method and apparatus for the delivery of polynucleotide cancer vaccines to increase T cell response and reduce pain due to long electric waveform application and muscle contractions. The method includes the steps of: (a.) administering a polynucleotide cancer vaccine into the skin at an administration site, (b.) applying a needle electrode to the skin in the vicinity of the administration site, and (c.) applying a sequence of at least three electrical waveforms to deliver the polynucleotide cancer vaccine into skin cells by electroporation. The sequence has at least one of the following characteristics {1} at least two of the waveforms differ in amplitude, {2} at least two of the waveforms differ in width, and (3) a first waveform interval for a first set of two of the waveforms is different from a second waveform interval for a second set of two of the waveforms.

公开(公告)号：EP1419244A2

公开(公告)日：2004-05-19

申请号：EP02752177.2

申请日：2002-08-02

申请人： Cyto Pulse Sciences, Inc.

发明人： KING, Alan, D. ,  WALTERS, Richard, E.

IPC分类号： C12N13/00 ,  A61N1/30

CPC分类号： A61N1/325 ,  A61H39/002 ,  A61N1/306

摘要： An object of the invention is to provide a method for delivery of macromolecules into biologicale cel ls in the tissues of a patient and includes the steps of: (a) providing electrodes (16) in an electrode assembly (12), whrein the electrodes have fixed electrode surfaces (42) wich are coated with at least one static layer of electrode releasable molecules (44) to be delivred; (b) providing a waveform generator (15) for generating electric fields; (c) establishing electrically conductive pathways between the electrodes (16) and the waveform generator (15); (d) locating the electrodes (16), such that the biological cells are situated therebetween, and (g) providing electric fields in the form of pulse waveforms from the waveform generator (15) to the electrodes (16), such that molecules in the at least one static layer of the electrode releasable molecules (44) on the electrodes (16) are delivered into the biological cells. The electrode releasable molecules (44) can be either electric field separable molecules and/or solvent separable material. Another object of the invention is to provide an apparatus for carrying out the method of the invention. The static-coated electrode assembly (12) can be provided in a sterile package (24), from wich the electrode assembly (12) is removed prior to use. The statically-coated electrode assembly (12) can be in a form of a disposable assembly (12) wich is removable and replaceable from an electrode assembly holder (13).

公开(公告)号：EP1494752A1

公开(公告)日：2005-01-12

申请号：EP03719469.3

申请日：2003-04-11

申请人： Cyto Pulse Sciences, Inc.

发明人： WALTERS, Richard, E. ,  KING, Alan, D. ,  DEBRUIN, Katherine, A.

IPC分类号： A61N1/04

CPC分类号： A61N1/0412 ,  A61N1/0476 ,  A61N1/306 ,  A61N1/325 ,  A61N1/327

摘要： A method and apparatus are provided for treating biological cellular material with a treating agent using pulsed electrical fields provided by a waveform generator (12). The treatment method includes obtaining an electrode assembly which includes three or more parallel rows of individual electrodes (19). The electrode assembly is applied to a treatment area. Electrically conductive pathways are established between the electrodes (19) and the waveform generator (12) through an array switch (14). Successive electric fields are applied to the treatment area in the form of successive electric field waveforms from the waveform generator (12), through the array switch (14), to adjacent rows of electrodes (19), wherein each successive electric field has the same direction, and wherein polarities of rows of electrodes are reversed successively during the applying of the successive electric fields between adjacent successive rows of electrodes to the treatment area. As a result, the biological cellular material in the treatment area is treated with the treating agent unidirectionally with uniform electric fields with a minimization of the formation of deleterious electrochemistry products at the electrodes.

公开(公告)号：EP1472358A2

公开(公告)日：2004-11-03

申请号：EP02723493.9

申请日：2002-04-05

申请人： Cyto Pulse Sciences, Inc.

发明人： WALTERS, Richard, E. ,  WALTERS, Derin, C. ,  KING, Alan, D.

IPC分类号： C12N15/87

CPC分类号： C12M35/02 ,  A61K2039/5152 ,  A61K2039/5154 ,  C12N5/16 ,  C12N13/00 ,  H01L2224/83856 ,  H01L2924/01019

摘要： An object of the invention is to provide a method of treating biological cells prior to subjecting the biological cells to cell fusion pulses which includes the step of treating the biological cells with pre-fusion electric field waveforms which change amplitude in a non-linear way with respect to time, such that the biological cells are first aligned with a relatively low amplitude, long duration pre-fusion electric field waveform and then compressed with a relatively high amplitude, short duration pre-fusion electric field waveform resulting in increased cell membrane contact prior to being subjected to cell fusion. The non-linear pre-fusion electric field waveforms can change in a stepped way, in a continuous way, in a sigmoidal way, with step-wise increasing waveforms in adjacent steps, with step-wise increasing waveforms in non-adjacent steps, and in accordance with non-linear algorithms.