公开(公告)号：EP1005650A1

公开(公告)日：2000-06-07

申请号：EP99923193.9

申请日：1999-05-19

申请人： DADE BEHRING INC.

发明人： PIRIO, Marcel, Rene ,  DAVALIAN, Dariush ,  ISHKANIAN, Jacqueline, Sadakan ,  ULLMAN, Edwin, F.

IPC分类号： G01N33/532 ,  G01N33/78 ,  G01N33/74

CPC分类号： G01N33/9453 ,  G01N33/532 ,  G01N33/743 ,  G01N33/78 ,  Y10S436/815 ,  Y10S436/817

摘要： The present invention relates to compounds that are bis-biotins. These compounds comprise two biotinyl radicals connected by a chain of atoms, usually at least 16 atoms in length. The bis-biotin is conjugated to a member of a specific binding pair ('sbp member') wherein the chain is not part of the sbp member. Also disclosed are compositions comprising a complex of avidin and a bis-biotin as described above. The compounds and compositions of the invention find use in an assay for an analyte wherein there is employed a reagent system comprising an avidin reagent and a biotin reagent. The improvement of the present invention comprises using as the biotin reagent a bis-biotin as described above. Also disclosed are kits comprising the present bis-biotins and methods of preparing a bis-biotinylated conjugate of a member of a specific binding pair ('sbp member') for use in a specific binding assay.