公开(公告)号：EP1987041A1

公开(公告)日：2008-11-05

申请号：EP07712302.4

申请日：2007-02-23

申请人： DSM IP Assets B.V.

发明人： HANS, Marcus ,  BOVENBERG, Roelof, Ary, Lans ,  VAN DEN BERG, Marco Alexander ,  DRIESSEN, Arnold Jacob Mattieu ,  NIJLAND, Jeroen Gerben

IPC分类号： C07D501/00 ,  C12P35/06 ,  C12N15/81 ,  C12R1/645

CPC分类号： C07K14/37 ,  C12P35/06

摘要： The present invention relates to a cephalosporin-producing microorganism characterized in that it has been transformed with a heterologous CefT gene and whereby the ratio of the cephalosporin production capacity of the microorganism transformed with the heterologous CefT gene compared to the cephalosporin production capacity of the microorganism not being transformed with the heterologous CefT gene is at least 1.2. Furthermore, the present invention provides a method for the production of the cephalosporin-producing microorganism as well as a process for the fermentative production of cephalosporins using the cephalosporin-producing microorganism.

公开(公告)号：EP3004146B1

公开(公告)日：2017-12-20

申请号：EP14728558.9

申请日：2014-06-04

申请人： DSM IP Assets B.V.

发明人： KLAASSEN, Paul ,  DE WAAL, Paulus Petrus ,  DE JONG, René Marcel ,  DRIESSEN, Arnold Jacob Mattieu ,  NIJLAND, Jeroen Gerben ,  SHIN, Hyun Yong

IPC分类号： C07K14/395

CPC分类号： C07K14/395 ,  C12P19/02

公开(公告)号：EP3004146A2

公开(公告)日：2016-04-13

申请号：EP14728558.9

申请日：2014-06-04

申请人： DSM IP Assets B.V.

发明人： KLAASSEN, Paul ,  DE WAAL, Paulus Petrus ,  DE JONG, René Marcel ,  DRIESSEN, Arnold Jacob Mattieu ,  NIJLAND, Jeroen Gerben ,  SHIN, Hyun Yong

IPC分类号： C07K14/395

CPC分类号： C07K14/395 ,  C12P19/02

摘要： The invention relates to a polypeptide having one or more substitution at a position corresponding to position 339 or 376 of SEQ ID NO: 59, wherein the polypeptide is a member of the Major Facilitator Superfamily (MFS). In an embodiment, the substitution is at position corresponding to 376 and wherein the amino acid at that position is replaced by an amino acid that has a van der Waals volume of 80 to 138 A
3 and a side chain hydrophobicity of 10 to 100 Δt
R .

摘要翻译： 本发明涉及在对应于SEQ ID NO：59的位置339或376的位置具有一个或多个取代的多肽，其中所述多肽是主要促进剂超家族（MFS）的成员。 在一个实施方案中，取代位于对应于376的位置，并且其中该位置处的氨基酸被具有范德华体积为80至138 3的氨基酸和10至100重量％的侧链疏水性 。